Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,138,296	T→C	I115V (ATC→GTC)	unknown ←	Cys‑tRNA(Pro) deacylase YbaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,138,296	0	T	C	100.0%	171.1 / NA	59	I115V (ATC→GTC)	unknown	Cys‑tRNA(Pro) deacylase YbaK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/26);  total (33/26)

CAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGATGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCGTTTTCCGCAACCAATAAAGTCTTGAAAGATTGATCGGGATTTATGCCTAATTTCTCG  >  1/1138078‑1138537                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  cAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATggg                                                                                                                                                                                                                    >  1:639217/1‑250 (MQ=255)  aGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATggg                                                                                                                                                                                                                    >  1:131147/1‑249 (MQ=255)    cTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGc                                                                                                                                                                                                                   <  1:230377/248‑1 (MQ=255)      ataCTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGAtt                                                                                                                                                                                                              <  1:202184/251‑1 (MQ=255)       taCTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTc                                                                                                                                                                                                             <  1:472404/251‑1 (MQ=255)                                   aGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTtg                                                                                                                                                                                 >  1:182243/1‑251 (MQ=255)                                                             aGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACttttt                                                                                                                                                                                   <  1:353709/223‑1 (MQ=255)                                                                  aaTTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTAc                                                                                                                                                                                        <  1:108165/213‑1 (MQ=255)                                                                            gCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCa                                                                                                                                        >  1:647011/1‑251 (MQ=255)                                                                               cTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCg                                                                                                                                                                                                                                       >  1:872660/1‑153 (MQ=255)                                                                               cTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAg                                                                                                                                                                                                  <  1:260044/190‑1 (MQ=255)                                                                               cTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGc                                                                                                                                                                              <  1:357543/210‑1 (MQ=255)                                                                               cTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTcc                                                                                                                                                                 >  1:212132/1‑223 (MQ=255)                                                                               cTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCAc                                                                                                                                                      >  1:267013/1‑234 (MQ=255)                                                                               cTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAgg                                                                                                                                     >  1:507972/1‑251 (MQ=255)                                                                               cTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAgg                                                                                                                                     >  1:34324/1‑251 (MQ=255)                                                                               cTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAgg                                                                                                                                     >  1:802964/1‑251 (MQ=255)                                                                                 tttACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAgg                                                                                                                                     >  1:622535/1‑249 (MQ=255)                                                                                     ccccTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCg                                                                                                                                                                                                                                       <  1:505569/147‑1 (MQ=255)                                                                                      cccTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCt                                                                                                                                   <  1:545186/246‑1 (MQ=255)                                                                                        cTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTAc                                                                                                                                                                                        <  1:398583/191‑1 (MQ=255)                                                                                        cTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCg                                                                                                                            >  1:765730/1‑251 (MQ=255)                                                                                          cATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCg                                                                                                                            >  1:619597/1‑249 (MQ=255)                                                                                           aTCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGc                                                                                                                                                                                                                   <  1:417046/161‑1 (MQ=255)                                                                                             ccACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCtttt                                                                                                                       >  1:183688/1‑251 (MQ=255)                                                                                                  atatCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAg                                                                                                                  >  1:241060/1‑251 (MQ=255)                                                                                                    atCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAgg                                                                                                                 >  1:576235/1‑250 (MQ=255)                                                                                                    atCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAgg                                                                                                                 >  1:168495/1‑250 (MQ=255)                                                                                                    atCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAgg                                                                                                                 >  1:796611/1‑250 (MQ=255)                                                                                                     tCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAgg                                                                                                                 >  1:862579/1‑249 (MQ=255)                                                                                                           aaCGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAg                                                                                                         <  1:472333/251‑1 (MQ=255)                                                                                                                cGCATTCAATATTTTCGCCAAATCCCGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGc                                                                                                    <  1:728116/251‑1 (MQ=255)                                                                                                                   aTTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCtgg                                                                                                 <  1:19078/251‑1 (MQ=255)                                                                                                                      cAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCtggtgg                                                                                              >  1:132613/1‑251 (MQ=255)                                                                                                                        atatTTGCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCGGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCACGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGgc                                                                                            <  1:12640/251‑1 (MQ=255)                                                                                                                          atTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGgcag                                                                                          >  1:804449/1‑251 (MQ=255)                                                                                                                          atTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGgcag                                                                                          >  1:413221/1‑251 (MQ=255)                                                                                                                                 ccAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGaaa                                                                                   >  1:725595/1‑251 (MQ=255)                                                                                                                                  cAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAAc                                                                                  <  1:33088/251‑1 (MQ=255)                                                                                                                                     aTCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCTCTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAgg                                                                               <  1:879465/251‑1 (MQ=255)                                                                                                                                     aTCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAgg                                                                               >  1:896423/1‑251 (MQ=255)                                                                                                                                                cGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTc                                                                    <  1:663000/251‑1 (MQ=255)                                                                                                                                                  aTTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAg                                                                                                         <  1:3990/212‑1 (MQ=255)                                                                                                                                                  aTTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCttttt                                                                     >  1:275776/1‑248 (MQ=255)                                                                                                                                                  aTTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCttttt                                                                     >  1:426289/1‑248 (MQ=255)                                                                                                                                                   tttCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAAc                                                                                  <  1:339986/234‑1 (MQ=255)                                                                                                                                                      cAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAg                                                                                                         <  1:106304/208‑1 (MQ=255)                                                                                                                                                        aCGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGcc                                                            <  1:456867/251‑1 (MQ=255)                                                                                                                                                         cGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCg                                                           >  1:479244/1‑251 (MQ=255)                                                                                                                                                                   gcgTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAg                                                                                                         <  1:153447/195‑1 (MQ=255)                                                                                                                                                                             ccgGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCGTTTTCCGCAACCAATAAAg                                        <  1:27652/250‑1 (MQ=255)                                                                                                                                                                                  gaCGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGc                                                                          <  1:869061/211‑1 (MQ=255)                                                                                                                                                                                  gaCGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTc                                                                    >  1:566420/1‑217 (MQ=255)                                                                                                                                                                                                       cAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCGTTTTCCGCAACCAATAAAGTCTTGAAAGATTGATCGGGATTTATg              >  1:171500/1‑250 (MQ=255)                                                                                                                                                                                                              gATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCAc                                                                                       >  1:815467/1‑170 (MQ=255)                                                                                                                                                                                                              gATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCGTTTTCCGCAACCAATAAAGTCTTGAAAGATTGATCGGGATTTATGCCTAAttt      >  1:361629/1‑251 (MQ=255)                                                                                                                                                                                                              gATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCGTTTTCCGCAACCAATAAAGTCTTGAAAGATTGATCGGGATTTATGCCTAAt        <  1:412937/249‑1 (MQ=255)                                                                                                                                                                                                                  gTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCGTTTTCCGCAACCAATAAAGTCTTGAAAGATTGATCGGGATTTATGCCTAATTTCTcg  >  1:820079/1‑251 (MQ=255)                                                                                                                                                                                                                  gTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCGTTTTCCGCAACCAATAAAGTCTTGAAAGATTGATCGGGATTTATGCCTAATTTCTcg  >  1:735563/1‑251 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  CAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGATGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTCCGCCCACCAAGTAGCCGGTACTTTTTTGTGCCGCGTCTTTGTCCGCCATTTCCACTTTTTTGACGCCCAAGGCTTTTGCCGCTTTTTTCAGGCTTAACAGATGGCTGGTGGGCAGCACGAAACAGGCGAGCTTTTTCTGATCGCCGTTTTCCGCAACCAATAAAGTCTTGAAAGATTGATCGGGATTTATGCCTAATTTCTCG  >  1/1138078‑1138537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑