Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,280,297	G→T	A268A (GCC→GCA)	unknown ←	Pyridoxal kinase (EC 2.7.1.35)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,280,297	0	G	T	100.0%	225.3 / NA	75	A268A (GCC→GCA)	unknown	Pyridoxal kinase (EC 2.7.1.35)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (37/38);  total (37/38)

CAACTGAAAGTGCGGTCGATTTTCCCGGTGTTTGGCAAATAAGGTGAGAAGGGCGGTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGGGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCAGTAGCAAGCAACATTTCAAATTGATTG  >  1/1280053‑1280534                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              cAACTGAAAGTGCGGTCGATTTTCCCGGTGTTTGGCAAATAAGGTGAGAAGGGCGGTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTgcggcg                                                                                                                                                                                                                                         >  1:686151/1‑251 (MQ=255) cAACTGAAAGTGCGGTCGATTTTCCCGGTGTTTGGCAAATAAGGTGAGAAGGGCGGTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTgcggcg                                                                                                                                                                                                                                         >  1:552884/1‑251 (MQ=255)       aaaGTGCGGTCGATTTTCCCGGTGTTTGGCAAATAAGGTGAGAAGGGCGGTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGAtt                                                                                                                                                                                                                                   <  1:615724/251‑1 (MQ=255)                   aTTTTCCCGGTGTTTGGCAAATAAGGTGAGAAGGGCGGTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTAt                                                                                                                                                                                                                       >  1:391221/1‑251 (MQ=255)                                    cAAATAAGGTGAGAAGGGCGGTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGggt                                                                                                                                                                                                      <  1:739198/251‑1 (MQ=255)                                           ggTGAGAAGGGCGGTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTg                                                                                                                                                                                                >  1:227961/1‑250 (MQ=255)                                                        gTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGa                                                                                                                                                                                  >  1:724590/1‑251 (MQ=255)                                                             gTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAAtt                                                                                                                                                                                                                            <  1:38115/204‑1 (MQ=255)                                                                      aCCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCAtg                                                                                                                                                                    >  1:235294/1‑251 (MQ=255)                                                                         gCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATgtgt                                                                                                                                                                 >  1:361962/1‑251 (MQ=255)                                                                                     gagaAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTc                                                                                                                                                      >  1:885773/1‑250 (MQ=255)                                                                                     gagaAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTc                                                                                                                                                      >  1:665765/1‑250 (MQ=255)                                                                                                                ccGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGggt                                                                                                                                                                                                      >  1:523705/1‑175 (MQ=255)                                                                                                                ccGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTg                                                                                                                          >  1:476266/1‑251 (MQ=255)                                                                                                                  gTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTgg                                                                                                                         <  1:379531/250‑1 (MQ=255)                                                                                                                             tgtgAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAg                                                                                                              >  1:561005/1‑250 (MQ=255)                                                                                                                               tgAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGcc                                                                                                            <  1:353800/250‑1 (MQ=255)                                                                                                                               tgAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGcc                                                                                                            <  1:268772/250‑1 (MQ=255)                                                                                                                               tgAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGcc                                                                                                            <  1:277822/250‑1 (MQ=255)                                                                                                                                              aaGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCAccc                                                                                            <  1:588654/251‑1 (MQ=255)                                                                                                                                                gCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCa                                                                                           >  1:591660/1‑250 (MQ=255)                                                                                                                                                   tAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTCCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGcc                                                                                       <  1:66616/251‑1 (MQ=255)                                                                                                                                                          tAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAAtt                                                                                                                           <  1:736655/208‑1 (MQ=255)                                                                                                                                                          tAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGt                                                                                >  1:258782/1‑251 (MQ=255)                                                                                                                                                            aCATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGAc                                                                                                                                                                                 >  1:302398/1‑152 (MQ=255)                                                                                                                                                            aCATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGcc                                                                                       <  1:406613/242‑1 (MQ=255)                                                                                                                                                            aCATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGtt                                                                               >  1:447429/1‑250 (MQ=255)                                                                                                                                                            aCATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGtt                                                                               >  1:400625/1‑250 (MQ=255)                                                                                                                                                             cATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCa                                                                                                                                                                      <  1:527992/162‑1 (MQ=255)                                                                                                                                                             cATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCg                                                                             >  1:81115/1‑251 (MQ=255)                                                                                                                                                                 gCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGt                                                                         >  1:765309/1‑251 (MQ=255)                                                                                                                                                                 gCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAAAGGGTTCGTggg                                                                         >  1:238770/1‑250 (MQ=255)                                                                                                                                                                  ccTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCC‑TGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGt                                                                         <  1:366857/249‑1 (MQ=255)                                                                                                                                                                             gCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTa                                                             >  1:351363/1‑251 (MQ=255)                                                                                                                                                                             gCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACCCCCACGGGTTCGTGGTCGAAAGGATGTa                                                             >  1:376261/1‑251 (MQ=255)                                                                                                                                                                              cGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGt                                                              <  1:826769/249‑1 (MQ=255)                                                                                                                                                                                 gaggaAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATg                                                          <  1:519960/250‑1 (MQ=255)                                                                                                                                                                                 gaggaAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATCTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATg                                                          <  1:454610/250‑1 (MQ=255)                                                                                                                                                                                  aggaAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATgg                                                         <  1:457066/250‑1 (MQ=255)                                                                                                                                                                                  aggaAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATgg                                                         <  1:843659/250‑1 (MQ=255)                                                                                                                                                                                  aggaAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATgg                                                         <  1:813892/250‑1 (MQ=255)                                                                                                                                                                                  aggaAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTGGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTAAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATgg                                                         <  1:455435/250‑1 (MQ=255)                                                                                                                                                                                   ggaAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGa                                                        >  1:86312/1‑250 (MQ=255)                                                                                                                                                                                    gaAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGAc                                                       <  1:725914/250‑1 (MQ=255)                                                                                                                                                                                             gATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGcc                                                                                       >  1:56851/1‑209 (MQ=255)                                                                                                                                                                                              aTTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATaaatgc                                            <  1:593426/251‑1 (MQ=255)                                                                                                                                                                                              aTTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATaaatgc                                            <  1:7770/251‑1 (MQ=255)                                                                                                                                                                                                     gATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATaaatgccaaatg                                      >  1:868103/1‑250 (MQ=255)                                                                                                                                                                                                      aTTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATaaatgccaaatgc                                     >  1:856051/1‑250 (MQ=255)                                                                                                                                                                                                       ttttttGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGccc                                   <  1:73451/251‑1 (MQ=255)                                                                                                                                                                                                       ttttttGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGACGTAATGGACGGGATaaatgcccaatgcc                                    >  1:894945/1‑250 (MQ=255)                                                                                                                                                                                                            tgtgCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTc                              <  1:684541/251‑1 (MQ=255)                                                                                                                                                                                                            tgtgCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTc                              <  1:630678/251‑1 (MQ=255)                                                                                                                                                                                                            tgtgCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTc                              <  1:742193/251‑1 (MQ=255)                                                                                                                                                                                                               gCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGt                                                                            <  1:95489/202‑1 (MQ=255)                                                                                                                                                                                                               gCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCCAATGCCCTGTTcggg                           >  1:526136/1‑248 (MQ=255)                                                                                                                                                                                                               gCTTTAGATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTaa                                                            <  1:59037/218‑1 (MQ=255)                                                                                                                                                                                                                  ttATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATg                                                          >  1:755129/1‑217 (MQ=255)                                                                                                                                                                                                                  ttATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATaaatgc                                            >  1:295158/1‑231 (MQ=255)                                                                                                                                                                                                                  ttATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTag                         >  1:566201/1‑250 (MQ=255)                                                                                                                                                                                                                  ttATATTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTag                         >  1:333987/1‑250 (MQ=255)                                                                                                                                                                                                                   tataTTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATgg                                                         <  1:412405/217‑1 (MQ=255)                                                                                                                                                                                                                    atatTGGCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTagcaa                      >  1:671149/1‑251 (MQ=255)                                                                                                                                                                                                                         ggCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTAgcaagcaa                  >  1:352090/1‑250 (MQ=255)                                                                                                                                                                                                                          gCTGCGCGGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTAGCAAGCAAc                 <  1:143557/250‑1 (MQ=255)                                                                                                                                                                                                                              cgcgGGTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATaaatgc                                            >  1:822589/1‑219 (MQ=255)                                                                                                                                                                                                                                  ggTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATg                                                          <  1:677698/201‑1 (MQ=255)                                                                                                                                                                                                                                  ggTTGATGATAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATaaatg                                             <  1:589408/214‑1 (MQ=255)                                                                                                                                                                                                                                      gatgatAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTaa                                                            <  1:419721/195‑1 (MQ=255)                                                                                                                                                                                                                                        tgatAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTag                         <  1:326938/228‑1 (MQ=255)                                                                                                                                                                                                                                        tgatAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTAGCAAGCAACATTTCAAat        <  1:609851/245‑1 (MQ=255)                                                                                                                                                                                                                                        tgatAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACAGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTAGCAAGCAACATTTCAAattgattg  >  1:435992/1‑251 (MQ=255)                                                                                                                                                                                                                                         gatAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATg                                                          >  1:668569/1‑194 (MQ=255)                                                                                                                                                                                                                                          atAAGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCGGTag                         <  1:97601/226‑1 (MQ=255)                                                                                                                                                                                                                                            aaGATCGCGTGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTg                                                                                                                                                                                   <  1:861578/70‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              CAACTGAAAGTGCGGTCGATTTTCCCGGTGTTTGGCAAATAAGGTGAGAAGGGCGGTAGAGTCCAAGCCACCGCTAAATCCAATGAGAAATTGCGTTTGATTTGGTGCGTGCCGTTGTAACTGCTGTGAGAATTGTTCGAGAAGCATAGCGGATAACATAGCCTCCCGCTAAGCGGGAGGAAGTGGCAGATTAAGCGATTTTTTGTGCTTTATATTGGCTGCGCGGGTTGATGATAAGATCGCGGGCGGCGATGATTTGTAATTCGTATTCGCCACGTTCCAGGGTGGTTTGCATCACATCGTTGACTGCGTTTGCCATGTGTTCAAAAGCCTCCACATCGGATTTGCCGTTGAGTAAATTGGCTAAGAATAAGCCGGCGGTTAAATCACCCACGCCAACGGGTTCGTGGTCGAACGGATGTAATGGACGGGATAAATGCCAAATGCCCTGTTCAGTAGCAAGCAACATTTCAAATTGATTG  >  1/1280053‑1280534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑