Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,218,808 C→T intergenic (‑175/‑81) unknown ← / → unknown Malate dehydrogenase (EC 1.1.1.37)/Arginine pathway regulatory protein ArgR, repressor of arg regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,218,8080CT100.0% 62.4 / NA 23intergenic (‑175/‑81)unknown/unknownMalate dehydrogenase (EC 1.1.1.37)/Arginine pathway regulatory protein ArgR, repressor of arg regulon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/15);  total (8/15)

TTGTAACTTCAGTAATAATGCCAATGCTTGACCGATACCGCCTGCGGCACCTAAAACAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCCGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTGAATAAAAATCCGGACAATCTGGTCAAAGCATTTAAAACTTTATTACGCGAAGAAAAATACGGTTCACAAACGGAAATTGCCGCCGAGCTTCAAAAACTTGGTTTTCGTAGCGTAAACC  >  1/1218565‑1219012
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                            
ttGTAACTTCAGTAATAATGCCAATGCTTGACCGATACCGCCTGCGGCACCTAAAACAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGttt                                                                                                                                                                                                          <  1:172700/248‑1 (MQ=255)
                        tGCTTGACCGATACCGCCTGCGGCACCTAAAACAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCa                                                                                                                                                                               >  1:268940/1‑251 (MQ=255)
                                  aTACCGCCTGCGGCACCTAAAACAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGttt                                                                                                                                                                                                          >  1:659306/1‑214 (MQ=255)
                                  aTACCGCCTGCGGCACCTAAAACAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTAt                                                                                                                                                                     >  1:774904/1‑251 (MQ=255)
                                                aCCTAAAACAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGc                                                                                                                                                                           <  1:897674/231‑1 (MQ=255)
                                                 ccTAAAACAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCAc                                                                                                                                                      <  1:249521/251‑1 (MQ=255)
                                                     aaaCAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTAt                                                                                                                                                  >  1:288355/1‑251 (MQ=255)
                                                        cAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGtt                                                                                                                                               >  1:742296/1‑251 (MQ=255)
                                                                        attCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAAcc                                                                                                                               <  1:562895/251‑1 (MQ=255)
                                                                             cctcATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTGTTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGt                                                                                                                          <  1:164674/251‑1 (MQ=255)
                                                                               tcATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTg                                                                                                                        <  1:152301/251‑1 (MQ=255)
                                                                                      gtaAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGttt                                                                                                                                                                                                          <  1:893633/162‑1 (MQ=255)
                                                                                      gtaAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTGAATaaaa                                                                                                                 >  1:163267/1‑251 (MQ=255)
                                                                                       taAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTGAATaaaaa                                                                                                                <  1:730544/251‑1 (MQ=255)
                                                                                                    gtTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTa                                                                                                                                                                                     <  1:850940/169‑1 (MQ=255)
                                                                                                                     aaTTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTGAATAAAAATCCGGACAATCTGGTCAAAGCATTTAAAAc                                                                                  <  1:649676/251‑1 (MQ=255)
                                                                                                                         gTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGAc                                                                                                                                    <  1:471178/197‑1 (MQ=255)
                                                                                                                                            aaTTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTGAATAAAAATCCGGACAATCTGGTCAAAGCATTTAAAACTTTATTATGCGAAGAAAAATACg                                                           >  1:257806/1‑251 (MQ=255)
                                                                                                                                                           gTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAAtatttattc                                                                                                                                                                                         <  1:797215/110‑2 (MQ=255)
                                                                                                                                                                                            tttCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTGAATAAAAATCCGGACAATCTGGTCAAAGCATTTAAAACTTTATTATGCGAAGAAAAATACGGTTCACAAACGGAAATTGCCGCCGAGCTTCAAAAACTTGGTTTTCGTa           >  1:865667/1‑251 (MQ=255)
                                                                                                                                                                                                     aaCATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTGAATAAAAATCCGGACAATCTGGTCAAAGCATTTAAAACTTTATTATGCGAAGAAAAATACGGTTCACAAACGGAAATTGCCGCCGAGCTTCAAAAACTTGGTTTTCGTAGCGTAAAcc  <  1:53703/251‑1 (MQ=255)
                                                                                                                                                                                                                  caAAAAACATAATTTTTTATGACCGCACTTTTCTGTTttaatt                                                                                                                                                                                                     <  1:676789/43‑1 (MQ=255)
                                                                                                                                                                                                                                               tttCTGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGt                                                                                                                                                <  1:766560/67‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                            
TTGTAACTTCAGTAATAATGCCAATGCTTGACCGATACCGCCTGCGGCACCTAAAACAGCAACTTTCATTTCATTCTCCTCATGTAGTAAAAAATAGGTGGTTAAAAAATCTGAAAAAATTGTACGCTGATCAATTTTTAAATTCAAACAGGCAGGTGCAAATGTGCGATCTAACTCAAATTTTTTATTTTCTATTGAACATAAAAATCACAAAAAACATAATTTTTTATGACCGCACTTTTCCGTTTTAATTAAATATTTATTTGCTAAAGTCACTGCTTTTATGCAAAATTCAGCCACTTATGTTCCACAAGAGACAAACCTATGTTGAATAAAAATCCGGACAATCTGGTCAAAGCATTTAAAACTTTATTACGCGAAGAAAAATACGGTTCACAAACGGAAATTGCCGCCGAGCTTCAAAAACTTGGTTTTCGTAGCGTAAACC  >  1/1218565‑1219012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑