Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,295,512	T→C	S365G (AGC→GGC)	unknown ←	C4‑dicarboxylate transporter DcuC (TC 2.A.61.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,295,512	0	T	C	100.0%	224.8 / NA	77	S365G (AGC→GGC)	unknown	C4‑dicarboxylate transporter DcuC (TC 2.A.61.1.1)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/37);  total (40/37)

GGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCTGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCCGCGATAGGCGACCACCAAGTTATCAAAGGTTTTCTTCGCG  >  1/1295276‑1295741                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      ggCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCa                                                                                                                                                                                                                         >  1:726062/1‑251 (MQ=255)      tAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCAtggtg                                                                                                                                                                                                                    >  1:294311/1‑251 (MQ=255)              caAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAAt                                                                                                                                                                                                            <  1:338386/251‑1 (MQ=255)              caAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAAt                                                                                                                                                                                                            <  1:293253/251‑1 (MQ=255)                aaTGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATgg                                                                                                                                                                                                          >  1:181052/1‑251 (MQ=255)                aaTGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATgg                                                                                                                                                                                                          >  1:288144/1‑251 (MQ=255)                   gAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGtga                                                                                                                                                                                                       >  1:35120/1‑251 (MQ=255)                                    gCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTaa                                                                                                                                                                                       >  1:594003/1‑250 (MQ=255)                                     cACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAAca                                                                                                                                                                                     <  1:813776/251‑1 (MQ=255)                                     cACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAAc                                                                                                                                                                                      <  1:486192/250‑1 (MQ=255)                                     cACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAAc                                                                                                                                                                                      <  1:355181/250‑1 (MQ=255)                                      aCGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAAcat                                                                                                                                                                                    <  1:718860/251‑1 (MQ=255)                                            aTTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGt                                                                                                                                                                              <  1:703427/251‑1 (MQ=255)                                              tCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTa                                                                                                                                                                            >  1:845094/1‑251 (MQ=255)                                                        aTTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCtgctgc                                                                                                                                                                   >  1:651123/1‑250 (MQ=255)                                                                  gagagaTTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGt                                                                                                                                                                              >  1:746919/1‑229 (MQ=255)                                                                      gaTTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAAcatcat                                                                                                                                                                                 <  1:725526/222‑1 (MQ=255)                                                                                aTGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAAca                                                                                                                                                                                     >  1:92518/1‑208 (MQ=255)                                                                                aTGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGAt                                                                                                                                                        <  1:897730/237‑1 (MQ=255)                                                                                aTGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAAt                                                                                                                                          >  1:124289/1‑251 (MQ=255)                                                                                aTGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAAt                                                                                                                                          >  1:500700/1‑251 (MQ=255)                                                                                 tGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATg                                                                                                                                         <  1:176301/251‑1 (MQ=255)                                                                                 tGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATg                                                                                                                                         <  1:891213/251‑1 (MQ=255)                                                                                  gCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAAca                                                                                                                                                                                     <  1:597389/206‑1 (MQ=255)                                                                                  gCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCg                                                                                                                                                  <  1:57000/241‑1 (MQ=255)                                                                                      gACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGAt                                                                                                                                     <  1:249977/250‑1 (MQ=255)                                                                                         acCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGt                                                                                                                                 >  1:677450/1‑251 (MQ=255)                                                                                                ccACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGAt                                                                                                                              <  1:106074/247‑1 (MQ=255)                                                                                                     aCGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAAt                                                                                                                     >  1:811978/1‑251 (MQ=255)                                                                                                      cGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATg                                                                                                                                         >  1:241200/1‑230 (MQ=255)                                                                                                        ccAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAg                                                                                                                   <  1:407590/250‑1 (MQ=255)                                                                                                          aGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTg                                                                                                                 >  1:532218/1‑250 (MQ=255)                                                                                                               ccGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCAtgg                                                                                                                                                                                                                      <  1:236036/144‑1 (MQ=255)                                                                                                                cGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAAc                                                                                                          <  1:380749/251‑1 (MQ=255)                                                                                                                 ggTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAg                                                                                                                   >  1:532392/1‑241 (MQ=255)                                                                                                                  gTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGtgatg                                                                                                                                                                                                     <  1:339436/158‑1 (MQ=255)                                                                                                                  gTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTa                                                                                                                                                                            <  1:200862/183‑1 (MQ=255)                                                                                                                  gTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAAc                                                                                                          <  1:544134/249‑1 (MQ=255)                                                                                                                  gTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACtt                                                                                                        >  1:299175/1‑251 (MQ=255)                                                                                                                            gcgACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATa                                                                                              >  1:876263/1‑251 (MQ=255)                                                                                                                              gACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGGTTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACg                                                                                            >  1:848137/1‑251 (MQ=255)                                                                                                                               aCCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAg                                                                                                                                                                               >  1:516280/1‑167 (MQ=255)                                                                                                                               aCCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAg                                                                                                                   >  1:310890/1‑227 (MQ=255)                                                                                                                                ccTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGcc                                                                                          <  1:261566/251‑1 (MQ=255)                                                                                                                                   aaGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTg                                                                                        >  1:713520/1‑250 (MQ=255)                                                                                                                                    aGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTgcg                                                                                      >  1:343547/1‑251 (MQ=255)                                                                                                                                      ttGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTgcgg                                                                                     <  1:36323/250‑1 (MQ=255)                                                                                                                                           ggCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAg                                                                                                                   <  1:655190/215‑1 (MQ=255)                                                                                                                                           ggCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGGGCTTAAAc                                                                                                          >  1:332443/1‑224 (MQ=255)                                                                                                                                           ggCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAG‑TAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCaa                                                                              >  1:468499/1‑251 (MQ=255)                                                                                                                                             cTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGt                                                                                                                                                                              >  1:562526/1‑154 (MQ=255)                                                                                                                                              ttGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGAc                                                                                                                                                                                                   >  1:372732/1‑132 (MQ=255)                                                                                                                                               tGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAg                                                                           <  1:607585/251‑1 (MQ=255)                                                                                                                                                gTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAg                                                                           >  1:626167/1‑250 (MQ=255)                                                                                                                                                    cATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTg                                                                                                                 <  1:306390/208‑1 (MQ=255)                                                                                                                                                    cATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTgcgg                                                                                     >  1:570098/1‑236 (MQ=255)                                                                                                                                                      tCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGAGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATcac                                                                     >  1:434672/1‑250 (MQ=255)                                                                                                                                                       cGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATcacca                                                                   <  1:71649/251‑1 (MQ=255)                                                                                                                                                            tAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATg                                                                                                                                         >  1:599088/1‑176 (MQ=255)                                                                                                                                                              gTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGc                                                                                                                                                   <  1:518048/164‑1 (MQ=255)                                                                                                                                                              gTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCgg                                                             >  1:775544/1‑250 (MQ=255)                                                                                                                                                                     aaGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCg                                                     <  1:576031/251‑1 (MQ=255)                                                                                                                                                                      aGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACtt                                                                                                        >  1:34477/1‑199 (MQ=255)                                                                                                                                                                           gATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGcc                                                                                                                                                                  <  1:827734/136‑1 (MQ=255)                                                                                                                                                                               ggCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTg                                                                                                                                                      <  1:399872/144‑1 (MQ=255)                                                                                                                                                                                gcgcCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAgctg                                                                                                                                                                       >  1:605696/1‑126 (MQ=255)                                                                                                                                                                                 cgcCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCcg                                         <  1:876331/251‑1 (MQ=255)                                                                                                                                                                                 cgcCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCcg                                         <  1:863678/251‑1 (MQ=255)                                                                                                                                                                                     cATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGAc                                                                                                                                                                                                   >  1:483260/1‑93 (MQ=255)                                                                                                                                                                                     cATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAg                                                                                                                   <  1:680983/173‑1 (MQ=255)                                                                                                                                                                                                ccAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCCGCGATAGGCGaccac                           <  1:109161/250‑1 (MQ=255)                                                                                                                                                                                                  aGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCCGCGATa                                    >  1:470023/1‑239 (MQ=255)                                                                                                                                                                                                    tttCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCCGCGATAGGCGACCACCAAg                       <  1:407321/250‑1 (MQ=255)                                                                                                                                                                                                    tttCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCCGCGATAGGCGACCACCAAg                       <  1:507090/250‑1 (MQ=255)                                                                                                                                                                                                     ttCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAgc                                                                                                                                                                         >  1:521523/1‑103 (MQ=255)                                                                                                                                                                                                                         aGCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCCGCGATAGGCGACCACCAAGTTATCAAAGGTTTTCTTcgcg  >  1:671024/1‑250 (MQ=255)                                                                                                                                                                                                                          gCGTAGAATGCCGCGTTGCCGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAg                                                                           >  1:4716/1‑176 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      GGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATCAGTTCGGCGAAAGCGTAGAATGCCGCGTTGCTGGAGCCGGTCGCCATGGTGGCTAAAATGGTGATGACGGCGAGTACTAACATCATAGTTAAGCTGCTGCCACCGAAAGATTGGGCGGAATCAATGAGATTGGTGATAAAACCAATAGTGCTTAAACTTTGTGCGAATACGCCTGCGGCGACCAATAGCATCACCACGCCGGCAAAGGCGTCCGCCATGCCGCGATAGGCGACCACCAAGTTATCAAAGGTTTTCTTCGCG  >  1/1295276‑1295741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑