Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,159,599	G→A	R241R (AGG→AGA)	unknown →	L‑xylulose 5‑phosphate 3‑epimerase (EC 5.1.3.‑)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,159,599	0	G	A	100.0%	171.6 / NA	62	R241R (AGG→AGA)	unknown	L‑xylulose 5‑phosphate 3‑epimerase (EC 5.1.3.‑)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/22);  total (40/22)

GCTCCATCAGTCGCTGGAAAAAATGGGATGACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCTTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGGACATTGGCAGAATTGAATTATCGCGGCACATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAAT  >  1/1159358‑1159841                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   gCTCCATCAGTCGCTGGAAAAAATGGGATGACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGc                                                                                                                                                                                                                                            <  1:641004/250‑1 (MQ=255)     cATCAGTCGCTGGAAAAAATGGGATGACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAgaat                                                                                                                                                                                                                                       <  1:343910/251‑1 (MQ=255)       tCAGTCGCTGGAAAAAATGGGATGACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAgaattg                                                                                                                                                                                                                                     <  1:207022/251‑1 (MQ=255)           tCGCTGGAAAAAATGGGATGACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAgaattgaat                                                                                                                                                                                                                                  <  1:888751/250‑1 (MQ=255)                ggAAAAAATGGGATGACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAgaattgaat                                                                                                                                                                                                                                  >  1:97622/1‑245 (MQ=255)                              gACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATcg                                                                                                                                                                                                                             >  1:299130/1‑236 (MQ=255)                              gACCTTATCAAATCGCCGTGGTTCACTATGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAAt                                                                                                                                                                                                              >  1:6524/1‑251 (MQ=255)                               aCCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAgaattg                                                                                                                                                                                                                                     >  1:786929/1‑227 (MQ=255)                               aCCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAAtt                                                                                                                                                                                                             >  1:704076/1‑251 (MQ=255)                                   tATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGaaa                                                                                                                                                                                                         >  1:503817/1‑251 (MQ=255)                                   tATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGaaa                                                                                                                                                                                                         >  1:551888/1‑251 (MQ=255)                                             ccGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGa                                                                                                                                                                                               >  1:889631/1‑251 (MQ=255)                                                 ggTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTa                                                                                                                                                                                                                                <  1:405551/214‑1 (MQ=255)                                                 ggTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGaaaaa                                                                                                                                                                                           >  1:65405/1‑251 (MQ=255)                                                  gTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAg                                                                                                                                                                                          >  1:886012/1‑251 (MQ=255)                                                       cTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTaa                                                                                                                                                                                                               >  1:144514/1‑225 (MQ=255)                                                       cTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGgaa                                                                                                                                                                                     >  1:185012/1‑251 (MQ=255)                                                       cTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGgaa                                                                                                                                                                                     >  1:364021/1‑251 (MQ=255)                                                           gtATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAAtt                                                                                                                                                                                                             <  1:651378/223‑1 (MQ=255)                                                           gtATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGgaagaa                                                                                                                                                                                  >  1:143438/1‑250 (MQ=255)                                                             aTCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCg                                                                                                                                                                               >  1:69137/1‑251 (MQ=255)                                                                  gATCTCGACAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAATCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTg                                                                                                                                                                           >  1:303751/1‑250 (MQ=255)                                                                            aTTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAattatt                                                                                                                                                                >  1:891566/1‑251 (MQ=255)                                                                            aTTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAattatt                                                                                                                                                                <  1:511764/251‑1 (MQ=255)                                                                                aaGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAAcg                                                                                                                                                            <  1:177400/251‑1 (MQ=255)                                                                                   tGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAAcgcc                                                                                                                                                          <  1:718928/250‑1 (MQ=255)                                                                                    gCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAAcgcccg                                                                                                                                                        >  1:822470/1‑251 (MQ=255)                                                                                         gAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGtt                                                                                                                                                   >  1:252017/1‑251 (MQ=255)                                                                                            tGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGa                                                                                                                                                >  1:297495/1‑251 (MQ=255)                                                                                              aCAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGAt                                                                                                                                               <  1:77129/250‑1 (MQ=255)                                                                                              aCAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGAt                                                                                                                                               <  1:703057/250‑1 (MQ=255)                                                                                                                  aaGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAgg                                                                                                                          <  1:427405/251‑1 (MQ=255)                                                                                                                    gCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAggtg                                                                                                                        >  1:229292/1‑251 (MQ=255)                                                                                                                          cATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTc                                                                                                                  <  1:762698/251‑1 (MQ=255)                                                                                                                              gACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATg                                                                                                              >  1:344112/1‑251 (MQ=255)                                                                                                                               aCAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAATATGAAAGAAGGTGGTTTTCCATg                                                                                                              <  1:481212/250‑1 (MQ=255)                                                                                                                                              ttcattTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAg                                                                                              <  1:474283/251‑1 (MQ=255)                                                                                                                                                           cacTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTc                                                                                                                  <  1:773520/218‑1 (MQ=255)                                                                                                                                                             cTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAg                                                                               >  1:297072/1‑251 (MQ=255)                                                                                                                                                             cTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAg                                                                               >  1:158538/1‑251 (MQ=255)                                                                                                                                                             cTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAg                                                                               >  1:445332/1‑251 (MQ=255)                                                                                                                                                                     gTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAAt                                                                          >  1:48/1‑248 (MQ=255)                                                                                                                                                                        aCCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCg                                                                    >  1:198979/1‑251 (MQ=255)                                                                                                                                                                        aCCGACACCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCg                                                                    <  1:891543/251‑1 (MQ=255)                                                                                                                                                                             cacCTGCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTAc                                                                >  1:217173/1‑250 (MQ=255)                                                                                                                                                                                  gCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAGCAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGaaa                                                           >  1:452457/1‑250 (MQ=255)                                                                                                                                                                                  gCAAAGGGCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAAt                                                          >  1:586801/1‑251 (MQ=255)                                                                                                                                                                                       gggCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGaa                                                                                            <  1:800742/212‑1 (MQ=255)                                                                                                                                                                                       gggCAATTCCGTGACGTGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAAt                                                     >  1:572544/1‑251 (MQ=255)                                                                                                                                                                                        gtcAATTCCGTAACGTGCAGTTCGGTGACGGTGGTGTGGATTTTGTTGCCTGTTTAAGAACATTGGCAGAATTGAATTATCGCGGCGAATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGACCCGATTGCGAAAATTATTAAGGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAAt                                                     <  1:687301/248‑1 (MQ=255)                                                                                                                                                                                                       tGCCGTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACtt                                     <  1:472249/251‑1 (MQ=255)                                                                                                                                                                                                          cgttCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAac                                                                                                      >  1:864314/3‑183 (MQ=255)                                                                                                                                                                                                              gcgtgtcggaagacggttttttGCTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCaa                              >  1:543610/19‑251 (MQ=255)                                                                                                                                                                                                               ggTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACaaaaa                                                                                                                                     <  1:140187/147‑1 (MQ=255)                                                                                                                                                                                                               ggTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATATTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCaa                              >  1:443370/1‑250 (MQ=255)                                                                                                                                                                                                                  gACGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGtt                          >  1:208723/1‑251 (MQ=255)                                                                                                                                                                                                                    cGGTTGTGTGGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAg                                                                                        >  1:83542/1‑187 (MQ=255)                                                                                                                                                                                                                         gtgtgGATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCa                   >  1:352887/1‑251 (MQ=255)                                                                                                                                                                                                                              gATTTTGTTGCCTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGAt              >  1:607247/1‑251 (MQ=255)                                                                                                                                                                                                                                         cTGTTTCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGaaa                                                                                                       >  1:71721/1‑151 (MQ=255)                                                                                                                                                                                                                                            tttCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTgggg                                 <  1:78549/218‑1 (MQ=255)                                                                                                                                                                                                                                            tttCAGAACATTGGCAGAATTGAATTATCGCGGCGCATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAAt  >  1:465996/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   GCTCCATCAGTCGCTGGAAAAAATGGGATGACCTTATCAAATCGCCGTGGTTCACTGTGTATCCGGATCTCGGCAATTTAAGTGCGTGGAATGACAACGTCGCGAAAGAATTGAAGCTGGGCATCGACAAAATCTCCGCCATTCATTTGAAAGACACTTATAAAGTTACCGACACCTGCAAAGGGCAATTCCGTGACGTGCCTTTCGGTGACGGTTGTGTGGATTTTGTTGCCTGTTTCAGGACATTGGCAGAATTGAATTATCGCGGCACATTTTTAATTGAAATGTGGACGGAAAAAGCGGAAGAACCGATTGCGGAAATTATTAACGCCCGCCGTTGGATTGAACAAAAAATGAAAGAAGGTGGTTTTCCATGTTAGAAACACTTAAAGAAAAAGTCTTAAAAGCAAATCTGGCGTTACCGAAATACAATTTGGTGACTTTCACTTGGGGCAACGTTTCCGCCATTGATCGCGAAAAAAAT  >  1/1159358‑1159841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑