Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,295,951	T→C	L218L (TTA→TTG)	unknown ←	C4‑dicarboxylate transporter DcuC (TC 2.A.61.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,295,951	0	T	C	100.0%	184.6 / NA	63	L218L (TTA→TTG)	unknown	C4‑dicarboxylate transporter DcuC (TC 2.A.61.1.1)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/31);  total (32/31)

CGACCACCAAGTTATCAAAGGTTTTCTTCGCGCTGAAGTTGCGGGCGAAATCAATCACAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCTAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGC  >  1/1295710‑1296195                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     cGACCACCAAGTTATCAAAGGTTTTCTTCGCGCTGAAGTTGCGGGCGAAATCAATCACAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACg                                                                                                                                                                                                                                              <  1:505637/250‑1 (MQ=255) cGACCACCAAGTTATCAAAGGTTTTCTTCGCGCTGAAGTTGCGGGCGAAATCAATCACAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGt                                                                                                                                                                                                                                             <  1:112746/251‑1 (MQ=255)   accaccAAGTTATCAAAGGTTTTCTTCGCGCTGAAGTTGCGGGCGAAATCAATCACAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTg                                                                                                                                                                                                                                           <  1:746721/251‑1 (MQ=255)             tATCAAAGGTTTTCTTCGCGCTGAAGTTGCGGGCGAAATCAATCACAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGaaaaaa                                                                                                                                                                                                                                 >  1:506028/1‑251 (MQ=255)                               cgcTGAAGCTGCGGGCGAAATCAATCACAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCAGACGCGCCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAAc                                                                                                                                                                                                                                               >  1:441168/1‑219 (MQ=255)                                              cGAAATCAATCACAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAg                                                                                                                                                                                                 <  1:720522/250‑1 (MQ=255)                                                    caatcaCAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAAc                                                                                                                                                                                           >  1:804755/1‑250 (MQ=255)                                                                   tGATAAAACACAGAATAATGATGGTCACGATGTGCGGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTg                                                                                                                                                                              >  1:267986/1‑248 (MQ=255)                                                                   tGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCg                                                                                                                                                                           >  1:230546/1‑251 (MQ=255)                                                                    gATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAAc                                                                                                                                                                                           <  1:36038/234‑1 (MQ=255)                                                                    gATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGa                                                                                                                                                                          >  1:254632/1‑251 (MQ=255)                                                                               gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                               >  1:361850/1‑251 (MQ=255)                                                                               gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                               >  1:44430/1‑251 (MQ=255)                                                                               gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                               >  1:581948/1‑251 (MQ=255)                                                                               gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                               >  1:813586/1‑251 (MQ=255)                                                                               gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                               >  1:197964/1‑251 (MQ=255)                                                                               gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                               >  1:147930/1‑251 (MQ=255)                                                                               gAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCg                                                                                                                                                               >  1:145795/1‑251 (MQ=255)                                                                                        tgGTCACGCTGAGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATACAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATggg                                                                                                                                                      >  1:256070/1‑251 (MQ=255)                                                                                        tgGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTgg                                                                                                                                                                                <  1:693431/225‑1 (MQ=255)                                                                                              cGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCaa                                                                                                                                                                                                            <  1:600702/191‑1 (MQ=255)                                                                                              cGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTgg                                                                                                                                                >  1:884120/1‑251 (MQ=255)                                                                                                aTGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGga                                                                                                                                              >  1:233364/1‑251 (MQ=255)                                                                                                 tgtgCAGTTTCGGTAACCCGAATTTGCCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGgag                                                                                                                                             <  1:356362/251‑1 (MQ=255)                                                                                                 tgtgCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGgag                                                                                                                                             <  1:257710/251‑1 (MQ=255)                                                                                                        tttCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTc                                                                                                                                        <  1:213639/249‑1 (MQ=255)                                                                                                                 cccGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGc                                                                                                                                                                             >  1:381837/1‑203 (MQ=255)                                                                                                                  ccGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAacac                                                                                                                            <  1:748809/251‑1 (MQ=255)                                                                                                                  ccGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAacac                                                                                                                            <  1:855146/251‑1 (MQ=255)                                                                                                                  ccGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAacac                                                                                                                            <  1:842421/251‑1 (MQ=255)                                                                                                                     aaTTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAAcaccac                                                                                                                         <  1:627861/251‑1 (MQ=255)                                                                                                                      aTTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACcaca                                                                                                                        <  1:461802/251‑1 (MQ=255)                                                                                                                          aCCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCAc                                                                                                                    <  1:711255/251‑1 (MQ=255)                                                                                                                             aTCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCgg                                                                                                                 <  1:27155/251‑1 (MQ=255)                                                                                                                                 aaaaGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCAc                                                                                                                    <  1:399006/244‑1 (MQ=255)                                                                                                                                   aaGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCg                                                                                                           >  1:216900/1‑251 (MQ=255)                                                                                                                                             ccGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGc                                                                                                                                                                             >  1:281134/1‑175 (MQ=255)                                                                                                                                                     gggTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGGAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGaaa                                                                                         >  1:453919/1‑251 (MQ=255)                                                                                                                                                     gggTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGaaa                                                                                         >  1:611969/1‑251 (MQ=255)                                                                                                                                                       gTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTCCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAAt                                                                                        <  1:425538/250‑1 (MQ=255)                                                                                                                                                              cGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGCGACAAAATAATGGAAATCGGTGAgg                                                                                >  1:730412/1‑251 (MQ=255)                                                                                                                                                                    aaTCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACaaa                                                                          <  1:211627/251‑1 (MQ=255)                                                                                                                                                                          gTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATc                                                                                                                      >  1:554234/1‑201 (MQ=255)                                                                                                                                                                          gTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCgtaaaacgaatgtccgtg                                                                     >  1:882511/1‑233 (MQ=255)                                                                                                                                                                                   cGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCAc                                                           <  1:36539/251‑1 (MQ=255)                                                                                                                                                                                   cGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCAc                                                           <  1:31129/251‑1 (MQ=255)                                                                                                                                                                                           tGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCgagag                                                   <  1:703168/251‑1 (MQ=255)                                                                                                                                                                                           tGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCgagag                                                   <  1:779065/251‑1 (MQ=255)                                                                                                                                                                                             gTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTggg                                                                                                                                               >  1:780799/1‑157 (MQ=255)                                                                                                                                                                                                          aCGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGAcc                                    <  1:449245/251‑1 (MQ=255)                                                                                                                                                                                                             cgTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATgg                                                                                            >  1:205344/1‑192 (MQ=255)                                                                                                                                                                                                             cgTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGAcc                                    <  1:579996/248‑1 (MQ=255)                                                                                                                                                                                                               tCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGAc                               <  1:403890/251‑1 (MQ=255)                                                                                                                                                                                                                      gTTCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGAt                                                                                                                                                    >  1:852131/1‑127 (MQ=255)                                                                                                                                                                                                                       ttCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAAc                        <  1:630617/250‑1 (MQ=255)                                                                                                                                                                                                                       ttCGATTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAAc                        <  1:825627/250‑1 (MQ=255)                                                                                                                                                                                                                           aTTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGAt                                                                                                                                                    >  1:779963/1‑122 (MQ=255)                                                                                                                                                                                                                           aTTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGt                                                                                                             >  1:810585/1‑161 (MQ=255)                                                                                                                                                                                                                           aTTTTAATGCCTTCTTTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGt                   >  1:283661/1‑251 (MQ=255)                                                                                                                                                                                                                                        cttTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCGCCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAAt      <  1:474270/251‑1 (MQ=255)                                                                                                                                                                                                                                        cttTGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAAt      >  1:437084/1‑251 (MQ=255)                                                                                                                                                                                                                                          ttGCGATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATAc    <  1:204977/251‑1 (MQ=255)                                                                                                                                                                                                                                              gATCCAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGc  >  1:656779/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CGACCACCAAGTTATCAAAGGTTTTCTTCGCGCTGAAGTTGCGGGCGAAATCAATCACAGCGGTAATGATAAAACACAGAATAATGATGGTCACGATGTGCAGTTTCGGTAACCCGAATTTACCATCGAAAAGTAACACGCCGATGATGGGTAAAAGCGGTAGAATCGCGTAGAAATTCGGCGCGATGGTTTTGATTTCGGACGCGTCAACCCGTTCGATTTTAATGCCTTCTTTGCGATCTAAATAACGTTGCCAGAAAAAATGGGCGACGGCGATGCCGCAAATGGCGAAAAGGGAAACCGGCAGGCTGGTGCCGAAAGCAAATTCGCTTAATGGGATTTGGGAGATTTCGGCGGATAACACCACATCACCGGAGGTCGGTGACAAAATAATGGAAATCGGTGAGGCACAAATGGCTGCCGCCGCACCTCGAGAGATGCCGACATTGACCATGACGGGGAACAGGGTCGCCATTAACAATACGC  >  1/1295710‑1296195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑