Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,295,237	A→G	intergenic (‑15/+15)	unknown ← / ← unknown	hypothetical protein/C4‑dicarboxylate transporter DcuC (TC 2.A.61.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,295,237	0	A	G	100.0%	195.6 / NA	68	intergenic (‑15/+15)	unknown/unknown	hypothetical protein/C4‑dicarboxylate transporter DcuC (TC 2.A.61.1.1)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/34);  total (34/34)

CTACATGGTGAAATACGAGGAAATTATACGTCAATCGTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCACACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATC  >  1/1295007‑1295480                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cTACATGGTGAAATACGAGGAAATTATACGTCAATCGTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTTTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGc                                                                                                                                                                                                                                 <  1:780811/251‑1 (MQ=255)     aTGGTGAAATACGAGGAAATTATACGTCAATCGTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGga                                                                                                                                                                                                                             >  1:608384/1‑251 (MQ=255)     aTGGTGAAATACGAGGAAATTATACGTCAATCGTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGga                                                                                                                                                                                                                             >  1:873175/1‑251 (MQ=255)                    gAAATTATACGTCAATCGTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCtgtg                                                                                                                                                                                                              >  1:456681/1‑251 (MQ=255)                             cGTCAATCGTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAAcc                                                                                                                                                                                                     <  1:579318/251‑1 (MQ=255)                                  aTCGTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACcacaca                                                                                                                                                                                                >  1:535048/1‑251 (MQ=255)                                     gTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCgcac                                                                                                                                                                                                                                                  >  1:513407/1‑198 (MQ=255)                                     gTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAgg                                                                                                                                                                                                                                  <  1:369688/214‑1 (MQ=255)                                      tATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATg                                                                                                                                                                                            >  1:880642/1‑251 (MQ=255)                                        ttGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAActc                                                                                                                                                                                                                  <  1:753944/227‑1 (MQ=255)                                        ttGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGaa                                                                                                                                                                                          >  1:363806/1‑251 (MQ=255)                                            aatgcaatACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACTACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAAccc                                                                                                                                                                                      <  1:614349/251‑1 (MQ=255)                                            aatgcaatACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAGccc                                                                                                                                                                                      <  1:199018/251‑1 (MQ=255)                                            aatgcaatACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAAccc                                                                                                                                                                                      <  1:395303/251‑1 (MQ=255)                                                  atACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACgg                                                                                                                                                                                                                                           <  1:867780/192‑1 (MQ=255)                                                  atACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGc                                                                                                                                                                                                                                 >  1:702525/1‑202 (MQ=255)                                                    aCAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAAcc                                                                                                                                                                                       >  1:580186/1‑242 (MQ=255)                                                    aCAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCAt                                                                                                                                                                              >  1:276629/1‑251 (MQ=255)                                                               cgAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGAc                                                                                                                                                                    <  1:584949/250‑1 (MQ=255)                                                                            cACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTgg                                                                                                                                                                                                             <  1:559164/196‑1 (MQ=255)                                                                            cACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGAt                                                                                                                                                      >  1:187971/1‑251 (MQ=255)                                                                                   ttATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAAc                                                                                                                                               <  1:789030/251‑1 (MQ=255)                                                                                                 ctctctTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGc                                                                                                                                 <  1:42193/251‑1 (MQ=255)                                                                                                  tctctTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGcc                                                                                                                                <  1:129490/251‑1 (MQ=255)                                                                                                   ctctTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAGCGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCTCCGCACTTTACGGTGAATTAGGCGGGAATGAATAActc                                                                                                                                                                                                                  <  1:287685/168‑1 (MQ=255)                                                                                                     ctTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGc                                                                                                                                 >  1:466770/1‑247 (MQ=255)                                                                                                     ctTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATg                                                                                                                             >  1:867402/1‑251 (MQ=255)                                                                                                        gTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCg                                                                                                                          >  1:506526/1‑251 (MQ=255)                                                                                                           aaaCGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGac                                                                                                                       <  1:582955/251‑1 (MQ=255)                                                                                                           aaaCGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGac                                                                                                                       <  1:25695/251‑1 (MQ=255)                                                                                                           aaaCGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGac                                                                                                                       <  1:694901/251‑1 (MQ=255)                                                                                                             aCGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGaca                                                                                                                      >  1:745687/1‑250 (MQ=255)                                                                                                                        ttCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGgaa                                                                                                                                                                                                                            <  1:391705/137‑1 (MQ=255)                                                                                                                        ttCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTa                                                                                                          >  1:619805/1‑251 (MQ=255)                                                                                                                        ttCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTa                                                                                                          >  1:373267/1‑251 (MQ=255)                                                                                                                        ttCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTa                                                                                                          >  1:107168/1‑251 (MQ=255)                                                                                                                            aCCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGcc                                                                                                      <  1:348371/251‑1 (MQ=255)                                                                                                                                        gAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGa                                                                                          >  1:123529/1‑251 (MQ=255)                                                                                                                                             aCGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAAccc                                                                                                                                                                                      >  1:879985/1‑154 (MQ=255)                                                                                                                                             aCGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAg                                                                                      >  1:37688/1‑250 (MQ=255)                                                                                                                                             aCGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAg                                                                                      >  1:899638/1‑250 (MQ=255)                                                                                                                                             aCGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTCTTTAGTGCAGAAAAACATCGGGAAAAACCACCGCACTTTACGGCGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATCCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAg                                                                                      >  1:416602/1‑250 (MQ=255)                                                                                                                                                      aTTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGc                                                                                     <  1:82533/242‑1 (MQ=255)                                                                                                                                                         gCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCgcac                                                                                                                                                                                                                                                  >  1:589354/1‑82 (MQ=255)                                                                                                                                                          cGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGt                                                                         <  1:285523/250‑1 (MQ=255)                                                                                                                                                            gtgtTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTgg                                                                                                                                                                                                             <  1:817073/116‑1 (MQ=255)                                                                                                                                                              gtTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGacac                                                                                                                     >  1:590988/1‑202 (MQ=255)                                                                                                                                                              gtTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGAc                                                                                                               <  1:767242/208‑1 (MQ=255)                                                                                                                                                              gtTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAg                                                                    >  1:374219/1‑251 (MQ=255)                                                                                                                                                              gtTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAg                                                                    >  1:522890/1‑251 (MQ=255)                                                                                                                                                                       ccgtaaaGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTaa                                                            >  1:825712/5‑250 (MQ=255)                                                                                                                                                                       aacaaaAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAAc                                                           <  1:465106/251‑1 (MQ=255)                                                                                                                                                                       aacaaaAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAAc                                                           <  1:643021/251‑1 (MQ=255)                                                                                                                                                                         caaaAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTgg                                                                      >  1:622571/1‑238 (MQ=255)                                                                                                                                                                         caaaAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACAt                                                         >  1:813818/1‑251 (MQ=255)                                                                                                                                                                               tGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGaa                                                    <  1:609922/250‑1 (MQ=255)                                                                                                                                                                                gCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAAc                                                           <  1:276069/242‑1 (MQ=255)                                                                                                                                                                                     ggATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGac                                                                                                  >  1:637628/1‑198 (MQ=255)                                                                                                                                                                                     ggATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTaa                                             >  1:876766/1‑251 (MQ=255)                                                                                                                                                                                       aTTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAAt                                           <  1:611669/251‑1 (MQ=255)                                                                                                                                                                                        tttCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGacac                                                                                                >  1:477756/1‑197 (MQ=255)                                                                                                                                                                                            aCGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGcc                                      <  1:524667/251‑1 (MQ=255)                                                                                                                                                                                                             gtAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATa                                                                                        >  1:341445/1‑184 (MQ=255)                                                                                                                                                                                                             gtAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGtt                                                                        <  1:355471/200‑1 (MQ=255)                                                                                                                                                                                                              tAGAAAAACATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAGGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTg                     >  1:215926/1‑250 (MQ=255)                                                                                                                                                                                                                 aaaaaCATCGGGAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACGCCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTgcgc                  <  1:611324/250‑1 (MQ=255)                                                                                                                                                                                                                          gggAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATa                                                                                        <  1:652703/171‑1 (MQ=255)                                                                                                                                                                                                                            gAAAAACCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGc                                                                  >  1:494684/1‑191 (MQ=255)                                                                                                                                                                                                                              aaaaCCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGa    <  1:321804/251‑1 (MQ=255)                                                                                                                                                                                                                                aaCCACCGCACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATc  <  1:743429/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CTACATGGTGAAATACGAGGAAATTATACGTCAATCGTATTGCAATGCAATACAAAATAGCGCGAATTTTAACCGCACTTGCTTATGATTTTTTGTCTCTCTTGTAAAACGGTGGCGCATTCTACCACTTCCCTTGAATAACGTAAACGATTGCGGTGTTTTGGCAAACAAAAGTGCGGTGGATTTCACGGGTGTTTTTTTAGTGTAGAAAAACATCGGGAAAAACCACCACACTTTACGGTGAATTAGGCGGGAATGAATAACTCTGTGGCGATAACCACACAAATGAAACCCACCGCCATCGGCACGGACATTCGTTTTACGATTTCAAACGGAGAGATTTTCGCCATGCCGGACACCGCGACCACTACGCCAGACACCGGTGATAAGCCGCGACCTAAGTTGGAGGCTTGTAACATCGGAATAGTTAAATAAGCCGGATTGGCGCCCATTTGCGCCGCCAGTTTCGGGATC  >  1/1295007‑1295480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑