Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,289,636	C→T	V300V (GTG→GTA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,289,636	0	C	T	100.0%	66.8 / NA	25	V300V (GTG→GTA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/16);  total (9/16)

AATGTACTAAATGTTCATATTTAATTGATTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGCACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGATAAAAATGTGAACATTT  >  1/1289405‑1289839                                                                                                                                                                                                                                        |                                                                                                                                                                                                            aaTGTATTAAATGTTCATATTTAATTGATTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTgg                                                                                                                                                                                          <  1:350309/251‑1 (MQ=255)    gtattAAATGTTCATATTTAATTGATTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAg                                                                                                                                                                                       >  1:291983/5‑251 (MQ=255)                            aTTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACa                                                                                                                                                               <  1:145024/251‑1 (MQ=255)                                                 cAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAAc                                                                                                                                          <  1:399670/251‑1 (MQ=255)                                                                  ttGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACAt                                                                                                                                                              <  1:538589/214‑1 (MQ=255)                                                                  ttGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAat                                                                                                                                               >  1:265624/1‑229 (MQ=255)                                                                         aTTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTCTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTa                                                                                                                                        <  1:286851/229‑1 (MQ=255)                                                                                  cTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAAttagtt                                                                                                                                                                                                >  1:86749/1‑164 (MQ=255)                                                                                    aGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAAc                                                                                                                                          <  1:140500/216‑1 (MQ=255)                                                                                              aaaaaGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAAc                                                                                                       <  1:882210/241‑1 (MQ=255)                                                                                                aaaGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAAt                                                                                           <  1:873516/251‑1 (MQ=255)                                                                                                                                                             gCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCaaa                               <  1:135644/250‑1 (MQ=255)                                                                                                                                                                        gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAAc                                                                                                                                                                <  1:129688/110‑1 (MQ=255)                                                                                                                                                                        gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACAt                                                                                                                                                              >  1:326978/1‑112 (MQ=255)                                                                                                                                                                        gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAgg                                                                                                                                      <  1:117301/136‑1 (MQ=255)                                                                                                                                                                        gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGc                                                                                 <  1:827236/189‑1 (MQ=255)                                                                                                                                                                        gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGa                   >  1:186542/1‑251 (MQ=255)                                                                                                                                                                                   gATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACa                                                                                                                                                               >  1:308016/1‑100 (MQ=255)                                                                                                                                                                                   gATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGATAAAAATGTGa        >  1:295589/1‑251 (MQ=255)                                                                                                                                                                                        aGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGt                                                                                                                     <  1:11667/137‑1 (MQ=255)                                                                                                                                                                                         gATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGATAAAAATGTGAACAttt  >  1:19038/1‑251 (MQ=255)                                                                                                                                                                                          aTCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAat                                                                                                                                               <  1:786794/109‑1 (MQ=255)                                                                                                                                                                                                                   aGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAAcc                                         <  1:557894/186‑1 (MQ=255)                                                                                                                                                                                                                               ttattGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGt                                                                                                                     >  1:832624/1‑98 (MQ=255)                                                                                                                                                                                                                               ttattGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAAcc                                                                                                      <  1:68850/113‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                            AATGTACTAAATGTTCATATTTAATTGATTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGCACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGATAAAAATGTGAACATTT  >  1/1289405‑1289839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑