| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,102,037 | G→A | A206A (GCG→GCA) | unknown → | Alanine racemase (EC 5.1.1.1) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,102,037 | 0 | G | A | 98.8% | 246.0 / NA | 86 | A206A (GCG→GCA) | unknown | Alanine racemase (EC 5.1.1.1) |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (54/31); total (54/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CCGGTATGCACCGTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCGCATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATCGGCTACGGCGACGGTTATCCGCGTGACGTGCCGGAAGG > 1/1101802‑1102276 | ccGGTATGCACCGTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACAt > 1:703487/1‑239 (MQ=255) ggTATGCACCGTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCg > 1:808678/1‑251 (MQ=255) ggTATGCACCGTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCg > 1:730456/1‑251 (MQ=255) ggTATGCACCGTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCg > 1:330873/1‑251 (MQ=255) aTGCACCGTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGccc < 1:711699/251‑1 (MQ=255) gTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtatt > 1:611344/1‑251 (MQ=255) gTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtatt > 1:537637/1‑251 (MQ=255) gTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGAAGCACATTTGGATTGGATCCGCCCTGGtatt > 1:611110/1‑251 (MQ=255) ggggTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta > 1:469424/1‑250 (MQ=255) gggTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta < 1:471469/249‑1 (MQ=255) ggTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGt > 1:899013/1‑251 (MQ=255) cATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATttggat < 1:794643/222‑1 (MQ=255) cATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta < 1:558832/244‑1 (MQ=255) cATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACgg > 1:769961/1‑251 (MQ=255) cATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACgg > 1:493808/1‑251 (MQ=255) cATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACgg > 1:588350/1‑251 (MQ=255) cATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGGATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTggca > 1:145303/1‑236 (MQ=255) aTTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGc < 1:588154/251‑1 (MQ=255) gACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATttggatt > 1:25971/1‑217 (MQ=255) gACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCAttt > 1:609195/1‑250 (MQ=255) gACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCAttt > 1:838419/1‑250 (MQ=255) gTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta < 1:614606/232‑1 (MQ=255) gTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtatta < 1:299154/229‑1 (MQ=255) ccTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATAc < 1:687556/250‑1 (MQ=255) ttttATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATAccgc < 1:760007/251‑1 (MQ=255) tATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCgg < 1:494841/251‑1 (MQ=255) aaTTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGt > 1:634688/1‑216 (MQ=255) aaTCGTTCCCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGt < 1:741699/251‑1 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGGCGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGttt > 1:428084/1‑251 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta > 1:856962/1‑205 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta > 1:630344/1‑205 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta > 1:377963/1‑205 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGt > 1:669342/1‑208 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTAc < 1:780700/210‑1 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGttt > 1:487910/1‑251 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGttt < 1:67799/251‑1 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGttt > 1:160962/1‑251 (MQ=255) tCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGttt > 1:406067/1‑251 (MQ=255) ttACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAAt < 1:540527/251‑1 (MQ=255) ccccTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTc < 1:564299/250‑1 (MQ=255) aCCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCGTTTGGGTCGCGCCGATGAATTGGCATGCGGTTATCCCGAAAAACAATTGGCGCGTTTTTTACAGGGGACGGAGGGTAAAGGGGGTGAGCGTAGTATTCCCGCATCCGGCGGTATTTTATTTTGGCAGGACGCACATTTGGATTGGATCCGCCGTGGTATTATTATGTCCGGCATTTCACGGAATAATACCGCCGGCAAAGAATACGGTTTAATTc < 1:854825/251‑1 (MQ=255) aCCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTAc > 1:743236/1‑205 (MQ=255) aCCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATAc > 1:654188/1‑225 (MQ=255) gtgtTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta > 1:624568/1‑195 (MQ=255) gtgtTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCAc > 1:375970/1‑210 (MQ=255) gtgtTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATAc < 1:896142/220‑1 (MQ=255) gtgtTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTc > 1:754030/1‑246 (MQ=255) gtgtTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGtg > 1:532712/1‑251 (MQ=255) ctgtTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGtg > 1:742279/2‑251 (MQ=255) gAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtattatta < 1:592849/185‑1 (MQ=255) tatCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGt < 1:311341/251‑1 (MQ=255) tCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGAGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGCTTGGATCCGCCCTGGGATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGt > 1:438314/1‑249 (MQ=255) gTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCGCATTTGGATTGGATCCGCCCTGGtattatta < 1:243291/177‑1 (MQ=255) gTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCACATCCGGCGGTATTTTATTCTGGCAGGGCGCACATTTGGATTGGATCCGCCCTGGtattatta < 1:870532/172‑1 (MQ=255) ttAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAAt < 1:505468/250‑1 (MQ=255) ttttAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTa > 1:271635/1‑250 (MQ=255) ttttAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACAGCATTTCACCGAATAATACCGCCGGCAAAGAATACgg > 1:426968/1‑207 (MQ=255) gAATTGGCATGCGGTTATACCGAAAAGCAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGaataat < 1:66296/171‑1 (MQ=255) gAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAgg > 1:756163/1‑251 (MQ=255) gAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGCATTATTATGTACGGCATTTCACCGAATAATAc > 1:746841/1‑173 (MQ=255) cGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGc > 1:397988/1‑219 (MQ=255) cGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTgg > 1:353609/1‑251 (MQ=255) cGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTgg > 1:619427/1‑251 (MQ=255) ataCCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAGTACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTa > 1:451737/1‑250 (MQ=255) aaaaCAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGgcggcg < 1:587937/251‑1 (MQ=255) cAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTg > 1:155140/1‑251 (MQ=255) cgTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGa > 1:127685/1‑251 (MQ=255) gTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATc < 1:186055/95‑1 (MQ=255) gTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGtatt < 1:563067/107‑1 (MQ=255) gTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTc > 1:73683/1‑181 (MQ=255) aGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGc > 1:247805/1‑208 (MQ=255) ggTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGGTTGGATGAGTGACAAAGATACCAAAATCGGCgg > 1:420957/1‑250 (MQ=255) gTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAgg > 1:360264/1‑193 (MQ=255) gtgggCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAAt < 1:569726/246‑1 (MQ=255) tGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATc < 1:492611/250‑1 (MQ=255) gCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTAcggcgg > 1:230893/1‑201 (MQ=255) gCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACaa > 1:42903/1‑219 (MQ=255) gCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATc > 1:2330/1‑247 (MQ=255) gCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATCgg > 1:656324/1‑249 (MQ=255) gCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTACGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATCgg > 1:487312/1‑249 (MQ=255) gtaTTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGa < 1:480531/204‑1 (MQ=255) ccGCATCCGGCGGTATTTTATTCTGGCAGGCCGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATCGGCTACGGCGACGGt < 1:890705/251‑1 (MQ=255) aTTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATc < 1:131749/217‑1 (MQ=255) aTTCTGGCAGGACGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATCGGCTACGGCGACGGTTATCCGCGTGACGTGCCgg > 1:573424/1‑251 (MQ=255) ggCAGAACGCACATTTGGATTGGACCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATCGGCTACGGCGACGGTTATCCGCGTGACGTGCCGGAAgg < 1:783799/250‑1 (MQ=255) aCGCACATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATCGGCTACGGCGACGGTTATc > 1:806053/1‑225 (MQ=255) | CCGGTATGCACCGTTTAGGGGTACATTTAGACGAAGTGGATGCCTTTTATCAAAAATTAAAATCGTTACCCTGTGTTGACCCGAATATCGGTTTTGTTAGTCATTTTAGTCGCGCCGATGAATTGGCATGCGGTTATACCGAAAAACAATTGGCGCGTTTTTTACAGGCGACGGAAGGTAAAGGCGGTGAGCGTAGTATTTCCGCATCCGGCGGTATTTTATTCTGGCAGGACGCGCATTTGGATTGGATCCGCCCTGGTATTATTATGTACGGCATTTCACCGAATAATACCGCCGGCAAAGAATACGGTTTAATTCCGGTGATGAATCTTACGTCTTCCCTAATTGCCGTACGCGAACATAAAAAAGGCGAGCCGGTGGGCTACGGCGGCGTTTGGATGAGTGACAAAGATACCAAAATCGGCGTGGTGGCAATCGGCTACGGCGACGGTTATCCGCGTGACGTGCCGGAAGG > 1/1101802‑1102276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |