Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,159,627	A→G	T251A (ACA→GCA)	unknown →	L‑xylulose 5‑phosphate 3‑epimerase (EC 5.1.3.‑)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,159,627	0	A	G	100.0%	181.1 / NA	64	T251A (ACA→GCA)	unknown	L‑xylulose 5‑phosphate 3‑epimerase (EC 5.1.3.‑)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/22);  total (42/22)

GACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCTTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGGACATTGGCAGAATTGAATTATCGCGGCACATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATAAAACCTTCCGGCGTGGAA  >  1/1159387‑1159871                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     gACCTTATCAAATCGCCGTGGTTCACTATGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAAt                                                                                                                                                                                                                                            >  1:6524/1‑251 (MQ=255)  aCCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAAtt                                                                                                                                                                                                                                           >  1:704076/1‑251 (MQ=255)      tATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGaaa                                                                                                                                                                                                                                       >  1:551888/1‑251 (MQ=255)      tATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGaaa                                                                                                                                                                                                                                       >  1:503817/1‑251 (MQ=255)                ccGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGa                                                                                                                                                                                                                             >  1:889631/1‑251 (MQ=255)                    ggTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGaaaaa                                                                                                                                                                                                                         >  1:65405/1‑251 (MQ=255)                     gTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAg                                                                                                                                                                                                                        >  1:886012/1‑251 (MQ=255)                          cTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTaa                                                                                                                                                                                                                                             >  1:144514/1‑225 (MQ=255)                          cTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGgaa                                                                                                                                                                                                                   >  1:185012/1‑251 (MQ=255)                          cTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGgaa                                                                                                                                                                                                                   >  1:364021/1‑251 (MQ=255)                              gtATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAAtt                                                                                                                                                                                                                                           <  1:651378/223‑1 (MQ=255)                              gtATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGgaagaa                                                                                                                                                                                                                >  1:143438/1‑250 (MQ=255)                                aTCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCg                                                                                                                                                                                                             >  1:69137/1‑251 (MQ=255)                                     gATCTCGACAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAATCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTg                                                                                                                                                                                                         >  1:303751/1‑250 (MQ=255)                                               aTTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAattatt                                                                                                                                                                                              >  1:891566/1‑251 (MQ=255)                                               aTTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAattatt                                                                                                                                                                                              <  1:511764/251‑1 (MQ=255)                                                   aaGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAAcg                                                                                                                                                                                          <  1:177400/251‑1 (MQ=255)                                                      tGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAAcgcc                                                                                                                                                                                        <  1:718928/250‑1 (MQ=255)                                                       gCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAAcgcccg                                                                                                                                                                                      >  1:822470/1‑251 (MQ=255)                                                            gAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGtt                                                                                                                                                                                 >  1:252017/1‑251 (MQ=255)                                                               tGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGa                                                                                                                                                                              >  1:297495/1‑251 (MQ=255)                                                                 aCAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGAt                                                                                                                                                                             <  1:77129/250‑1 (MQ=255)                                                                 aCAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGAt                                                                                                                                                                             <  1:703057/250‑1 (MQ=255)                                                                                     aaGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAgg                                                                                                                                                        <  1:427405/251‑1 (MQ=255)                                                                                       gCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAggtg                                                                                                                                                      >  1:229292/1‑251 (MQ=255)                                                                                             cATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTc                                                                                                                                                <  1:762698/251‑1 (MQ=255)                                                                                                 gACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATg                                                                                                                                            >  1:344112/1‑251 (MQ=255)                                                                                                  aCAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAATATGAAAGAAGGTGGTTTTCCATg                                                                                                                                            <  1:481212/250‑1 (MQ=255)                                                                                                                 ttcattTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAg                                                                                                                            <  1:474283/251‑1 (MQ=255)                                                                                                                              cacTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTc                                                                                                                                                <  1:773520/218‑1 (MQ=255)                                                                                                                                cTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAg                                                                                                             >  1:297072/1‑251 (MQ=255)                                                                                                                                cTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAg                                                                                                             >  1:158538/1‑251 (MQ=255)                                                                                                                                cTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAg                                                                                                             >  1:445332/1‑251 (MQ=255)                                                                                                                                        gTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAAt                                                                                                        >  1:48/1‑248 (MQ=255)                                                                                                                                           aCCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCg                                                                                                  <  1:891543/251‑1 (MQ=255)                                                                                                                                           aCCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCg                                                                                                  >  1:198979/1‑251 (MQ=255)                                                                                                                                                cacCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTAc                                                                                              >  1:217173/1‑250 (MQ=255)                                                                                                                                                     gCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAGCAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGaaa                                                                                         >  1:452457/1‑250 (MQ=255)                                                                                                                                                     gCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAAt                                                                                        >  1:586801/1‑251 (MQ=255)                                                                                                                                                          gggCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGaa                                                                                                                          <  1:800742/212‑1 (MQ=255)                                                                                                                                                          gggCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAAt                                                                                   >  1:572544/1‑251 (MQ=255)                                                                                                                                                           gtcAATTCCGTAACGTGCAGTTCGGTGACGGTGGTGTGGATTTTGTTGCCTGTTTAAGAACATTGGCAGAATTGAATTATCGCGGCGAATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGACCCGATTGCGAAAATTATTAAGGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAAt                                                                                   <  1:687301/248‑1 (MQ=255)                                                                                                                                                                          tGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACtt                                                                   <  1:472249/251‑1 (MQ=255)                                                                                                                                                                             cgttCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAac                                                                                                                                    >  1:864314/3‑183 (MQ=255)                                                                                                                                                                                 gcgtgtcggaagacggttttttGCTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCaa                                                            >  1:543610/19‑251 (MQ=255)                                                                                                                                                                                  ggTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACaaaaa                                                                                                                                                                   <  1:140187/147‑1 (MQ=255)                                                                                                                                                                                  ggTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATATTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCaa                                                            >  1:443370/1‑250 (MQ=255)                                                                                                                                                                                     gACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGtt                                                        >  1:208723/1‑251 (MQ=255)                                                                                                                                                                                       cGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAg                                                                                                                      >  1:83542/1‑187 (MQ=255)                                                                                                                                                                                            gtgtgGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCa                                                 >  1:352887/1‑251 (MQ=255)                                                                                                                                                                                                 gATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGAt                                            >  1:607247/1‑251 (MQ=255)                                                                                                                                                                                                            cTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGaaa                                                                                                                                     >  1:71721/1‑151 (MQ=255)                                                                                                                                                                                                               tttCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTgggg                                                               <  1:78549/218‑1 (MQ=255)                                                                                                                                                                                                               tttCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAAt                                >  1:465996/1‑249 (MQ=255)                                                                                                                                                                                                                     aaCATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTggtggt                        >  1:419616/2‑251 (MQ=255)                                                                                                                                                                                                                         ttGGCAGAATGGAATTATCAGGGCGATTTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGACCCGATTGGGAAAATTATTAACGCGCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATa                    <  1:864274/251‑1 (MQ=255)                                                                                                                                                                                                                               gaattgaattATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATAAAAc                >  1:462559/1‑249 (MQ=255)                                                                                                                                                                                                                                aattgaattATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATAAAAc                <  1:729109/248‑1 (MQ=255)                                                                                                                                                                                                                                 attgaattATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATAAAACCTTc            <  1:236223/251‑1 (MQ=255)                                                                                                                                                                                                                                    gaattATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGc                                                                                                            >  1:385059/1‑152 (MQ=255)                                                                                                                                                                                                                                    gaattATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATAAAACCTTCCgg         >  1:354828/1‑251 (MQ=255)                                                                                                                                                                                                                                    gaattATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGACTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATAGCGAAAAAAATCTGGTTGTGATAAAAcatgtct          >  1:304956/1‑244 (MQ=255)                                                                                                                                                                                                                                     aattATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATAAAACCTTCCgg         <  1:835090/250‑1 (MQ=255)                                                                                                                                                                                                                                           cgcgGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATAAAACCTTCCGGCGTGGaa  <  1:637973/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     GACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCTTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGGACATTGGCAGAATTGAATTATCGCGGCACATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAATCTGGTGGTGATAAAACCTTCCGGCGTGGAA  >  1/1159387‑1159871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑