Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,217,214	T→C	I138T (ATT→ACT)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,217,214	0	T	C	100.0%	109.6 / NA	37	I138T (ATT→ACT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/21);  total (16/21)

TAATTTGGGTAAGTATTATCCTCTTGGCAACTTATTTAGTTGGCTTGTTTGGGTTAAAAAAAGCCAGACCATTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATATTCACAAGCACATCGGTACGATTTCTATCAACACGACTCTCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACGTATTAGCAAAATACAGTGACACTTTTATCTTAGCGAAAAGTACTCGTGCTACTAATGAC  >  1/1216974‑1217455                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  tAATTTGGGTAAGTATTATCCTCTTGGCAACTTATTTAGTTGGCTTGTTTGGGTTAAAAAAAGCCAGACCATTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGcac                                                                                                                                                                                                                                         <  1:2770/251‑1 (MQ=255)                  atCCTCTTGGCAACTTATTTAGTTGGCTTGTTTGGGTTAAAAAAAGCCAGACCATTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTAt                                                                                                                                                                                                                        >  1:52860/1‑251 (MQ=255)                   tCCTCTTGGCAACTTATTTAGTTGGCTTGTTTGGGTTAAAAAAAGCCAGACCATTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATc                                                                                                                                                                                                                       <  1:568391/251‑1 (MQ=255)                               cTTATTTAGTTGGCTTGTTTGGGTTAAAAAAAGCCAGACCATTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCaa                                                                                                                                                                                                                     >  1:510505/1‑241 (MQ=255)                                          ggCTTGTTTGGGTTAAAAAAAGCCAGACCATTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGActat                                                                                                                                                                                                            <  1:321986/239‑3 (MQ=255)                                                                       aTTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACAt                                                                                                                                                                                                                                       <  1:589725/183‑1 (MQ=255)                                                                       aTTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGAttt                                                                                                                                                                                                                            <  1:601038/194‑1 (MQ=255)                                                                                  aTTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTAt                                                                                                                                                        <  1:43700/251‑1 (MQ=255)                                                                                      cTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTAttt                                                                                                                                                      >  1:325748/1‑249 (MQ=255)                                                                                             aaTCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATcgcg                                                                                                                                                                                                        <  1:556309/192‑1 (MQ=255)                                                                                                          cTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATcg                                                                                                                                                                                                          >  1:34594/1‑177 (MQ=255)                                                                                                          cTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTAc                                                                                                                                                                  <  1:259593/217‑1 (MQ=255)                                                                                                          cTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTAtcgt                                                                                                                                 >  1:805659/1‑250 (MQ=255)                                                                                                          cTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTAtcgt                                                                                                                                 >  1:770713/1‑250 (MQ=255)                                                                                                                    gtgtAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTcc                                                                                                                       >  1:846402/1‑250 (MQ=255)                                                                                                                           gTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATc                                                                                                                                                                                                                       <  1:340229/147‑1 (MQ=255)                                                                                                                           gTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGActat                                                                                                                                                                                                            <  1:418161/158‑3 (MQ=255)                                                                                                                               tcttTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACAttt                                                                                                            >  1:457845/1‑250 (MQ=255)                                                                                                                                                   ggTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTg                                                                                                                                                    <  1:465678/190‑1 (MQ=255)                                                                                                                                                        cATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGtt                                                                                                                                       <  1:114795/198‑1 (MQ=255)                                                                                                                                                                  gTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCaa                                                                        >  1:76464/1‑251 (MQ=255)                                                                                                                                                                       aaaaGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGcc                                                                    <  1:109376/250‑1 (MQ=255)                                                                                                                                                                           gTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTcc                                                                                                                       >  1:848162/1‑195 (MQ=255)                                                                                                                                                                            ttAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTa                                                               <  1:422394/250‑1 (MQ=255)                                                                                                                                                                             tAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACg                                                             <  1:821688/251‑1 (MQ=255)                                                                                                                                                                                           cAATCTTCTATATTGGCATTACTGTTATCTTTACTCTTTTGTTGAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATGTATGTGATGTTGGCTGCCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGGCTCATTAGAAATTGAAGGCAAAGCCTATTACGTATTAGCAAAATAc                                               <  1:346634/251‑1 (MQ=255)                                                                                                                                                                                             aTCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTAtcg                                                                                                                                  >  1:49950/1‑166 (MQ=255)                                                                                                                                                                                               cttctATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCg                                                                                                                                         >  1:584168/1‑157 (MQ=255)                                                                                                                                                                                               cttctATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACGTATTAGCAAAATAc                                               <  1:208004/247‑1 (MQ=255)                                                                                                                                                                                                    atatTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTAtcgtc                                                                                                                                <  1:895919/161‑1 (MQ=255)                                                                                                                                                                                                    atatTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTAc                                                              >  1:867881/1‑227 (MQ=255)                                                                                                                                                                                                         ggCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACGt                                                            >  1:221855/1‑224 (MQ=255)                                                                                                                                                                                                               aCTTTTATCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAac                                                                                                                                                                                                                    <  1:668745/66‑1 (MQ=255)                                                                                                                                                                                                                      tCTTTACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACGTATTAGCAAAATACAGTGACACTTTTATCTTAGCGAAAAGt                    >  1:22747/1‑251 (MQ=255)                                                                                                                                                                                                                         ttACTCTTTTGTTTAAAAATTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACGTATTAGCAAAATACAGTGACACTTTTATc                                <  1:573650/236‑1 (MQ=255)                                                                                                                                                                                                                                        aaaaTTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACGTATTAGCAAAATACAGTGACACTTTTATCTTAGCGAAAAGTACTCGTGCTACTAATGAc  <  1:146566/251‑1 (MQ=255)                                                                                                                                                                                                                                           aTTATACTCACAAGCACATCGGTACGATTTCTATCAACACGACTATCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACGTATTAGCAAAATACAGTGacac                                       >  1:59425/1‑211 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  TAATTTGGGTAAGTATTATCCTCTTGGCAACTTATTTAGTTGGCTTGTTTGGGTTAAAAAAAGCCAGACCATTCATGACCGATTACTGCATGAATCTGTTGCGGACTTATATTCTGTGTAATGTCTTCTTTATTCCGGTTATTCTTGGTTACATCCTGACAGTTGGAAAAGTTAATTATCTTTTCGCAATCTTCTATATTGGCATTACTTTTATCTTTACTCTTTTGTTTAAAAATTATATTCACAAGCACATCGGTACGATTTCTATCAACACGACTCTCGCGTTTTTATATAAAAATAAAATTTATGTGATGTTGGCTACCTATTGTTATTTTGTAATATGTGCGTTTATCGTCGGATATTCCCGCCCACATTTCAAAACAGTATTTGACTCATTAGAAATTGAAGGCAAAGCCTATTACGTATTAGCAAAATACAGTGACACTTTTATCTTAGCGAAAAGTACTCGTGCTACTAATGAC  >  1/1216974‑1217455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑