Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,292,594 T→G intergenic (+63/+262) unknown → / ← unknown hypothetical protein/GMP synthase [glutamine‑hydrolyzing], amidotransferase subunit (EC 6.3.5.2) / GMP synthase [glutamine‑hydrolyzing], ATP pyrophosphatase subunit (EC 6.3.5.2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,292,5940TG100.0% 83.2 / NA 30intergenic (+63/+262)unknown/unknownhypothetical protein/GMP synthase [glutamine‑hydrolyzing], amidotransferase subunit (EC 6.3.5.2) / GMP synthase [glutamine‑hydrolyzing], ATP pyrophosphatase subunit (EC 6.3.5.2)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/17);  total (13/17)

GAATGGTTAGTCGTAGCGGAATGTTATTGTTCCCGAGGGTGTTACGGATACATAAAGCCCATCACGATCGGCTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAAT  >  1/1292522‑1292826
                                                                        |                                                                                                                                                                                                                                        
gattctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTttatta                                                                                                                                                                          >  1:530028/8‑137 (MQ=255)
 attctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaac                                                       <  1:866294/245‑10 (MQ=255)
 attctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaa                                                        >  1:348179/7‑242 (MQ=255)
    ctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATAc                                                                                                                                                              <  1:255045/142‑1 (MQ=255)
         gTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAg                                                                                                                                                   <  1:152156/151‑1 (MQ=255)
         gTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCaa                                                                                                               >  1:32497/1‑187 (MQ=255)
                      gtgatagtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAAccc                                   >  1:848490/25‑250 (MQ=255)
                            gtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGAc                               >  1:312119/19‑248 (MQ=255)
                            gtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATa                            >  1:882325/19‑251 (MQ=255)
                             tccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACACCGACAt                             <  1:301516/232‑1 (MQ=255)
                               cctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgag                         <  1:86361/236‑3 (MQ=255)
                                ctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                        <  1:521015/237‑4 (MQ=255)
                                ctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                        <  1:567246/237‑4 (MQ=255)
                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTa                                                                                                                                           >  1:141019/5‑126 (MQ=255)
                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATAc                                                                         <  1:468338/188‑1 (MQ=255)
                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaac                                                       <  1:455610/206‑10 (MQ=255)
                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAAcccc                                  <  1:729126/227‑1 (MQ=255)
                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaa              >  1:632161/5‑249 (MQ=255)
                                           actgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac             <  1:485276/248‑4 (MQ=255)
                                           actgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac             <  1:700471/248‑4 (MQ=255)
                                            ctgattcATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                        <  1:713484/233‑4 (MQ=255)
                                              gATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATAc                                                                         >  1:165059/1‑188 (MQ=255)
                                               aTACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaactatt         >  1:775076/1‑244 (MQ=255)
                                                tACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGtg                                                                                                                                                                                                           <  1:318317/56‑1 (MQ=255)
                                                 aCATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatga                                                                    <  1:421774/190‑1 (MQ=255)
                                                  cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATAc                                                                                                                                                              <  1:539925/99‑1 (MQ=255)
                                                  cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAGTAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTg      >  1:447236/1‑251 (MQ=255)
                                                  cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTg      >  1:876352/1‑251 (MQ=255)
                                                  cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGAATTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaat                                                                 >  1:93961/1‑192 (MQ=255)
                                                            gtCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGaaac  <  1:576753/244‑2 (MQ=255)
                                                                        |                                                                                                                                                                                                                                        
GAATGGTTAGTCGTAGCGGAATGTTATTGTTCCCGAGGGTGTTACGGATACATAAAGCCCATCACGATCGGCTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAAT  >  1/1292522‑1292826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑