Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,248,147	G→A	P237L (CCG→CTG)	unknown ←	Nucleoside‑diphosphate‑sugar epimerases

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,248,147	0	G	A	100.0%	208.4 / NA	72	P237L (CCG→CTG)	unknown	Nucleoside‑diphosphate‑sugar epimerases
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/37);  total (35/37)

TTCGGTAAGTGTTGCAAAATGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTACAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCGGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATCTTGCCCCACAAGACCGGCAAAACGAATAA  >  1/1247903‑1248390                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ttCGGTAAGTGTTGCAAAATGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGAt                                                                                                                                                                                                                                               >  1:535935/1‑251 (MQ=255)      tAAGTGTTGCAAAATGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTAc                                                                                                                                                                                                                                           <  1:758043/250‑1 (MQ=255)         gtgtTGCAAAATGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACa                                                                                                                                                                                                                                          >  1:700064/1‑248 (MQ=255)         gtgtTGCAAAATGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATgg                                                                                                                                                                                                                                       >  1:623125/1‑251 (MQ=255)           gtTGCAAAATGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGgtg                                                                                                                                                                                                                                     >  1:734191/1‑251 (MQ=255)            ttGCAAAATGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGgtgt                                                                                                                                                                                                                                    <  1:659127/251‑1 (MQ=255)                 aaaTGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTc                                                                                                                                                                                                                               <  1:840087/251‑1 (MQ=255)                   aTGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACAt                                                                                                                                                                                                                                         >  1:714118/1‑239 (MQ=255)                             aCATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCg                                                                                                                                                                                                                   >  1:366387/1‑251 (MQ=255)                                 atTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACa                                                                                                                                                                                                                                          >  1:583515/1‑224 (MQ=255)                                      ctttcCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGc                                                                                                                                                                                                          <  1:404228/251‑1 (MQ=255)                                      ctttcCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGc                                                                                                                                                                                                          <  1:360828/251‑1 (MQ=255)                                          ccTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTcg                                                                                                                                                                                                      >  1:531845/1‑251 (MQ=255)                                                  gttttgtGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCgg                                                                                                                                                                                                                  >  1:593355/1‑231 (MQ=255)                                                       gtgtTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCa                                                                                                                                                                                         >  1:409644/1‑251 (MQ=255)                                                             aTTTTGGGTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAg                                                                                                                                                                                                            <  1:191867/226‑1 (MQ=255)                                                                   ggTTAAACGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCa                                                                                                                                                                             >  1:555917/1‑251 (MQ=255)                                                                        aaCGAGTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGa                                                                                                                                                                        <  1:327617/251‑1 (MQ=255)                                                                             gTTTTAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTAc                                                                                                                                                                                                                                           <  1:521723/179‑1 (MQ=255)                                                                              ttttAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAgg                                                                                                                                                                  <  1:795212/251‑1 (MQ=255)                                                                              ttttAATAAAAAGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAg                                                                                                                                                                   <  1:333641/250‑1 (MQ=255)                                                                                     aaaaaGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGc                                                                                                                                                           <  1:582530/251‑1 (MQ=255)                                                                                     aaaaaGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGc                                                                                                                                                           <  1:630298/251‑1 (MQ=255)                                                                                      aaaaGTGCAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGcc                                                                                                                                                          <  1:864785/251‑1 (MQ=255)                                                                                              aaCTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGgtg                                                                                                                                                  <  1:788873/251‑1 (MQ=255)                                                                                                           cTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATg                                                                                                                                                                                           >  1:352306/1‑197 (MQ=255)                                                                                                                 aaCCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAAt                                                                                                                               <  1:648145/251‑1 (MQ=255)                                                                                                                 aaCCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTGTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAAt                                                                                                                               <  1:441105/251‑1 (MQ=255)                                                                                                                   ccgaccgCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATgg                                                                                                                             <  1:438178/251‑1 (MQ=255)                                                                                                                          cACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGc                                                                                                                                                                                                          >  1:577447/1‑167 (MQ=255)                                                                                                                          cACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATAc                                                                                                                                                                     <  1:6932/204‑1 (MQ=255)                                                                                                                           aCTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGgcg                                                                                                                     <  1:486653/251‑1 (MQ=255)                                                                                                                            cTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTg                                                                                                                                  <  1:237284/237‑1 (MQ=255)                                                                                                                                    cttcCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATc                                                                                                             >  1:767933/1‑250 (MQ=255)                                                                                                                                                  aGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAg                                                                                                                                     <  1:875640/212‑1 (MQ=255)                                                                                                                                                   gATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATAc                                                                                                                                                                     >  1:287765/1‑179 (MQ=255)                                                                                                                                                   gATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTg                                                                                                                                  >  1:289306/1‑214 (MQ=255)                                                                                                                                                    aTTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAAtt                                                                                              <  1:394503/249‑1 (MQ=255)                                                                                                                                                       tGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCg                                                                                         >  1:465539/1‑251 (MQ=255)                                                                                                                                                             ggggTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCAc                                                                                           >  1:736985/1‑243 (MQ=255)                                                                                                                                                              gggTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCTGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATgg                                                                                                                             >  1:869568/1‑208 (MQ=255)                                                                                                                                                              gggTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACaa                                                                                                                                                                   >  1:425563/1‑169 (MQ=255)                                                                                                                                                              gggTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGcc                                                                                                                                                          >  1:364089/1‑179 (MQ=255)                                                                                                                                                              gggTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGt                                                                                                                 >  1:475243/1‑220 (MQ=255)                                                                                                                                                              gggTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGCGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACg                                                                                  >  1:531443/1‑251 (MQ=255)                                                                                                                                                                 tATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGAc                                                                                <  1:307761/250‑1 (MQ=255)                                                                                                                                                                     gATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGa                                                                                                                                                                        <  1:532726/158‑1 (MQ=255)                                                                                                                                                                     gATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCa                                                                            >  1:749700/1‑250 (MQ=255)                                                                                                                                                                          aCGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAAt                                                                                                        <  1:827608/217‑1 (MQ=255)                                                                                                                                                                           cGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTgaga                                                                     <  1:799366/251‑1 (MQ=255)                                                                                                                                                                             aaaTCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATc                                                                   <  1:837408/251‑1 (MQ=255)                                                                                                                                                                              aaTCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATcc                                                                  <  1:184744/251‑1 (MQ=255)                                                                                                                                                                               aTCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTgag                                                                      >  1:309028/1‑246 (MQ=255)                                                                                                                                                                                               aTTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTcc                                                                                                                                             >  1:57186/1‑159 (MQ=255)                                                                                                                                                                                                         ccACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGa                                       >  1:199183/1‑251 (MQ=255)                                                                                                                                                                                                            caATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATgg                                    >  1:369588/1‑251 (MQ=255)                                                                                                                                                                                                                      cTGTGGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAGAAATCGGATGGCGATTTTGc                           >  1:470489/1‑250 (MQ=255)                                                                                                                                                                                                                       tgtgGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGccc                         <  1:776893/251‑1 (MQ=255)                                                                                                                                                                                                                        gtgGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTcc                                                                                                                                             <  1:487805/134‑1 (MQ=255)                                                                                                                                                                                                                        gtgGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCgg                                                        <  1:660721/219‑1 (MQ=255)                                                                                                                                                                                                                        gtgGATCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGccc                         >  1:390105/1‑250 (MQ=255)                                                                                                                                                                                                                            aTCTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAGTAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGACTGGCGCGGGCGCAGTCATCCACATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGGCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACaa                    >  1:835150/1‑251 (MQ=255)                                                                                                                                                                                                                              cTTCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGc                                                                                                                                                                                                          <  1:200550/67‑1 (MQ=255)                                                                                                                                                                                                                               ttCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACAAGAc                 <  1:647141/251‑1 (MQ=255)                                                                                                                                                                                                                               ttCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACAAGAc                 <  1:808512/251‑1 (MQ=255)                                                                                                                                                                                                                               ttCCGGTGCGCTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACAAGAc                 <  1:416480/251‑1 (MQ=255)                                                                                                                                                                                                                                      gcgcTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACAAGACCGGCaaa          >  1:50689/1‑251 (MQ=255)                                                                                                                                                                                                                                         cTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACAAGACCGGCaaacg        <  1:721704/250‑3 (MQ=255)                                                                                                                                                                                                                                         cTGATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACAAGACCGGCAAAACg       >  1:68531/1‑251 (MQ=255)                                                                                                                                                                                                                                           gATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTc                                                          <  1:17103/198‑1 (MQ=255)                                                                                                                                                                                                                                           gATAAAGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACAAGACCGGCAAAACGaa     >  1:384377/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaaGTGCAGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATTTTGCCCCACAAGACCGGCAAAACGAATaa  >  1:386746/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TTCGGTAAGTGTTGCAAAATGGTATTTGACATATTTTCTTTCCTTATTTGTTTTGTGTTTATTTTGGGTTAAACGAGTTTTAATAAAAAGTACAACTAAAAAATAACTTAAAAACCGACCGCACTTTTATTCTTCCGAAAACATCAGATTTGGATCGGGGTATTGATAAACGAAATCCAGTTCACGACAAATTTTGTTCGCCACAATAATGCGCTGTGGATCTTCCGGTGCGCTGATAAAGTGCGGTGGATTTACATGGTGTTTTTCCGCTGCCAAGCGGTAATAGGCTTCGCGGGGCGGATGCACGGGCGCTGCCAAATGATACAGGCGATAGCCACCGGGTGTTTCCAGCAATAGTTGAATGGCGCGGGCGCAGTCATCCAAATGCACTAAATTCACCGGCGAACGACCGCACTTGAGATCCTGTTTGTCGGCGAGGGAAAAAATCGGATGGCGATCTTGCCCCACAAGACCGGCAAAACGAATAA  >  1/1247903‑1248390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑