Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,164,985	A→G	S147S (AGT→AGC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,164,985	0	A	G	100.0%	222.8 / NA	74	S147S (AGT→AGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/43);  total (31/43)

CATATCGGCTTTTTGTGCGGCATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTACTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTT  >  1/1164743‑1165229                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     cATATCGGCTTTTTGTGCGGCATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCgg                                                                                                                                                                                                                                              <  1:828460/251‑1 (MQ=255)             ttGTGCGGCATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACTACCAAAt                                                                                                                                                                                                                                  <  1:885642/251‑1 (MQ=255)             ttGTGCGGCATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAAt                                                                                                                                                                                                                                  >  1:287306/1‑251 (MQ=255)                    gCATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAAt                                                                                                                                                                                                                            >  1:445793/1‑250 (MQ=255)                     cATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAGATGCTAATc                                                                                                                                                                                                                           <  1:289150/250‑1 (MQ=255)                     cATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATc                                                                                                                                                                                                                           <  1:828005/250‑1 (MQ=255)                     cATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATc                                                                                                                                                                                                                           <  1:266793/250‑1 (MQ=255)                      aTTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGc                                                                                                                                                                                                                                <  1:310854/244‑1 (MQ=255)                      aTTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAg                                                                                                                                                                                                                         <  1:368137/251‑1 (MQ=255)                         aagcaagcAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAgcgc                                                                                                                                                                                                                      <  1:856624/251‑1 (MQ=255)                            caagcaaTTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTtg                                                                                                                                                                                                                   <  1:403850/251‑1 (MQ=255)                                   tttttAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCt                                                                                                                                                                                                                               >  1:619151/1‑232 (MQ=255)                                                tttATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAAtt                                                                                                                                                                                               <  1:377268/251‑1 (MQ=255)                                                tttATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAAtt                                                                                                                                                                                               <  1:594450/251‑1 (MQ=255)                                                      cccGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGAt                                                                                                                                                                                         >  1:166017/1‑251 (MQ=255)                                                       ccGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATc                                                                                                                                                                                                                           <  1:892198/216‑1 (MQ=255)                                                       ccGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATc                                                                                                                                                                                                                           >  1:870805/1‑216 (MQ=255)                                                           aGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGCTTGCCTTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCaaa                                                                                                                                                                                    >  1:152586/1‑251 (MQ=255)                                                              gAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAAt                                                                                                                                                                                  <  1:236331/250‑1 (MQ=255)                                                              gAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAAt                                                                                                                                                                                  <  1:403166/250‑1 (MQ=255)                                                              gAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATg                                                                                                                                                                                 >  1:532609/1‑251 (MQ=255)                                                              gAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGAGCCAAAAt                                                                                                                                                                                  <  1:874937/250‑1 (MQ=255)                                                               aaaTATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAAt                                                                                                                                                                                                >  1:136747/1‑235 (MQ=255)                                                               aaaTATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATgg                                                                                                                                                                                <  1:576837/251‑1 (MQ=255)                                                               aaaTATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATgg                                                                                                                                                                                >  1:888487/1‑251 (MQ=255)                                                               aaaTATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATgg                                                                                                                                                                                <  1:364811/251‑1 (MQ=255)                                                                    tGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGTCATCCAAATGCTAATCAGCGCTCGTACAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCg                                                                                                                                                                           <  1:612302/251‑1 (MQ=255)                                                                          gCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGttt                                                                                                                                                                        <  1:450539/248‑1 (MQ=255)                                                                           cAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATg                                                                                                                                                                                                                                 <  1:251007/190‑1 (MQ=255)                                                                              ttAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTTCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATg                                                                                                                                                                  <  1:426489/250‑1 (MQ=255)                                                                                 aCCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAAt                                                                                                                                                              >  1:346436/1‑251 (MQ=255)                                                                                     aaTTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATgg                                                                                                                                                                                <  1:268261/229‑1 (MQ=255)                                                                                            gATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTc                                                                                                                                                    >  1:213501/1‑250 (MQ=255)                                                                                                        gAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACa                                                                                                                                       >  1:607081/1‑251 (MQ=255)                                                                                                        gAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGCGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACa                                                                                                                                       >  1:657175/1‑251 (MQ=255)                                                                                                                             gTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGt                                                                                                                  >  1:418975/1‑251 (MQ=255)                                                                                                                                       ggCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCgg                                                                                                        >  1:353267/1‑251 (MQ=255)                                                                                                                                                 tCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAgc                                                                                                >  1:49771/1‑249 (MQ=255)                                                                                                                                                         cGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTATAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCa                                                                                         <  1:86110/248‑1 (MQ=255)                                                                                                                                                            gTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTg                                                                                     >  1:821928/1‑249 (MQ=255)                                                                                                                                                            gTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGc                                                                                    <  1:295232/250‑1 (MQ=255)                                                                                                                                                             tCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCt                                                                                   <  1:4795/250‑1 (MQ=255)                                                                                                                                                                 cTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAAt                                                                                                                                                                                  >  1:279809/1‑151 (MQ=255)                                                                                                                                                                 cTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCaa                                                                               >  1:526635/1‑250 (MQ=255)                                                                                                                                                                        cgtgcgtTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACaaa                                                                        <  1:744779/250‑1 (MQ=255)                                                                                                                                                                        cgtgcgtTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAAt                                                                       <  1:377702/251‑1 (MQ=255)                                                                                                                                                                           gcgtTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCg                                                                     >  1:324230/1‑250 (MQ=255)                                                                                                                                                                            cgtTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATc                                                                                                                                                                                                                           <  1:474480/99‑1 (MQ=255)                                                                                                                                                                                      tctcCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTa                                                         >  1:100333/1‑251 (MQ=255)                                                                                                                                                                                        tcCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATc                                                       <  1:23954/251‑1 (MQ=255)                                                                                                                                                                                        tcCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATc                                                       <  1:65067/251‑1 (MQ=255)                                                                                                                                                                                        tcCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATc                                                       <  1:145880/251‑1 (MQ=255)                                                                                                                                                                                        tcCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGCTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATc                                                       <  1:677887/251‑1 (MQ=255)                                                                                                                                                                                         ccGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATcc                                                      <  1:343927/251‑1 (MQ=255)                                                                                                                                                                                         ccGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATcc                                                      <  1:776573/251‑1 (MQ=255)                                                                                                                                                                                                  ttGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTgaga                                             <  1:860843/251‑1 (MQ=255)                                                                                                                                                                                                  ttGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTgaga                                             <  1:172879/251‑1 (MQ=255)                                                                                                                                                                                                   tGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGAt                                            <  1:259678/251‑1 (MQ=255)                                                                                                                                                                                                            ttcttttcCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCAttt                                   <  1:759166/251‑1 (MQ=255)                                                                                                                                                                                                             tcttttcCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATcc                                                                                                                                                                                       <  1:813470/102‑1 (MQ=255)                                                                                                                                                                                                                  tcCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCg                             <  1:257799/251‑1 (MQ=255)                                                                                                                                                                                                                  tcCCAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCg                             >  1:385859/1‑251 (MQ=255)                                                                                                                                                                                                                   cccAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCAttt                                   <  1:150737/244‑1 (MQ=255)                                                                                                                                                                                                                   cccAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCg                             >  1:748564/1‑250 (MQ=255)                                                                                                                                                                                                                   cccAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCg                             >  1:363569/1‑250 (MQ=255)                                                                                                                                                                                                                   cccAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCg                             >  1:502300/1‑250 (MQ=255)                                                                                                                                                                                                                   cccAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCg                             >  1:392959/1‑250 (MQ=255)                                                                                                                                                                                                                     cAATAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCa                          >  1:584945/1‑251 (MQ=255)                                                                                                                                                                                                                        tAACTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGAGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTgcgc                                                             >  1:429480/1‑213 (MQ=255)                                                                                                                                                                                                                         aaCTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAgg                        >  1:176298/1‑249 (MQ=255)                                                                                                                                                                                                                          aCTGCGGTTGTAATTGAATAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTg                     <  1:706507/251‑1 (MQ=255)                                                                                                                                                                                                                                gTTGTAATTGAATAAAGTTGCTGTCCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCAc                                                    >  1:640088/1‑214 (MQ=255)                                                                                                                                                                                                                                           aTAAAGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGcc    <  1:130738/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaaGTTGCTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCtt  >  1:219421/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     CATATCGGCTTTTTGTGCGGCATTAAGCAAGCAATTTTTAACCCGAATTTATTCCCGTAGGGAAATATGACAGGCAGTTAACCTAATTCCGGATCTTGATCCTGAAATTGAAAATTCGGCGGCAGTTGCGGGCTGGCGTCATCTTCGCCCAGCGGGTCCACTTCCGGCGTGCGTTTAAACTTCTCCGGTGAGTTTGCATTTTTTTCTTTTCCCAATAACTGCGGTTGTAATTGAATAAAGTTACTGTTCGGCGACAACCAAATGCTAATCAGCGCTTGTGCAAATTGCGGTTCGAATTCATGATCCAAAATGGTGTCGTTTAAAATGAAATAGCTTTTTTCCGGCAGGGCGACAAAACGCAAAATGTCGTTAGGTGAGAAGTCGGGAAAAAGCTGCTGCATTTGCTTCAACCACAAATCGCCTTGCGCTTTATCCACGTTGAGATGTTCCATTTCTTTCGCCAGGCTGATGGCAAAATTTTTGCCTT  >  1/1164743‑1165229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑