Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,249,186	T→C	intergenic (‑330/‑27)	unknown ← / → unknown	Nucleoside‑diphosphate‑sugar epimerases/Peptide chain release factor 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,249,186	0	T	C	100.0%	224.8 / NA	74	intergenic (‑330/‑27)	unknown/unknown	Nucleoside‑diphosphate‑sugar epimerases/Peptide chain release factor 3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/37);  total (37/37)

AACGCTTCTTGATATTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTTTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAAACAACGCGGGATTTCCATTACCACCTCCGTCATGC  >  1/1248939‑1249432                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aaCGCTTCTTGATATTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTttcttc                                                                                                                                                                                                                                                     <  1:382004/251‑1 (MQ=255) aaCGCTTCTTGATATTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTttcttc                                                                                                                                                                                                                                                     <  1:638977/251‑1 (MQ=255)     cttcttGATATTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCatta                                                                                                                                                                                                                                                 >  1:50973/1‑251 (MQ=255)      ttcttGATATTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCattat                                                                                                                                                                                                                                                <  1:16239/251‑1 (MQ=255)      ttcttGATATTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCattat                                                                                                                                                                                                                                                <  1:826847/251‑1 (MQ=255)        cttGATATTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCAttattt                                                                                                                                                                                                                                              >  1:487326/1‑251 (MQ=255)             tatTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAg                                                                                                                                                                                                                                         <  1:779164/251‑1 (MQ=255)               tttCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGAc                                                                                                                                                                                                                                       <  1:760967/251‑1 (MQ=255)                 tCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTttcttc                                                                                                                                                                                                                                                     <  1:866771/235‑1 (MQ=255)                          aCTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGAt                                                                                                                                                                                                                            >  1:281862/1‑251 (MQ=255)                          aCTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGAt                                                                                                                                                                                                                            >  1:42437/1‑251 (MQ=255)                             ttGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGac                                                                                                                                                                                                                         <  1:100082/251‑1 (MQ=255)                                           tCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGaa                                                                                                                                                                                                            <  1:838620/250‑1 (MQ=255)                                           tCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAAc                                                                                                                                                                                                           <  1:617819/251‑1 (MQ=255)                                           tCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAAc                                                                                                                                                                                                           <  1:879706/251‑1 (MQ=255)                                                   gATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAAcagcag                                                                                                                                                                                                                              >  1:824546/1‑224 (MQ=255)                                                        ttttAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAAc                                                                                                                                                                                                           >  1:194279/1‑238 (MQ=255)                                                                  gAGTTGTGTCATTTCATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAg                                                                                                                                                                                                                                         >  1:102333/1‑198 (MQ=255)                                                                             ttttATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTtc                                                                                                                                                                         <  1:51577/251‑1 (MQ=255)                                                                               ttattaCCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCaa                                                                                                                                                                                                 >  1:430114/1‑225 (MQ=255)                                                                                 attaCCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCAc                                                                                                                                                                     <  1:420055/251‑1 (MQ=255)                                                                                 attaCCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCAc                                                                                                                                                                     <  1:479299/251‑1 (MQ=255)                                                                                   taCCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCAccc                                                                                                                                                                   <  1:134309/251‑1 (MQ=255)                                                                                     ccTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTttcttc                                                                                                                                                                                                                                                     <  1:708456/167‑1 (MQ=255)                                                                                     ccTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAAc                                                                                                                                                                                                           >  1:340736/1‑209 (MQ=255)                                                                                     ccTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCg                                                                                                                                                                 >  1:324513/1‑251 (MQ=255)                                                                                                ggCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGc                                                                                                                                                             >  1:415057/1‑244 (MQ=255)                                                                                                              cgcgTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGc                                                                                                                                                             >  1:380003/1‑230 (MQ=255)                                                                                                                 gTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGAc                                                                                                                                                                                                                                       <  1:593948/153‑1 (MQ=255)                                                                                                                 gTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAacc                                                                                                                                                   <  1:306287/237‑1 (MQ=255)                                                                                                                 gTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGaaaa                                                                                                                                     >  1:880405/1‑251 (MQ=255)                                                                                                                 gTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGaaaa                                                                                                                                     >  1:874601/1‑251 (MQ=255)                                                                                                                    ttGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTttcttc                                                                                                                                                                                                                                                     >  1:6568/1‑136 (MQ=255)                                                                                                                    ttGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATc                                                                                                                                                                                                                    >  1:692967/1‑169 (MQ=255)                                                                                                                      gTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAgtgt                                                                                                                                >  1:302376/1‑251 (MQ=255)                                                                                                                            cACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCtt                                                                                                                           <  1:713671/250‑1 (MQ=255)                                                                                                                            cACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAATCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGac                                                                                                                                                                                                                         <  1:738092/156‑1 (MQ=255)                                                                                                                                ttttACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACgg                                                                                                                      <  1:493017/251‑1 (MQ=255)                                                                                                                                ttttACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACgg                                                                                                                      <  1:709131/251‑1 (MQ=255)                                                                                                                                    aCGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGat                                                                                                                                                                               >  1:872336/1‑190 (MQ=255)                                                                                                                                       tAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGc                                                                                                                <  1:843666/250‑1 (MQ=255)                                                                                                                                                                ttGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAg                                                                                      >  1:153293/1‑251 (MQ=255)                                                                                                                                                                   ttAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGc                                                                                    <  1:714025/250‑1 (MQ=255)                                                                                                                                                                    tAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCa                                                                                                                                                                                         >  1:664539/1‑148 (MQ=255)                                                                                                                                                                         ttattTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACg                                                                                                                                                                                       <  1:697177/145‑1 (MQ=255)                                                                                                                                                                         ttattTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGc                                                                                                                     <  1:705119/211‑1 (MQ=255)                                                                                                                                                                         ttattTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGtt                                                                             >  1:260218/1‑251 (MQ=255)                                                                                                                                                                         ttattTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGtt                                                                             >  1:163612/1‑251 (MQ=255)                                                                                                                                                                            ttttCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCgg                                                                          >  1:111917/1‑251 (MQ=255)                                                                                                                                                                             tttCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCgg                                                                          >  1:614896/1‑250 (MQ=255)                                                                                                                                                                                   gTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCaaa                                                                                                        <  1:883710/214‑1 (MQ=255)                                                                                                                                                                                   gTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAaca                                                                   >  1:770810/1‑251 (MQ=255)                                                                                                                                                                                     cTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACg                                                                 >  1:97423/1‑251 (MQ=255)                                                                                                                                                                                     cTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACg                                                                 >  1:328587/1‑251 (MQ=255)                                                                                                                                                                                                 ggCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGc                                                                                    <  1:92384/220‑1 (MQ=255)                                                                                                                                                                                                 ggCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGgc                                                                         >  1:123335/1‑231 (MQ=255)                                                                                                                                                                                                  gCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGt                                                                                                                                                         >  1:513266/1‑150 (MQ=255)                                                                                                                                                                                                  gCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAacc                                                                                                                                                   >  1:539917/1‑156 (MQ=255)                                                                                                                                                                                                  gCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATc                                                          <  1:116726/245‑1 (MQ=255)                                                                                                                                                                                                  gCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGa                                                       >  1:30849/1‑248 (MQ=255)                                                                                                                                                                                                  gCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGa                                                       >  1:327959/1‑248 (MQ=255)                                                                                                                                                                                                   cttaaaaGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAAc                                                                                                                                                                                                           <  1:273810/96‑1 (MQ=255)                                                                                                                                                                                                     aaaaaGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTg                                                                                                                                                                                                      >  1:7219/1‑102 (MQ=255)                                                                                                                                                                                                       aaaGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGAc                                                                                                                                                                                                                                       <  1:204824/67‑1 (MQ=255)                                                                                                                                                                                                          gCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACt                                                     <  1:105526/242‑1 (MQ=255)                                                                                                                                                                                                          gCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGaaa                                            >  1:27916/1‑251 (MQ=255)                                                                                                                                                                                                          gCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGaaa                                            >  1:12052/1‑251 (MQ=255)                                                                                                                                                                                                            atatAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGc                                                                                                                     <  1:896088/176‑1 (MQ=255)                                                                                                                                                                                                               taGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGgag                                       <  1:717542/251‑1 (MQ=255)                                                                                                                                                                                                               taGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGgag                                       <  1:858543/251‑1 (MQ=255)                                                                                                                                                                                                               taGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGgag                                       <  1:617056/251‑1 (MQ=255)                                                                                                                                                                                                                   tGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAaac                                   <  1:772375/251‑1 (MQ=255)                                                                                                                                                                                                                   tGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAaac                                   <  1:362145/251‑1 (MQ=255)                                                                                                                                                                                                                     cgcgACCAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAaacaa                                 <  1:545560/251‑1 (MQ=255)                                                                                                                                                                                                                           cAAATTCTCAGCCGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAAACAACGCggg                           <  1:394142/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAAACAACGCGGGATTTCCATTacc               <  1:323047/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGTCTCAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAAACAACGCGGGATTTCCATTac                >  1:527939/1‑250 (MQ=255)                                                                                                                                                                                                                                         tctcAGGACGGTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAAACAACGCGGGATTTCCATTacca              >  1:709961/1‑250 (MQ=255)                                                                                                                                                                                                                                                   gTTTTCTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAAACAACGCGGGATTTCCATTACCACCTCCGTCATg   >  1:505403/1‑251 (MQ=255)                                                                                                                                                                                                                                                    ttttcttCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAAACAACGCGGGATTTCCATTACCACCTCCGTCATGc  <  1:412625/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       AACGCTTCTTGATATTTCTGCAATTACTTTGGCGACACTTTGTCGGAATAGATTTTTTTAAGACTGAGTTGTGTCATTTTATTACCTTTTATCCGGGCAAAAATCATGGCGCGTATTGTACCGCACTTTTTACGTAAACGTTTGCATTTTTTGCGGTAATTGTTAGCATTATTTTCCAGTCTTGAAAAGACAGGCTAAAAAGCATATAGTTGCGCGACCAAATTCTCAGCCGTCTCAGGACGGTTTTTTTCATTATTTATAAGACAAACAGCAGATGACACATCCTCAAGAACAAGTGAGCAAACGTCGCACGTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATTACCGAAAAAGTGTTGCTTTACGGCAACGCCATTCAAAAAGCCGGTTCGGTGAAAGGCAAGGGTTCGGCGCAACACGCCAAATCCGACTGGATGGAAATGGAGAAACAACGCGGGATTTCCATTACCACCTCCGTCATGC  >  1/1248939‑1249432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑