Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,285,445	A→C	I28M (ATT→ATG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,285,445	0	A	C	100.0%	157.7 / NA	53	I28M (ATT→ATG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/25);  total (28/25)

AATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGATATAATGCAATGATTTATAAATGAATAAAAATGATATTGTTTATTCTCTACCGTCATTTTTACCGTCACCAGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGGCCCATAGACGCTTTAACACTTTTGGGGAATTTACCTTGTTTAATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAAGAGGTATTTTTGTG  >  1/1285219‑1285685                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 aaTTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCg                                                                                                                                                                                                                          >  1:353600/1‑251 (MQ=255)                 gAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACtt                                                                                                                                                                                                          >  1:534059/1‑251 (MQ=255)                  aaGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTcatcat                                                                                                                                                                                                                                             >  1:573166/1‑215 (MQ=255)                              cGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTg                                                                                                                                                                                              >  1:811968/1‑250 (MQ=255)                                   atttatCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAg                                                                                                                                                                                                                    <  1:186895/223‑1 (MQ=255)                                   atttatCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTa                                                                                                                                                                                         >  1:113098/1‑250 (MQ=255)                                         cGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTtagta                                                                                                                                                                                  >  1:787709/1‑251 (MQ=255)                                                                      aaTAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTtctc                                                                                                                                                     <  1:791741/251‑1 (MQ=255)                                                                                    tGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACGCGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAAtt                                                                                                                                                                                                                                   <  1:844659/159‑1 (MQ=255)                                                                                    tGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCtt                                                                                                                                                                                           <  1:78960/199‑1 (MQ=255)                                                                                     gTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTtagtag                                                                                                                                                                                 <  1:208337/208‑1 (MQ=255)                                                                                     gTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAg                                                                                                                                      >  1:825922/1‑251 (MQ=255)                                                                                         aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGTTTTTATCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCa                                                                                                                                  >  1:412077/1‑251 (MQ=255)                                                                                         aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCtt                                                                                                                                                                                           <  1:730566/194‑1 (MQ=255)                                                                                         aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCa                                                                                                                                  >  1:340052/1‑251 (MQ=255)                                                                                         aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCAATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCt                                                                                                                                                                                                                  >  1:361858/1‑171 (MQ=255)                                                                                          ttCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCAt                                                                                                                                 >  1:488936/1‑251 (MQ=255)                                                                                                  cGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGc                                                                                                                         <  1:412717/251‑1 (MQ=255)                                                                                                       tttttaccgtctttggaagaaAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGtt                                                                                                                    >  1:142387/16‑251 (MQ=255)                                                                                                       tttttaccgtctttggaagaaAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGtt                                                                                                                    >  1:640421/16‑251 (MQ=255)                                                                                                                tctttggaagaaAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTGCCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTGCTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCa                                                                                                           <  1:249440/245‑1 (MQ=255)                                                                                                                                 cATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAatat                                                                                          >  1:336520/1‑251 (MQ=255)                                                                                                                                  aTCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACtt                                                                                                                                                                                      <  1:884412/158‑1 (MQ=255)                                                                                                                                  aTCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATAtt                                                                                         >  1:287794/1‑251 (MQ=255)                                                                                                                                    cAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAAttgttg                                                                                                                                                                                                                               >  1:683844/1‑115 (MQ=255)                                                                                                                                       gAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATAttt                                                                                        <  1:15111/247‑1 (MQ=255)                                                                                                                                                  aaGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGc                                                                         <  1:417985/251‑1 (MQ=255)                                                                                                                                                    gCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAAt                                                                                                                                                                                                                                    <  1:846544/94‑1 (MQ=255)                                                                                                                                                         gTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTc                                                                                                                              <  1:397612/191‑1 (MQ=255)                                                                                                                                                               atatCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCACGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCt                                                             >  1:246589/1‑250 (MQ=255)                                                                                                                                                                                  cTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTc                                                            <  1:671454/232‑1 (MQ=255)                                                                                                                                                                                  cTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTG‑‑‑‑‑CTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTtata                                    >  1:68068/1‑251 (MQ=37)                                                                                                                                                                                       cgccccATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTtata                                    >  1:387462/4‑251 (MQ=255)                                                                                                                                                                                         ccccATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGcc                                                                                                                                    <  1:832729/152‑1 (MQ=255)                                                                                                                                                                                         ccccATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATAc                                   >  1:82326/2‑250 (MQ=255)                                                                                                                                                                                         ccccATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATAc                                   <  1:67171/249‑1 (MQ=255)                                                                                                                                                                                         ccccATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACa                                  >  1:593200/2‑251 (MQ=255)                                                                                                                                                                                         ccccATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACa                                  >  1:525486/2‑251 (MQ=255)                                                                                                                                                                                           ccATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATAc                                   >  1:661920/1‑248 (MQ=255)                                                                                                                                                                                                    cTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGc                                                                                                                         >  1:58415/1‑153 (MQ=255)                                                                                                                                                                                                    cTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTg                       >  1:700127/1‑251 (MQ=255)                                                                                                                                                                                                    cTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTg                       <  1:896429/251‑1 (MQ=255)                                                                                                                                                                                                    cTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTg                       >  1:77298/1‑251 (MQ=255)                                                                                                                                                                                                    cTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTg                       <  1:669425/251‑1 (MQ=255)                                                                                                                                                                                                            cTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTATTTAGTAGCGCTGGTTGTTGTTCCGTTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAag               <  1:455956/251‑1 (MQ=255)                                                                                                                                                                                                            cTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAag               <  1:107498/251‑1 (MQ=255)                                                                                                                                                                                                            cTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAag               <  1:307180/251‑1 (MQ=255)                                                                                                                                                                                                                   ggAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATAc                                   <  1:207561/224‑1 (MQ=255)                                                                                                                                                                                                                    gAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATAc                                   <  1:407199/223‑1 (MQ=255)                                                                                                                                                                                                                     aaTTTTCCTTGTCTCATCATACGTCTAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAATCCCATTTCTGGGCTTGTTATCTAACCAATAGCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAAGAGGTAttt       <  1:870811/250‑1 (MQ=25)                                                                                                                                                                                                                       tttACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTAc                                                                                                                                                                                        >  1:20552/1‑71 (MQ=255)                                                                                                                                                                                                                       tttACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAAGAGGTATTTTtg    <  1:410851/251‑1 (MQ=255)                                                                                                                                                                                                                          aCCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAAGAGGTATTTTtgtg  >  1:711774/1‑250 (MQ=255)                                                                                                                                                                                                                          aCCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAAGAGGTATTTTtgtg  >  1:509248/1‑250 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 AATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGATATAATGCAATGATTTATAAATGAATAAAAATGATATTGTTTATTCTCTACCGTCATTTTTACCGTCACCAGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGGCCCATAGACGCTTTAACACTTTTGGGGAATTTACCTTGTTTAATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCATTTCTGGGCTTGTTATCTAACCAATATCGCGATTTAATATTTCGCGGATCTTGTCGCCTGATTTCTGCTCAATGAAATCATTTATCACTTTATACAATCGCCATTTGTATTTAAGAGGTATTTTTGTG  >  1/1285219‑1285685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑