| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,231,865 | C→T | S487S (AGC→AGT) | unknown → | Murein DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,231,865 | 0 | C | T | 98.8% | 216.6 / NA | 83 | S487S (AGC→AGT) | unknown | Murein DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (32/48); total (35/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCCTATCAGGCTTACGGTGCCGGTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGCAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATATGATGCACCACTAAACGCTTTTCTTGATGTTCCAGCACTTCCGCGTCCAGATTAAATA > 1/1231627‑1232109 | tCGCCTATCAGGCTTACGGTGCCGGTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGt < 1:503491/251‑1 (MQ=255) cGCCTATCAGGCTTACGGTGCCGGTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTa < 1:318406/251‑1 (MQ=255) cGCCTATCAGGCTTACGGTGCCGGTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTa < 1:863821/251‑1 (MQ=255) ccTATCAGGCTTACGGTGCCGGTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTAc < 1:654052/250‑1 (MQ=255) aTCAGGCTTACGGTGCCGGTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTAcggcg < 1:112642/251‑1 (MQ=255) gTGCCGGTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACa < 1:336621/250‑1 (MQ=255) ggTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTa < 1:222131/229‑1 (MQ=255) gTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGGACGGCGGAACGTAAACAATTCCtt > 1:152731/1‑251 (MQ=255) gTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGtt > 1:318095/1‑251 (MQ=255) gTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGGACGGCGGAACGTAAACAATTCCTTGtt > 1:690992/1‑251 (MQ=255) gTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCCCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGCACGTAAACAATTCCTTGtt > 1:5000/1‑251 (MQ=255) gTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTAACGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTAACGTTAAACTTCCGGGGGGAAGTAGCGGGATAGGTACGGCGGAACGTAAACAATTTCTTGtt > 1:212342/1‑251 (MQ=255) aTGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAAATTCCGGGGGGAAGTAGCGGCATAGGTAcggcgg > 1:812434/1‑228 (MQ=255) gtgCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGGAAACAATTCCTTg > 1:476000/1‑241 (MQ=255) gCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGGCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGcc > 1:604515/1‑247 (MQ=255) cccGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGc < 1:756979/251‑1 (MQ=255) cGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCCGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCCGTCCATCCGCTTACCGTTAAACTTCCGGGGGGAAGTTGCGGCATAGGGACGGCGGAACGGAAACCATTCCTTGtttctgtctcttattcacaaccccg > 1:37477/1‑228 (MQ=255) gAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCCTTAAACTTCCGGGGGGAAGTAGCGGCATAGGGACGGCGGAACGTAAAAAATTCCTTGTTAAAGCCAACGAAGGAGAACGCttgtt > 1:333798/1‑251 (MQ=255) aaTACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCttgttg < 1:460878/251‑1 (MQ=255) aTACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAg < 1:519181/239‑1 (MQ=255) aCCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTgcg > 1:418537/1‑250 (MQ=255) aCCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGt < 1:899370/215‑1 (MQ=255) aCCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGGACGGCGGAACGTAAAAAAATCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCCGAtt > 1:42227/1‑251 (MQ=255) ccGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCCGCCCGCATTTGCACCACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGGACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGAttt > 1:502759/1‑251 (MQ=255) cGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTc < 1:803690/251‑1 (MQ=255) gtgtATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGGTTTCa > 1:501763/1‑251 (MQ=255) gtgtATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGGCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGGACGGCGGAACGTAAACAATTCCTTGTTAAAGcc > 1:206410/1‑219 (MQ=255) gtgtATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGGCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGGACGGCGGAACGTAAAAAATTCCTTGTTAAAACCAACGAAGTAGAACGCTTGTTGCGCTGAtttta > 1:419316/1‑249 (MQ=255) tgtATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGa < 1:377596/223‑1 (MQ=255) atatGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAAc > 1:286934/1‑250 (MQ=255) cACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGcc < 1:344507/210‑1 (MQ=255) cACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAAAGGGCGTCCGGGCAATCCGCCTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGGAAACAATTc > 1:723037/1‑198 (MQ=255) cACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCCCATTTGCACTACGAAATTCATATTAACGGGCGGCCGGTCAATCCGCTTACCGTTAAACTTCCCGGGGGAAGAAGCGGCATAGGTACGGCGGAACGGAAACAATTCCTTTTTAAAGCCAACGAAGTAGAAc > 1:255465/1‑223 (MQ=255) ccTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGGAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAAc > 1:111478/1‑243 (MQ=255) ccTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACCTCCGGGGGGAAGTAGCGGCATAGGCACGGCGGAAAGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGttt > 1:378717/1‑249 (MQ=255) gcagACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACCTCCGGGGGGGAGGAGCGGCATAGGGACGGCGGAAAGTAAACAATTCCTTGTTa > 1:856928/1‑198 (MQ=255) gcagACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAAACCGCTTACCGTTAAACCTCCGGGGGGAAGCAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTTAAGCCAACGAAGTAGAACGCCTGCTGCGGTGGTTTCAAACCCCTCTCTaaaaa > 1:680256/1‑251 (MQ=255) gACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTc < 1:853141/188‑1 (MQ=255) aGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCCAACCCGTTTCTTAAAAGTGCGGTggg > 1:785688/1‑250 (MQ=255) aGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGGACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAggg > 1:209401/1‑242 (MQ=255) aaGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGACAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGAttttt > 1:471097/1‑251 (MQ=255) ggCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGGTTTCAAAACCGTTTCTAAAAAGTGCGGGGGATTTTTTAAgtg > 1:674674/1‑251 (MQ=255) ggCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAgtg > 1:199475/1‑251 (MQ=255) ggCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAg < 1:763026/249‑1 (MQ=255) ggCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAg < 1:573890/249‑1 (MQ=255) ggCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAg < 1:750842/249‑1 (MQ=255) gCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAgtg < 1:561449/250‑1 (MQ=255) gTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTgcgc < 1:91358/198‑1 (MQ=255) aaaaaGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAg < 1:619812/237‑1 (MQ=255) aaaaaGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGGGGATTTTTTAAGTGGTTTTAAAAaca > 1:17955/1‑251 (MQ=255) aaaGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGc < 1:126590/183‑1 (MQ=255) gCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTAcggcgg < 1:638865/134‑1 (MQ=255) tATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAGTTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAacc < 1:236956/250‑1 (MQ=255) gcgTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTc > 1:17705/1‑141 (MQ=255) gTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTAc > 1:727730/1‑117 (MQ=255) cAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGTGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTg < 1:704989/251‑1 (MQ=255) aaCACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGt < 1:275546/251‑1 (MQ=255) cggTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTaataaat < 1:257414/250‑1 (MQ=255) gTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTaataaat > 1:718259/1‑248 (MQ=255) tCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATaaaga < 1:478998/251‑1 (MQ=255) ccGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGt < 1:560074/251‑1 (MQ=255) cccGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGc < 1:118984/250‑1 (MQ=255) cccGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGc < 1:505457/250‑1 (MQ=255) cATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAAt < 1:759211/250‑1 (MQ=255) gCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGtaa < 1:590740/224‑1 (MQ=255) aCTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACata < 1:780167/251‑1 (MQ=255) cTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCg < 1:477872/206‑1 (MQ=255) aCGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAAcc < 1:42206/143‑1 (MQ=255) cGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATatgat < 1:283357/251‑1 (MQ=255) aaTTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAAcc < 1:204459/140‑1 (MQ=255) ttCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATatga < 1:584733/246‑1 (MQ=255) atatTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCAc > 1:650608/1‑198 (MQ=255) tAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATATGATGCACCACTAAAc < 1:174872/251‑1 (MQ=255) aaCGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTgaga > 1:657978/1‑181 (MQ=255) cGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATATGATGCACCACTAAACGCTTTTc < 1:561541/251‑1 (MQ=255) ccGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATATGATGCACCACTAAACGCTTTTCTTg < 1:167696/251‑1 (MQ=255) gTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTgcgc < 1:682287/105‑1 (MQ=255) ccGCTTACCGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATATGATGc < 1:405557/222‑1 (MQ=255) ccGTTAAACTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACat < 1:727704/208‑1 (MQ=255) cTTCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATATGATGCACCACTAAACGCTTTTCTTGATGTTCCAGCACTTCCGCGTCCAg > 1:282084/1‑251 (MQ=255) tCCGGGGGGAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACat < 1:362830/198‑1 (MQ=255) gggAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTgaga < 1:410688/140‑1 (MQ=255) ggAAGTAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATATGATGCACCACTAAACGCTTTTCTTGATGTTCCAGCACTTCCGCGTCCAGATTAAata < 1:558014/250‑1 (MQ=255) | TCGCCTATCAGGCTTACGGTGCCGGTCGTTATGTGGTGCTACGCCACTCCCGTGAATACCAAACCGTGTATATGCACCTCAGCAGACCGCTAGTAAAAGTAGGGCAAACAGTGAAAAAAGGGGAGCGTATCGCGTTGTCCGGCAACACCGGCGGTTCTACCGGCCCGCATTTGCACTACGAATTCCATATTAACGGGCGTCCGGTCAATCCGCTTACCGTTAAACTTCCGGGGGGAAGCAGCGGCATAGGTACGGCGGAACGTAAACAATTCCTTGTTAAAGCCAACGAAGTAGAACGCTTGTTGCGCTGATTTCAAACCCGTTTCTAAAAAGTGCGGTGGATTTTTTAAGTGTTTTTAAAAACACCTGAGAAAACCACCGCACTTTTCTTTGTAATAAATAAAGAAAGTAGCAAATACTACATATGATGCACCACTAAACGCTTTTCTTGATGTTCCAGCACTTCCGCGTCCAGATTAAATA > 1/1231627‑1232109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |