Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,110,606	C→T	G113E (GGA→GAA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,110,606	0	C	T	100.0%	41.5 / NA	16	G113E (GGA→GAA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/6);  total (10/6)

ATTTTCAATGTATATTTCGAATAGTTCTTTATCTTCATGAAAGGAATTTACAATACCCCTAATAAATAAATTTTTATCCCAAATAGTGATATCTATGTATTGATCTTTGTTATCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATCCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGAATTAACATCCCATACTTGAAGATACTCTGCATTATTTAATGTGAAATTTTCGTACCCGATTTTGGCACATTGATTTAATAGCGATAGAATTTGTAATATTAAGTAAGATAATATTCCATATGAAATAACAAACAATGAATATTTATAGTTATTC  >  1/1110413‑1110851                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      aTTTTCAATGTATATTTCGAATAGTTCTTTATCTTCATGAAAGGAATTTACAATACCCCTAATAAATAAATTTTTATCCCAAATAGTGATATCTATGTATTGATCTTTGTTATCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAg                                                                                                                                                                                              <  1:481374/251‑1 (MQ=255)                                            gAATTTACAATACCCCTAATAAATAAATTTTTATCCCAAATAGTGATATCTATGTATTGATCTTTGTTATCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACtt                                                                                                                                                                                     <  1:12317/217‑1 (MQ=255)                                                                        ttttATCCCAAATAGTGATATCTATGTATTGATCTTTGTTATCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGGAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGAATTAACATCCCATACTTGAAGATACTCTGTATTAttt                                                                                                                       <  1:758324/251‑1 (MQ=255)                                                                                            tCTATGTATTGATCTTTGTTATCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAaggt                                                                                                                                                                          >  1:318570/1‑180 (MQ=255)                                                                                            tCTATGTATTGATCTTTGTTATCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAaggt                                                                                                                                                                          >  1:753791/1‑180 (MQ=255)                                                                                            tCTATGTATTGATCTTTGTTATCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGAATTAACATCCCATACTTGAAGATACTCTGTATTATTTAATGTGAAATTTTCGTAcc                                                                                                    >  1:99653/1‑250 (MQ=255)                                                                                                                aTCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTc                                                                                                                                                                                                    >  1:116902/1‑134 (MQ=255)                                                                                                                               ggggAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAAc                                                                                                                                                                                                                                    >  1:484477/1‑87 (MQ=255)                                                                                                                               ggggAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGc                                                                                                                                                                                             >  1:672971/1‑126 (MQ=255)                                                                                                                                       cTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACtt                                                                                                                                                              >  1:462844/1‑149 (MQ=255)                                                                                                                                       cTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACt                                                                                                                                                               >  1:526090/1‑148 (MQ=255)                                                                                                                                         ccATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGAATTAACATCCCATACTTGAAGATACTCTGTATTAttt                                                                                                                       <  1:744004/186‑1 (MQ=255)                                                                                                                                                      atTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGaa                                                                                                                                                           <  1:9959/137‑1 (MQ=255)                                                                                                                                                      atTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGAATTAACATCCCATACTTGAAGATACTCTGTATTATTTAATGTGAAATTTTCGTACCCGATTTTGGCACATTGATTTAATAGCGATAGAATTTGTAATATTAAGTAAGATAATAtt                                         >  1:182332/1‑251 (MQ=255)                                                                                                                                                                                              gTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGAATTAACATCCCATACTTGAAGATACTCTGTATTATTTAATGTGAAATTTTCGTACCCGATTTTGGCACATTGATTTAATAg                                                                           <  1:870053/177‑1 (MQ=255)                                                                                                                                                                                              gTATTCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGAATTAACATCCCATACTTGAAGATACTCTGTATTATTTAATGTGAAATTTTCGTACCCGATTTTGGCACATTGATTTAATAGCGATAGAATTTGTAATATTAAGTAAGATAATATTCCATATGAAATAACAAACAATGAATATTTATAGTTATTc  >  1:544605/1‑250 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ATTTTCAATGTATATTTCGAATAGTTCTTTATCTTCATGAAAGGAATTTACAATACCCCTAATAAATAAATTTTTATCCCAAATAGTGATATCTATGTATTGATCTTTGTTATCTTTTAATAACTGGGGGAATACTCCATAATCACCATATTTTCTAGAAATATTAAATTTAAGAAGAAATTTGGCAAGGTATCCTTTGTGATCTATGTAAACAAATAATAAACCAAGAAAAAATGACACTATTCCTGAAGCAATTACTTCATTATAAGGTAGGTTAATACTTGAATTAACATCCCATACTTGAAGATACTCTGCATTATTTAATGTGAAATTTTCGTACCCGATTTTGGCACATTGATTTAATAGCGATAGAATTTGTAATATTAAGTAAGATAATATTCCATATGAAATAACAAACAATGAATATTTATAGTTATTC  >  1/1110413‑1110851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑