Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,279,819	A→G	T127T (ACT→ACC)	unknown ←	tRNA(Ile)‑lysidine synthetase (EC 6.3.4.19)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,279,819	0	A	G	100.0%	193.1 / NA	66	T127T (ACT→ACC)	unknown	tRNA(Ile)‑lysidine synthetase (EC 6.3.4.19)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/41);  total (25/41)

CGCCCAGCGTTGGCGAAATTCAGGCAACACCACATTGCGCAGAAAATTGCGGTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAAGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGGTGAATGTGAATAGCGCGCAACTGAAAGT  >  1/1279579‑1280063                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     cgcCCAGCGTTGGCGAAATTCAGGCAACACCACATTGCGCAGAAAATTGCGGTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGttt                                                                                                                                                                                                                                            >  1:736795/1‑251 (MQ=255)                aaaTTCAGGCAACACCACATTGCGCAGAAAATTGCGGTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATgg                                                                                                                                                                                                                              <  1:671056/250‑1 (MQ=255)                    tCAGGCAACACCACATTGCGCAGAAAATTGCGGTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTgcgc                                                                                                                                                                                                                         <  1:892790/251‑1 (MQ=255)                                      cgcAGAAAATTGCGGTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCAttctt                                                                                                                                                                                                        <  1:91676/250‑1 (MQ=255)                                           aaaaTTGCGGTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCggg                                                                                                                                                                                                    <  1:56860/249‑1 (MQ=255)                                                    gTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATAtgtt                                                                                                                                                                                         >  1:713076/1‑251 (MQ=255)                                                    gTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATAtgt                                                                                                                                                                                          <  1:577534/250‑1 (MQ=255)                                                    gTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATAtgt                                                                                                                                                                                          <  1:551626/250‑1 (MQ=255)                                                        tATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGc                                                                                                                                                                                     <  1:484398/251‑1 (MQ=255)                                                              ttgTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGc                                                                                                                                                                               <  1:620710/251‑1 (MQ=255)                                                                     cGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATaa                                                                                                                                                                        <  1:598260/251‑1 (MQ=255)                                                                     cGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATaa                                                                                                                                                                        <  1:578609/251‑1 (MQ=255)                                                                     cGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATaa                                                                                                                                                                        <  1:511163/251‑1 (MQ=255)                                                                       ttACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAAc                                                                                                                                                                       <  1:138479/250‑1 (MQ=255)                                                                       ttACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAAc                                                                                                                                                                       <  1:34119/250‑1 (MQ=255)                                                                       ttACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACg                                                                                                                                                                      <  1:234875/251‑1 (MQ=255)                                                                         aCTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATgg                                                                                                                                                                                >  1:691110/1‑239 (MQ=255)                                                                                   tCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGc                                                                                                                                                          <  1:708553/251‑1 (MQ=255)                                                                                                gctgttgctgttGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATaa                                                                                                                                                                        >  1:368216/1‑224 (MQ=255)                                                                                                 ctgttgctgttgCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGc                                                                                                                                            <  1:115051/251‑1 (MQ=255)                                                                                                   gttgctgttgcACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCg                                                                                                                                          >  1:581414/1‑251 (MQ=255)                                                                                                    ttgctgttgcACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGt                                                                                                                                         <  1:871458/251‑1 (MQ=255)                                                                                                       ctgttgcACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTg                                                                                                                                                                                                                                  <  1:548053/159‑1 (MQ=255)                                                                                                       ctgttgcACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCAttctt                                                                                                                                                                                                        >  1:341950/1‑185 (MQ=255)                                                                                                                 aaTGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCa                                                                                                                             >  1:667402/1‑250 (MQ=255)                                                                                                                       aTCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAAc                                                                                                                      <  1:140881/251‑1 (MQ=255)                                                                                                                              gCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGa                                                                                                               >  1:632863/1‑251 (MQ=255)                                                                                                                                      cTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCa                                                                                                        <  1:189273/250‑1 (MQ=255)                                                                                                                                         aaaTTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATc                                                                                                            <  1:863813/243‑1 (MQ=255)                                                                                                                                           aTTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGaa                                                                                                  <  1:621361/251‑1 (MQ=255)                                                                                                                                           aTTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGACGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGaa                                                                                                  >  1:772979/1‑251 (MQ=255)                                                                                                                                                      ggCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTg                                                                                        <  1:845346/250‑1 (MQ=255)                                                                                                                                                               tCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAatat                                                                                               >  1:414849/1‑234 (MQ=255)                                                                                                                                                                       ccGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCAttcttc                                                                                                                                                                                                       <  1:404199/122‑1 (MQ=255)                                                                                                                                                                       ccGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGc                                                                                   <  1:615280/238‑1 (MQ=255)                                                                                                                                                                       ccGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCa                                                                      >  1:737559/1‑251 (MQ=255)                                                                                                                                                                        cGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCAGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCaa                                                                     >  1:429338/1‑251 (MQ=255)                                                                                                                                                                                aTGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGt                                                             >  1:827578/1‑251 (MQ=255)                                                                                                                                                                                   cTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATaa                                                                                                                                                                        >  1:69923/1‑141 (MQ=255)                                                                                                                                                                                   cTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGc                                                                <  1:590365/245‑1 (MQ=255)                                                                                                                                                                                       tAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCa                                                      <  1:361765/251‑1 (MQ=255)                                                                                                                                                                                       tAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCa                                                      <  1:297396/251‑1 (MQ=255)                                                                                                                                                                                             gcattgcaCCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCAt                                                                                                                                                                          <  1:791450/129‑1 (MQ=255)                                                                                                                                                                                             gcattgcaCCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTg                                                                                        >  1:337088/1‑211 (MQ=255)                                                                                                                                                                                             gcattgcaCCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCa                                                >  1:112259/1‑251 (MQ=255)                                                                                                                                                                                             gcattgcaCCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCa                                                >  1:413192/1‑251 (MQ=255)                                                                                                                                                                                                 tgcaCCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAg                                            <  1:672422/251‑1 (MQ=255)                                                                                                                                                                                                  gcaCCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGc                                                                       >  1:293251/1‑223 (MQ=255)                                                                                                                                                                                                  gcaCCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAgg                                           >  1:377558/1‑251 (MQ=255)                                                                                                                                                                                                    cccTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGc                                         <  1:743486/250‑1 (MQ=255)                                                                                                                                                                                                    aCCTAACCCGTACAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGc                                         <  1:294865/251‑1 (MQ=255)                                                                                                                                                                                                    aCCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAggg                                          >  1:385262/1‑250 (MQ=255)                                                                                                                                                                                                             cTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCAt                                >  1:198374/1‑251 (MQ=255)                                                                                                                                                                                                                cAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATgg                              <  1:732467/250‑1 (MQ=255)                                                                                                                                                                                                                 aaaCCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtg                            <  1:569425/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaCCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtg                            <  1:413698/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaCCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtg                            <  1:420971/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaCCGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtg                            <  1:575324/251‑1 (MQ=255)                                                                                                                                                                                                                    ccGCTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtgaat                         >  1:469192/1‑251 (MQ=255)                                                                                                                                                                                                                       cTACCGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtgaatgt                       <  1:752320/250‑1 (MQ=255)                                                                                                                                                                                                                          ccGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtgaatgtgaat                   <  1:668819/251‑1 (MQ=255)                                                                                                                                                                                                                          ccGCGTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtgaatgtgaat                   <  1:7374/251‑1 (MQ=255)                                                                                                                                                                                                                             cgTTTGAGCGCAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGGTGAATGTGAATAg                 <  1:834139/250‑1 (MQ=255)                                                                                                                                                                                                                                    gcgcAAGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTg                                                                                        >  1:461615/1‑172 (MQ=255)                                                                                                                                                                                                                                         aGAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGgtgaatgtgaat                   >  1:872845/1‑236 (MQ=255)                                                                                                                                                                                                                                          gAAAAAAGGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACAGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGGTGAATGTGAATAg                 >  1:179547/1‑237 (MQ=255)                                                                                                                                                                                                                                           aaaaaagGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGGTGAATGTGAATAGCGCGCAACTGAAAGt  <  1:728631/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     CGCCCAGCGTTGGCGAAATTCAGGCAACACCACATTGCGCAGAAAATTGCGGTCGTATTGATTGTCTTCGTTACTTTCGTCTTCAACCCAAGTCAGCTGTTGCTGTTGCACGAATGCCATCAGTTGCTGACGGCTAAAATTCAATAGCGGGCGAAAAATCGGTACGCCGTAAACGATGCTGTTAGCTTGCATTGCACCTAACCCCTGCAAACCGCTACCGCGTTTGAGCGCAAGAAAAAAAGTTTCCGTTTGATCTTGCTGATGGTGCGCCGTGGCTAGCCATTCTTCGGGCAAAATATGTTGTGCGATGGCTTCATAACGTGCCTCACGGGCACCGGCTTCAATGCCGTTTTGCAAACGCACTTTAACCGGCTGAATCAGCAACGGAATATTCAATTGTCGGCAAATTTGTTGGCAATGTGCCGTCCAGGCATCGGCATTAGGGCTTAAGCCATGGTGAATGTGAATAGCGCGCAACTGAAAGT  >  1/1279579‑1280063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑