Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,165,328	A→G	L33P (CTG→CCG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,165,328	0	A	G	100.0%	183.1 / NA	64	L33P (CTG→CCG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/30);  total (34/30)

GCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACAGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTATTTTAAGCTGCCCACCATATCTTCCGGACGCACCCATTTGT  >  1/1165091‑1165572                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     gCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTtat                                                                                                                                                                                                                                          <  1:484251/250‑1 (MQ=255)    aCAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATc                                                                                                                                                                                                                                      <  1:864636/251‑1 (MQ=255)    aCAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATc                                                                                                                                                                                                                                      <  1:838029/251‑1 (MQ=255)    aCAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATc                                                                                                                                                                                                                                      <  1:32751/251‑1 (MQ=255)                             agaAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTg                                                                                                                                                                                                              <  1:100272/250‑1 (MQ=255)                                 gTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATc                                                                                                                                                                                                                                      >  1:316654/1‑222 (MQ=255)                                     ggAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAg                                                                                                                                                                                                     >  1:599442/1‑251 (MQ=255)                                                ctgcATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGaa                                                                                                                                                                                          <  1:305070/251‑1 (MQ=255)                                                  gcATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATc                                                                                                                                                                                                                                      >  1:520967/1‑205 (MQ=255)                                                                  caAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAAt                                                                                                                                                                                        <  1:814700/235‑1 (MQ=255)                                                                  caAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATg                                                                                                                                                                            <  1:79752/247‑1 (MQ=255)                                                                  caAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATa                                                                                                                                                                        >  1:898911/1‑251 (MQ=255)                                                                    aaTCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAAc                                                                                                                                                                      <  1:326707/251‑1 (MQ=255)                                                                      tCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAg                                                                                                                                                                    <  1:238476/251‑1 (MQ=255)                                                                        gCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCg                                                                                                                                                                  >  1:358232/1‑251 (MQ=255)                                                                            tGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGAttt                                                                                                                                                              <  1:311778/251‑1 (MQ=255)                                                                             gcgcTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGt                                                                                                                                                                                               >  1:5383/1‑217 (MQ=255)                                                                             gcgcTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGt                                                                                                                                                                                   <  1:551045/229‑1 (MQ=255)                                                                             gcgcTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGAtttt                                                                                                                                                             >  1:821568/1‑251 (MQ=255)                                                                                         cGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATaaaa                                                                                                                                                 >  1:511722/1‑251 (MQ=255)                                                                                         cGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATaaaa                                                                                                                                                 >  1:395762/1‑251 (MQ=255)                                                                                         cGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATaaaa                                                                                                                                                 >  1:754523/1‑251 (MQ=255)                                                                                                  gTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACGTTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCa                                                                                                                                        >  1:452885/1‑251 (MQ=255)                                                                                                  gTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAg                                                                                                                                               <  1:384175/244‑1 (MQ=255)                                                                                                   ttCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCAt                                                                                                                                       >  1:768485/1‑251 (MQ=255)                                                                                                   ttCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCAt                                                                                                                                       >  1:873348/1‑251 (MQ=255)                                                                                                          tctttcGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAAt                                                                                                                                                                                        >  1:848317/1‑195 (MQ=255)                                                                                                                    ggCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCg                                                                                                                      >  1:578345/1‑251 (MQ=255)                                                                                                                                          ttCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTCAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGaa                                                                                                >  1:431150/1‑251 (MQ=255)                                                                                                                                          ttCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGaa                                                                                                >  1:883099/1‑251 (MQ=255)                                                                                                                                           tCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATtat                                                                                                                 <  1:229193/233‑1 (MQ=255)                                                                                                                                            cAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAAt                                                                                              >  1:150633/1‑251 (MQ=255)                                                                                                                                                   tttttCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATccc                                                                                       <  1:843699/251‑1 (MQ=255)                                                                                                                                                      ttCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGc                                                                                     >  1:72319/1‑250 (MQ=255)                                                                                                                                                          tATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTAtg                                                                                                                            >  1:102476/1‑207 (MQ=255)                                                                                                                                                          tATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCttt                                                                                 >  1:393663/1‑250 (MQ=255)                                                                                                                                                           aTTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGGTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGAt                                                                                          >  1:340005/1‑240 (MQ=255)                                                                                                                                                           aTTTAAAGGAAAGCATAAACGGCAACTAATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTttct                                                                               <  1:823903/251‑1 (MQ=255)                                                                                                                                                                  ggAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCattat                                                                        >  1:165736/1‑251 (MQ=255)                                                                                                                                                                       gCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAAt                                                                                                                                                                             >  1:640075/1‑145 (MQ=255)                                                                                                                                                                       gCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATg                                                                   >  1:653174/1‑251 (MQ=255)                                                                                                                                                                        cATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAAt                                                                    <  1:300729/249‑1 (MQ=255)                                                                                                                                                                              cGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGtttaat                                                            >  1:777241/1‑251 (MQ=255)                                                                                                                                                                                    cTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAg                                                                                                                                                                                                     <  1:538396/108‑1 (MQ=255)                                                                                                                                                                                    cTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAAtg                                                      <  1:338560/251‑1 (MQ=255)                                                                                                                                                                                    cTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAAtg                                                      <  1:571105/251‑1 (MQ=255)                                                                                                                                                                                      gATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAAtgtg                                                    >  1:419314/1‑251 (MQ=255)                                                                                                                                                                                            cTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGcc                                              <  1:848603/251‑1 (MQ=255)                                                                                                                                                                                               cATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTa                                           >  1:408762/1‑251 (MQ=255)                                                                                                                                                                                                    gCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAAtg                                                      <  1:208865/235‑1 (MQ=255)                                                                                                                                                                                                       ccGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCg                                                                                                                                                                                                                            >  1:481683/1‑66 (MQ=255)                                                                                                                                                                                                       ccGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTATTTTAAGc                                   <  1:757615/251‑1 (MQ=255)                                                                                                                                                                                                                 tttCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAAtg                                                      <  1:901281/222‑1 (MQ=255)                                                                                                                                                                                                                  ttCTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGtttaattt                                                          <  1:516611/217‑1 (MQ=255)                                                                                                                                                                                                                    cTAACTCGATGGTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTATTTTAAGCTGCCCACCATATc                      <  1:409382/251‑1 (MQ=255)                                                                                                                                                                                                                               gTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATgg                                                                  >  1:37979/1‑196 (MQ=255)                                                                                                                                                                                                                               gTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATgg                                                                  <  1:474032/196‑1 (MQ=255)                                                                                                                                                                                                                               gTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTAttt                                        >  1:266581/1‑222 (MQ=255)                                                                                                                                                                                                                               gTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTATTTTAAGCTGCCCACCATATCTTCCGGACGCa           >  1:664592/1‑251 (MQ=255)                                                                                                                                                                                                                               gTATAAACGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTATTTTAAGCTGCCCACCATATCTTCCGGACGCa           >  1:539157/1‑251 (MQ=255)                                                                                                                                                                                                                                     aCGAGGAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTAttt                                        <  1:779360/216‑1 (MQ=255)                                                                                                                                                                                                                                          gAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTAtg                                                                                                                            >  1:570287/1‑127 (MQ=255)                                                                                                                                                                                                                                          gAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGtttaattt                                                          <  1:875168/193‑1 (MQ=255)                                                                                                                                                                                                                                          gAACGGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTATTTTAAGCTGCCCACCATATCTTCCGGACGCACCCATTTGt  <  1:174253/249‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     GCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTTCAATGGGTTTTTCGTATTTAAAGGAAAGCATAAACGGCAACTGATTGGCTTCATAAGCCCCGTTTTCCGTTTCTAACTCGATGGTATAAACGAGGAACAGACCCCAGTTATAATCGGCGTTTCCGACATTGAGCCAGTGCGCCGAAAGTGCGGTAGGAAATAAAGTTAAAATGAATAACAGCGATTTTAATTTCATAAAAAGTCCGTCATAAAAATTTATGTGGGCGATTATAGCAAAAAAGACGGGAAATTGATCCCGCCTTTCTTCATTATTAATGGTTTAATTTAATGTGTAAGCCTTATTTTAAGCTGCCCACCATATCTTCCGGACGCACCCATTTGT  >  1/1165091‑1165572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑