Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,238,027	G→A	V273V (GTC→GTT)	unknown ←	Formate dehydrogenase formation protein FdhE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,238,027	0	G	A	100.0%	234.2 / NA	78	V273V (GTC→GTT)	unknown	Formate dehydrogenase formation protein FdhE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/45);  total (33/45)

GTATCCGATGAAACAGGTGGGCACCGATGAAGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATGCCTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATGACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTTTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGAATCATGCTGGCGGTCGGCGCAGAGC  >  1/1237782‑1238271                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gTATCCGATGAAACAGGTGGGCACCGATGAAGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTc                                                                                                                                                                                                                                                 >  1:363016/1‑250 (MQ=255)                ggTGGGCACCGATGAAGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCCTTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCttt                                                                                                                                                                                                                                  >  1:600894/1‑250 (MQ=255)                ggTGGGCACCGATGAAGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCttt                                                                                                                                                                                                                                  >  1:894398/1‑250 (MQ=255)                ggTGGGCACCGATGAAGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCttt                                                                                                                                                                                                                                  >  1:205231/1‑250 (MQ=255)                ggTGGGCACCGATGAAGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCttt                                                                                                                                                                                                                                  >  1:196947/1‑250 (MQ=255)                  tGGGCACCGATGAAGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTtctc                                                                                                                                                                                                                               <  1:611386/251‑1 (MQ=255)                              aaGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATagag                                                                                                                                                                                                                    <  1:697535/250‑1 (MQ=255)                              aaGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATagag                                                                                                                                                                                                                    >  1:155128/1‑250 (MQ=255)                              aaGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGc                                                                                                                                                                                                                   <  1:900211/251‑1 (MQ=255)                              aaGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGc                                                                                                                                                                                                                   <  1:850453/251‑1 (MQ=255)                                          ccGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCt                                                                                                                                                                                                       <  1:463520/251‑1 (MQ=255)                                          ccGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCt                                                                                                                                                                                                       <  1:858907/251‑1 (MQ=255)                                                 tCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAg                                                                                                                                                                                                <  1:874368/251‑1 (MQ=255)                                                        gATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATagag                                                                                                                                                                                                                    >  1:149327/1‑224 (MQ=255)                                                                 ggCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCa                                                                                                                                                                                 >  1:592351/1‑250 (MQ=255)                                                                  gCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCAc                                                                                                                                                                                <  1:196845/250‑1 (MQ=255)                                                                   cAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACt                                                                                                                                                                               <  1:245349/250‑1 (MQ=255)                                                                   cAAATAATCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACt                                                                                                                                                                               <  1:266605/250‑1 (MQ=255)                                                                         atCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGc                                                                                                                                                                                                                   <  1:412939/208‑1 (MQ=255)                                                                         atCCAAAAGCAAAAGGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGAt                                                                                                                                                                         >  1:772756/1‑250 (MQ=255)                                                                                agcaaaagGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCAt                                                                                                                                                                                                                                              <  1:513988/174‑1 (MQ=255)                                                                                agcaaaagGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGc                                                                                                                                                                                                  >  1:6971/1‑218 (MQ=255)                                                                                 gcaaaagGCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAg                                                                                                                                                                >  1:700817/1‑251 (MQ=255)                                                                                        gCATCCGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGc                                                                                                                                                                                      >  1:797048/1‑222 (MQ=255)                                                                                             cGTTGGATG‑CTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCaaa                                                                                                                                                    <  1:527954/251‑1 (MQ=255)                                                                                                   gatgcTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGAc                                                                                                                                                <  1:597224/246‑1 (MQ=255)                                                                                                      gcTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCaat                                                                                                                                            >  1:577667/2‑251 (MQ=255)                                                                                                       cTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAg                                                                                                                                                                                                <  1:786336/198‑1 (MQ=255)                                                                                                         tttttGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCaataat                                                                                                                                         >  1:783992/1‑251 (MQ=255)                                                                                                                                 ttttATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGt                                                                                                                 <  1:390472/251‑1 (MQ=255)                                                                                                                                 ttttATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGt                                                                                                                 <  1:753255/251‑1 (MQ=255)                                                                                                                                      tGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTg                                                                                                            <  1:416393/251‑1 (MQ=255)                                                                                                                                        aaTGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCa                                                                                                          <  1:571804/251‑1 (MQ=255)                                                                                                                                           gTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGAt                                                                                                                                                                         <  1:419664/185‑1 (MQ=255)                                                                                                                                           gTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTg                                                                                                       >  1:684957/1‑251 (MQ=255)                                                                                                                                               tttATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTg                                                                                                   <  1:255357/251‑1 (MQ=255)                                                                                                                                                ttattaTTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGt                                                                                                                 <  1:48084/236‑1 (MQ=255)                                                                                                                                                ttattaTTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTg                                                                                                       >  1:172508/1‑246 (MQ=255)                                                                                                                                                    tattCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCAc                                                                                              <  1:899804/251‑1 (MQ=255)                                                                                                                                                     attCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCAc                                                                                              >  1:592259/1‑250 (MQ=255)                                                                                                                                                          ggcggGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCaataa                                                                                                                                          >  1:194200/1‑201 (MQ=255)                                                                                                                                                            cggGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTcc                                                                                      <  1:175810/251‑1 (MQ=255)                                                                                                                                                               gAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATagag                                                                                                                                                                                                                    >  1:836427/1‑122 (MQ=255)                                                                                                                                                               gAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAg                                                                                                                                                                                                >  1:49463/1‑142 (MQ=255)                                                                                                                                                                          ggTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCaataa                                                                                                                                          <  1:16463/185‑1 (MQ=255)                                                                                                                                                                          ggTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGt                                                                                                                 <  1:846407/210‑1 (MQ=255)                                                                                                                                                                                  ccGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTCCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAg                                                                  <  1:446056/249‑1 (MQ=255)                                                                                                                                                                                    gCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCg                                                                                                                                                                            <  1:254565/141‑1 (MQ=255)                                                                                                                                                                                       tttCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCAt                                                                                           <  1:525828/219‑1 (MQ=255)                                                                                                                                                                                         tCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGTGCAGTGCAgg                                                         >  1:870853/1‑251 (MQ=255)                                                                                                                                                                                           aTAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTa                                                       <  1:68980/251‑1 (MQ=255)                                                                                                                                                                                           aTAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTa                                                       <  1:626766/251‑1 (MQ=255)                                                                                                                                                                                           aTAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTa                                                       <  1:11931/251‑1 (MQ=255)                                                                                                                                                                                           aTAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCCCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTa                                                       <  1:453956/251‑1 (MQ=255)                                                                                                                                                                                                gCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGCTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTACATTGGTTGCGCACCATGTTc                                                                                       >  1:607228/1‑214 (MQ=255)                                                                                                                                                                                                 ccTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCa                                                  <  1:243796/250‑1 (MQ=255)                                                                                                                                                                                                    tttttCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGcc                                              <  1:368051/251‑1 (MQ=255)                                                                                                                                                                                                      tttCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTg                                                                                                   >  1:11860/1‑196 (MQ=255)                                                                                                                                                                                                          tcCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCtt                                        <  1:330138/251‑1 (MQ=255)                                                                                                                                                                                                           ccATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCaaa                                                                                                                                                    <  1:442161/142‑1 (MQ=255)                                                                                                                                                                                                              tttCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCtt                                        <  1:164653/247‑1 (MQ=255)                                                                                                                                                                                                                tCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGa                                                                                                                                                 >  1:472655/1‑140 (MQ=255)                                                                                                                                                                                                                tCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAgg                                                                    <  1:806786/217‑1 (MQ=255)                                                                                                                                                                                                                tCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCgcgc                                   >  1:245855/1‑250 (MQ=255)                                                                                                                                                                                                                tCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCgcgc                                   >  1:331978/1‑250 (MQ=255)                                                                                                                                                                                                                tCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCgcgc                                   >  1:553259/1‑250 (MQ=255)                                                                                                                                                                                                                tCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGACCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCgcgc                                   >  1:825465/1‑250 (MQ=255)                                                                                                                                                                                                                     atCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCtt                                        <  1:669654/240‑1 (MQ=255)                                                                                                                                                                                                                     atCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAAt                             >  1:555983/1‑251 (MQ=255)                                                                                                                                                                                                                     atCCAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAAt                             >  1:541382/1‑251 (MQ=255)                                                                                                                                                                                                                        cAGAAAGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGaa                          >  1:32510/1‑251 (MQ=255)                                                                                                                                                                                                                           aaaGATGGACGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGTAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGAAt                         <  1:439896/249‑1 (MQ=255)                                                                                                                                                                                                                                   aCGCCAAATCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGAATCATGCTGGCg               >  1:15229/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTCGTCGGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTTCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTTCGCAGTTGGTGCAt                                                                                                         >  1:34886/1‑154 (MQ=255)                                                                                                                                                                                                                                           tcgtcgGCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCATTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGAATCATGCTGGCGGTCGgcgc       <  1:454559/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGAATCATGCTGGCGGTCGGCGCAGAGc  <  1:281868/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGAATCATGCTGGCGGTCGGCGCAGAGc  <  1:102459/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gCGATAACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTCTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGAATCATGCTGGCGGTCGGCGCAGAGc  <  1:10262/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GTATCCGATGAAACAGGTGGGCACCGATGAAGTAGGATTTGCCGTACATCAAATTGATTTATCGGGCAAATAATCCAAAAGCAAAAGGCATCCGTTGGATGCCTTTTTTGTCGCGAAAGTGCGGTCATTTTTATGAATGTTTTTTATTATTCCGGCGGGAACACAAACGGGTTCAAGCCGCTTTTCATAAAGCCTTTTTTCTCCATTTCCATATCCAGAAAGATGGACGCCAAATCGTCGGCGATGACTTCCATTTTCGGATCTTTCTCTTGATATAGAGCTTTCAAATAGCTATGGCAGTTCCCACAGCTCTCACTTCGGATCGCCGCCAGGTATTCGTCAAAAGACCAATAATCCAAGTCGCTGCTTTGTCCGCAGTTGGTGCATTGGTTGCGCACCATGTTCCATTCGCTTTCGCACAAGGAGCAGTGCAGGTAACGCAAGCCTTGCTTCGCGCCGAAATGAATCATGCTGGCGGTCGGCGCAGAGC  >  1/1237782‑1238271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑