Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,234,824	C→T	E283K (GAA→AAA)	unknown ←	Iron compound ABC uptake transporter substrate‑binding protein PiuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,234,824	0	C	T	100.0%	230.7 / NA	77	E283K (GAA→AAA)	unknown	Iron compound ABC uptake transporter substrate‑binding protein PiuA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/45);  total (32/45)

GCATTTGCTCCGGTTGGGTAAAAAATTGTGCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTCCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGTTTATGCTTGGATCTGCCATCGGC  >  1/1234585‑1235062                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               gCATTTGCTCCGGTTGGGTAAAAAATTGTGCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCg                                                                                                                                                                                                                                     >  1:811619/1‑251 (MQ=255)          ccGGTTGGGTAAAAAATTGTGCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGt                                                                                                                                                                                                                            <  1:868614/251‑1 (MQ=255)               tGGGTAAAAAATTGTGCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAagag                                                                                                                                                                                                                       <  1:91835/251‑1 (MQ=255)                  gTAAAAAATTGTGCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGgc                                                                                                                                                                                                                     <  1:895200/250‑1 (MQ=255)                             tgCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAAt                                                                                                                                                                                                          <  1:577286/250‑1 (MQ=255)                             tgCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATa                                                                                                                                                                                                         <  1:440665/251‑1 (MQ=255)                             tgCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATa                                                                                                                                                                                                         <  1:298556/251‑1 (MQ=255)                                   aTGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGat                                                                                                                                                                                                   <  1:35515/251‑1 (MQ=255)                                           ccGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGt                                                                                                                                                                                           >  1:464684/1‑251 (MQ=255)                                                 cacCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTc                                                                                                                                                                                      >  1:382576/1‑250 (MQ=255)                                                   ccGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAAt                                                                                                                                                                                                          <  1:595535/228‑1 (MQ=255)                                                      aCGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGc                                                                                                                                                                                 <  1:227858/250‑1 (MQ=255)                                                         gTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTg                                                                                                                                                                             >  1:562296/1‑251 (MQ=255)                                                            aTGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGgtg                                                                                                                                                                          >  1:168034/1‑251 (MQ=255)                                                              gctgcCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGGTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAgg                                                                                                                                                                                                                      <  1:208647/205‑1 (MQ=255)                                                                tgcCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGa                                                                                                                                                                       >  1:72747/1‑250 (MQ=255)                                                                 gcCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGa                                                                                                                                                                       <  1:349993/249‑1 (MQ=255)                                                                          gAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAGGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGaattaatt                                                                                                                                                             <  1:457836/250‑1 (MQ=255)                                                                          gAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGc                                                                                                                                                                                 <  1:236196/230‑1 (MQ=255)                                                                          gAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGaattaatt                                                                                                                                                             >  1:473275/1‑250 (MQ=255)                                                                            gTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTaaa                                                                                                                                                                                                           >  1:100570/1‑202 (MQ=255)                                                                                aaGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTAt                                                                                                                                                       >  1:787319/1‑250 (MQ=255)                                                                                      aaGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATAc                                                                                                                                                <  1:175425/251‑1 (MQ=255)                                                                                         gACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATCTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATActt                                                                                                                                              >  1:743825/1‑250 (MQ=255)                                                                                                        gTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATacac                                                                                                                                                                                                      >  1:752105/1‑179 (MQ=255)                                                                                                             gggTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGaatta                                                                                                                                                                <  1:394657/212‑1 (MQ=255)                                                                                                             gggTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTc                                                                                                                           >  1:125331/1‑249 (MQ=255)                                                                                                              ggTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTc                                                                                                                           <  1:692719/248‑1 (MQ=255)                                                                                                              ggTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTc                                                                                                                           <  1:71438/248‑1 (MQ=255)                                                                                                                    aaTGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGAt                                                                                                                  <  1:342806/251‑1 (MQ=255)                                                                                                                       gTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATac                                                                                                                                                                                                        <  1:3648/162‑1 (MQ=255)                                                                                                                         tCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCgg                                                                                                             <  1:92208/251‑1 (MQ=255)                                                                                                                            gCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGaa                                                                                                           <  1:538711/250‑1 (MQ=255)                                                                                                                              aTGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATg                                                                                                        <  1:247661/251‑1 (MQ=255)                                                                                                                                ggTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGgcgc                                                                                                                                                                                                                                 <  1:389975/128‑1 (MQ=255)                                                                                                                                ggTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCg                                                                                                                                                                                            >  1:146199/1‑165 (MQ=255)                                                                                                                                ggTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTc                                                                                                                           >  1:312652/1‑230 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAagag                                                                                                                                                                                                                       >  1:293910/1‑137 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATa                                                                                                                                                                                                         <  1:109845/151‑1 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTgg                                                                                                                                        <  1:418402/216‑1 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTcc                                                                                                                          <  1:345499/230‑1 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATg                                                                                                        >  1:403345/1‑248 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGa                                                                                                     >  1:322797/1‑251 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGa                                                                                                     >  1:817122/1‑251 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGa                                                                                                     >  1:174022/1‑251 (MQ=255)                                                                                                                                 gTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTCGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGa                                                                                                     >  1:528420/1‑251 (MQ=255)                                                                                                                                       aCATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAag                                                                                                                                                                                                                         >  1:485537/1‑129 (MQ=255)                                                                                                                                        cATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAaca                                                                                              >  1:210239/1‑251 (MQ=255)                                                                                                                                             aaaaGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACacaaac                                                                                         <  1:113858/251‑1 (MQ=255)                                                                                                                                                 ggTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAaacaac                                                                                      >  1:108367/1‑250 (MQ=255)                                                                                                                                                  gTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCa                                                                                    >  1:575975/1‑251 (MQ=255)                                                                                                                                                     ctctcCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATc                                                                                 <  1:280112/251‑1 (MQ=255)                                                                                                                                                     ctctcCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATc                                                                                 <  1:665937/251‑1 (MQ=255)                                                                                                                                                       ctcCCGCAGGAGAAAGCCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAgg                                                                                                                                                                                  <  1:168156/152‑1 (MQ=255)                                                                                                                                                       ctcCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCg                                                                               >  1:739182/1‑251 (MQ=255)                                                                                                                                                           cGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGAtt                                                                           <  1:699845/251‑1 (MQ=255)                                                                                                                                                             cAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATa                                                                                                                                                                                                         <  1:794064/123‑1 (MQ=255)                                                                                                                                                                       ccGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATc                                                                                 <  1:200817/233‑1 (MQ=255)                                                                                                                                                                                gATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCaaa                                                      >  1:53502/1‑251 (MQ=255)                                                                                                                                                                                 aTTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGa                                                                                                                                                                                                    <  1:244206/108‑1 (MQ=255)                                                                                                                                                                                  ttCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAAc                                                    <  1:10184/251‑1 (MQ=255)                                                                                                                                                                                       cggATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATc                                               <  1:502772/251‑1 (MQ=255)                                                                                                                                                                                          attattTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTATGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGt                                            <  1:75306/251‑1 (MQ=255)                                                                                                                                                                                          attattTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGt                                            <  1:323535/251‑1 (MQ=255)                                                                                                                                                                                             attTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGa                                         >  1:521611/1‑251 (MQ=255)                                                                                                                                                                                                    ctcAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGAtt                                                                           >  1:704623/1‑210 (MQ=255)                                                                                                                                                                                                    ctcAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGAc                                  <  1:173316/251‑1 (MQ=255)                                                                                                                                                                                                    ctcAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGAc                                  <  1:589556/251‑1 (MQ=255)                                                                                                                                                                                                              gcTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCa                                      <  1:849629/237‑1 (MQ=255)                                                                                                                                                                                                              gcTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGtt                        >  1:752289/1‑251 (MQ=255)                                                                                                                                                                                                              gcTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGt                         <  1:420174/250‑1 (MQ=255)                                                                                                                                                                                                              gcTTTGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCACAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGtt                        >  1:684396/1‑251 (MQ=255)                                                                                                                                                                                                                  tGATGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGTTTATg                    <  1:547682/251‑1 (MQ=255)                                                                                                                                                                                                                    aTGTTATTCAAATCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGTTTATGc                   <  1:36462/250‑1 (MQ=255)                                                                                                                                                                                                                              aaTCCATTTTTAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGTTTATGCTTGGATCTGc         >  1:328166/1‑250 (MQ=255)                                                                                                                                                                                                                                    tttttAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGTTTATGCTTGGATCTGCCa       >  1:67128/1‑246 (MQ=255)                                                                                                                                                                                                                                    tttttAATTGTTTCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGTTTATGCTTGGATCTGCCATCGGc  <  1:584783/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               GCATTTGCTCCGGTTGGGTAAAAAATTGTGCCCAATGAAAATCCGCAACACCGACGGTAATGCTGCCCACGGTGAGTAAAAGGAGAAGGACAAAATTAATTCGGTTCAGGGTGAAAATGTTCGGCATGGTATTAACATAAAAAAGGTTCTCTCCCGCAGGAGAAAACCGTTAAAAGATTCGGCGGATTATTTTTTCTCAAAGGCGGCTTTGATGTTATTCAAATCCATTTTTAATTGTTCCGCACCACCCGGCGCAAGGTAAGAGGCGCTGCTTAAATACACGATATTGCCGTTTTTCCAGGCTTTGGTGTGACGAATTAATTCGTTATCCAATACTTCTTTGGCGCCTTTCCCTTCCTGCCCGATAGCGGAAATGCGATCTAACACAAACAACCAATCCGGATTGGTTTTCTCAATAAATTCAAAACTGATCGGTTGACCATGACCGCTGACGTTTATGCTTGGATCTGCCATCGGC  >  1/1234585‑1235062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑