Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,179,088	G→A	A247V (GCG→GTG)	unknown ←	Enoyl‑[acyl‑carrier‑protein] reductase [NADH] (EC 1.3.1.9)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,179,088	0	G	A	100.0%	198.7 / NA	70	A247V (GCG→GTG)	unknown	Enoyl‑[acyl‑carrier‑protein] reductase [NADH] (EC 1.3.1.9)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/38);  total (32/38)

TAAAAAACCGTAAGCTTTATCAGTGCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCGCGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATCCGCTGCCATTACGCGGGTTGCCGC  >  1/1178859‑1179326                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               tAAAAAACCGTAAGCTTTATCAGTGTTTTTTACCGTGCCTTCCACGCGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTAAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGa                                                                                                                                                                                                                           <  1:263064/251‑1 (MQ=255)              gCTTTATCAGTGCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTaa                                                                                                                                                                                                              >  1:236521/1‑251 (MQ=255)               cTTTATCAGTGCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTaaa                                                                                                                                                                                                             >  1:694800/1‑251 (MQ=255)                tttATCAGTGCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCT‑ATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAAcc                                                                                                                                                                                                           <  1:814240/251‑1 (MQ=255)                 ttATCAGTGCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAAcc                                                                                                                                                                                                           <  1:265403/251‑1 (MQ=255)                 ttATCAGTGCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAAcc                                                                                                                                                                                                           <  1:605365/251‑1 (MQ=255)                 ttATCAGTGCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACAAAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAAcc                                                                                                                                                                                                           <  1:296836/251‑1 (MQ=255)                         gCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAAcc                                                                                                                                                                                                           <  1:804913/243‑1 (MQ=255)                             tttACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGaa                                                                                                                                                                                               <  1:381622/251‑1 (MQ=255)                             tttACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGaa                                                                                                                                                                                               <  1:349413/251‑1 (MQ=255)                                        ttCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGaa                                                                                                                                                                                                       <  1:159359/232‑1 (MQ=255)                                        ttCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGt                                                                                                                                                                                    <  1:720014/251‑1 (MQ=255)                                        ttCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTAAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATg                                                                                                                                                                                             >  1:531216/1‑242 (MQ=255)                                          ccACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGAt                                                                                                                                                                                                                                      <  1:673300/199‑1 (MQ=255)                                               cGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCcac                                                                                                                                                                             <  1:57733/251‑1 (MQ=255)                                               cGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCca                                                                                                                                                                              >  1:693906/1‑250 (MQ=255)                                               cGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCca                                                                                                                                                                              >  1:825158/1‑250 (MQ=255)                                               cGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTAAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCca                                                                                                                                                                              >  1:689555/1‑250 (MQ=255)                                                 ttCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGaa                                                                                                                                                                                               <  1:513685/231‑1 (MQ=255)                                                   cTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGAt                                                                                                                                                                                                                                      >  1:627080/1‑190 (MQ=255)                                                     ttGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGaa                                                                                                                                                                                               >  1:187565/1‑227 (MQ=255)                                                        cTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCaa                                                                                                                                                                     <  1:802085/250‑1 (MQ=255)                                                                aaTTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACgg                                                                                                                                                            <  1:288584/251‑1 (MQ=255)                                                                   ttgttgTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTgc                                                                                                                                                         <  1:449094/251‑1 (MQ=255)                                                                             aaTTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGc                                                                                                                                               <  1:901705/251‑1 (MQ=255)                                                                              aTTGGGCGAGTAGGGGATTATCTTGAAACATGACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCt                                                                                                                                                                                                                >  1:275996/1‑185 (MQ=255)                                                                                      aGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGtttt                                                                                                                                      >  1:754273/1‑251 (MQ=255)                                                                                            ggATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGc                                                                                                                                                                                                                        >  1:326518/1‑163 (MQ=255)                                                                                            ggATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGaa                                                                                                                                                                                               >  1:219075/1‑188 (MQ=255)                                                                                            ggATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATgg                                                                                                                                                                  >  1:199990/1‑217 (MQ=255)                                                                                                 aTCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAg                                                                                                                           >  1:370895/1‑251 (MQ=255)                                                                                                 aTCTTGAAACATAACGTCTAATAATTGGTAAAAAAACTAAAAAGGAAGGTAAAAAACCCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATg                                                                                                                                                                   <  1:376482/211‑1 (MQ=255)                                                                                                          cATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGt                                                                                                                                                    <  1:52213/217‑1 (MQ=255)                                                                                                               cGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTg                                                                                                                                                          <  1:56735/206‑1 (MQ=255)                                                                                                               cGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTg                                                                                                             >  1:110840/1‑251 (MQ=255)                                                                                                                gTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAgg                                                                                                                                                                                                 <  1:186143/166‑1 (MQ=255)                                                                                                                gTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATgg                                                                                                                                                                  <  1:560164/197‑1 (MQ=255)                                                                                                                gTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGa                                                                                                            >  1:542796/1‑251 (MQ=255)                                                                                                                  cTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAg                                                                                                          >  1:657994/1‑251 (MQ=255)                                                                                                                  cTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCAGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGt                                                                                                                                                    >  1:436030/1‑209 (MQ=255)                                                                                                                            gTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTgatg                                                                                                 >  1:168518/1‑250 (MQ=255)                                                                                                                                 aaaaCTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTgatgccggat                                                                                           <  1:544957/251‑1 (MQ=255)                                                                                                                                     cTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAATCGGGATCCACGTGCACGATTTCACCgg                                                                                                                                                                                                                              >  1:719247/1‑116 (MQ=255)                                                                                                                                     cTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCaa                                                                                                                                                                     <  1:864145/173‑1 (MQ=255)                                                                                                                                     cTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTgatgccggatgccg                                                                                       >  1:384198/1‑251 (MQ=255)                                                                                                                                     cTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTgatgccggatgcc                                                                                        >  1:257542/1‑250 (MQ=255)                                                                                                                                     cTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGCGCGGCGTAATGCCg                                                                                                                                             >  1:705978/1‑197 (MQ=255)                                                                                                                                         aaaGGCAGTTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCaa                                                                                   <  1:550559/251‑1 (MQ=255)                                                                                                                                            ggCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATgg                                                                                                                                                                  <  1:684930/169‑1 (MQ=255)                                                                                                                                                 gTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCCATGGTTACGGTGCGGCGTAATGCCGCAGGTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTgatgccggatgc                                                                                         >  1:750259/1‑237 (MQ=255)                                                                                                                                                 gTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAgg                                                                                 <  1:830191/245‑1 (MQ=255)                                                                                                                                                 gTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTAc                                                                              <  1:217241/248‑1 (MQ=255)                                                                                                                                                  ttAAAAACCCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAAt                                                                          <  1:341547/251‑1 (MQ=255)                                                                                                                                                  ttAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAAt                                                                          <  1:18429/251‑1 (MQ=255)                                                                                                                                                                 aTAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAAt                                                           <  1:612992/251‑1 (MQ=255)                                                                                                                                                                  tAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAAt                                                                          <  1:139157/235‑1 (MQ=255)                                                                                                                                                                   aaGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATgg                                                         <  1:711129/251‑1 (MQ=255)                                                                                                                                                                      tGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCg                                                       <  1:557706/250‑1 (MQ=255)                                                                                                                                                                            aagaaAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTcaca                                                 >  1:664287/1‑250 (MQ=255)                                                                                                                                                                             agaaAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGaa                                                                                                                                                                                               <  1:354491/107‑1 (MQ=255)                                                                                                                                                                                       attCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGt                                       >  1:136479/1‑249 (MQ=255)                                                                                                                                                                                               ccTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGGGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTTTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTaa                             >  1:424624/1‑251 (MQ=255)                                                                                                                                                                                                  aaTTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATc                          <  1:42844/251‑1 (MQ=255)                                                                                                                                                                                                  aaTTCTCCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGGACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAAt                           >  1:320298/1‑250 (MQ=255)                                                                                                                                                                                                      ctcCCATGGCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCGGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATCCg                        >  1:45318/1‑249 (MQ=255)                                                                                                                                                                                                             ggCGGTGATGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATCCGCTGCCATTa               >  1:502533/1‑251 (MQ=255)                                                                                                                                                                                                                    aTGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATCCGCTGCCATTACGCggg         <  1:188895/250‑1 (MQ=255)                                                                                                                                                                                                                    aTGCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATCCGCTGCCATTACGCGGGt        >  1:733112/1‑251 (MQ=255)                                                                                                                                                                                                                      gCTGAAACCGGCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATCCGCTGCCATTACGCGGGTTg      >  1:190420/1‑251 (MQ=255)                                                                                                                                                                                                                               ggCATCCACGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATCCGCTGCCATTACGCGGGTTGCCGc  <  1:206575/246‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               TAAAAAACCGTAAGCTTTATCAGTGCTTTTTACCGTGCCTTCCACACGTTCTTTGCTGTCGTGAATTTGTTGTTTTAATTGGGCGAGTAGGGGATTATCTTGAAACATAACGTCTAATAATTGGTATAAAAACTAAAAAGGCAGGTTAAAAAACCTGCCGATAAGTGAAGGAAGAAAAAATTATTCTTCGCCTAATTCTCCCATGGCGGTGATGCTGAAACCGGCATCCGCGTGCACGATTTCACCGGTGATGCCGGAAGCTAAACCAGAACATAGGAATGCGGCGGAGTTGCCCACATCTTCAATGGTTACGGTGCGGCGTAATGCCGCAGTTTTCTCAAAAGCAGAAAGCATTTTCTTGAAGTTTTTGATGCCGGATGCCGCCAAGGTACGAATCGGGCCGGCAGAAATGGCGTTCACACGAATGCCGTCTTTGCCTAAATCCGCTGCCATTACGCGGGTTGCCGC  >  1/1178859‑1179326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑