Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,091,342	T→G	V501V (GTT→GTG)	unknown →	Oligopeptide ABC transporter, substrate‑binding protein OppA (TC 3.A.1.5.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,091,342	0	T	G	100.0%	232.1 / NA	77	V501V (GTT→GTG)	unknown	Oligopeptide ABC transporter, substrate‑binding protein OppA (TC 3.A.1.5.1)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/50);  total (27/50)

CATTTTTAAGCCTGTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTTATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACAGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACGGATAATACGGTAATGGAACACTTTTTTGTAGTTTAAAGA  >  1/1091098‑1091583                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  cATTTTTAAGCCTGTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGAGCCCGCCAACCCGACGGAAGtgatttat                                                                                                                                                                                                                                             <  1:861843/251‑1 (MQ=255)   tttttAAGCCTGTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAg                                                                                                                                                                                                                                           <  1:453427/251‑1 (MQ=255)   tttttAAGCCTGTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAg                                                                                                                                                                                                                                           >  1:710056/1‑251 (MQ=255)    ggctacggctgtagaaCAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAg                                                                                                                                                                                                                                           >  1:514762/16‑250 (MQ=255)         aGCCTGTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGa                                                                                                                                                                                                                                     >  1:838016/1‑251 (MQ=255)              gTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAg                                                                                                                                                                                                                                           <  1:43640/240‑1 (MQ=255)              gTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGc                                                                                                                                                                                                                                          >  1:62819/1‑241 (MQ=255)              gTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGt                                                                                                                                                                                                                                >  1:240276/1‑251 (MQ=255)              gTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGt                                                                                                                                                                                                                                >  1:639576/1‑251 (MQ=255)              gTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGt                                                                                                                                                                                                                                >  1:562086/1‑251 (MQ=255)              gTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGt                                                                                                                                                                                                                                >  1:800045/1‑251 (MQ=255)               ttCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAg                                                                                                                                                                                                                                           <  1:696066/239‑1 (MQ=255)               ttCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGtt                                                                                                                                                                                                                               >  1:169337/1‑251 (MQ=255)                tCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGttt                                                                                                                                                                                                                              <  1:51960/251‑1 (MQ=255)                      gCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGtgatt                                                                                                                                                                                                                                                <  1:283644/227‑1 (MQ=255)                      gCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGttt                                                                                                                                                                                                                              <  1:296179/245‑1 (MQ=255)                      gCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAg                                                                                                                                                                                                                        >  1:540970/1‑251 (MQ=255)                          tAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGttt                                                                                                                                                                                                                              <  1:190895/241‑1 (MQ=255)                           aGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAACCCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGt                                                                                                                                                                                                                      >  1:773912/1‑249 (MQ=255)                              ccGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATa                                                                                                                                                                                                                <  1:311867/251‑1 (MQ=255)                               cGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATaa                                                                                                                                                                                                               <  1:543489/251‑1 (MQ=255)                               cGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATaa                                                                                                                                                                                                               <  1:449214/251‑1 (MQ=255)                               cGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATaa                                                                                                                                                                                                               <  1:143904/251‑1 (MQ=255)                                  aTAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAAtg                                                                                                                                                                                                             <  1:90025/250‑1 (MQ=255)                                  aTAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAAtg                                                                                                                                                                                                             <  1:280342/250‑1 (MQ=255)                                   tAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGGAAAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAAtgt                                                                                                                                                                                                            >  1:480806/1‑251 (MQ=255)                                    aaCAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGc                                                                                                                                                                                                          <  1:781908/251‑1 (MQ=255)                                      cAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAAtgtg                                                                                                                                                                                                           >  1:227872/1‑248 (MQ=255)                                              gTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAAtt                                                                                                                                                                                                >  1:190051/1‑251 (MQ=255)                                               ttATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTGCCGGCAGAACGCCCCGCACTTTACGGACGAATCGAGCAAATCCTGCCAGAGG‑AAAATGTGGTTCGGCCATTATACCAATCCACCGTTCCCGTCTATCTCAG‑CCCCACACTAAACGGATTCGCCCCTGCCAACCCGACGGAAGTGATTTTTAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAAtt                                                                                                                                                                                                <  1:283543/250‑1 (MQ=255)                                               ttATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAAtt                                                                                                                                                                                                <  1:884058/250‑1 (MQ=255)                                               ttATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAAtt                                                                                                                                                                                                <  1:539743/250‑1 (MQ=255)                                                  taGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGCCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGAt                                                                                                                                                                                                                 >  1:240965/1‑230 (MQ=255)                                                     tAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCCCCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACt                                                                                                                                                                                           <  1:178734/249‑1 (MQ=255)                                                          acgaACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGcc                                                                                                                                                                                    <  1:226413/251‑1 (MQ=255)                                                             aaCTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATa                                                                                                                                                                                                                <  1:700184/220‑1 (MQ=255)                                                                  gATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCATATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTtaataa                                                                                                                                                                             <  1:865730/250‑1 (MQ=255)                                                                      gCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATAc                                                                                                                                                                         <  1:647281/250‑1 (MQ=255)                                                                      gCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATAc                                                                                                                                                                         <  1:866736/250‑1 (MQ=255)                                                                       cTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTc                                                                                                                                                                       <  1:791298/251‑1 (MQ=255)                                                                         tATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAg                                                                                                                                                                                                                                           <  1:371397/181‑1 (MQ=255)                                                                         tATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATaa                                                                                                                                                                                                               >  1:459782/1‑209 (MQ=255)                                                                           tttGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAg                                                                                                                                                                   >  1:762734/1‑251 (MQ=255)                                                                            ttGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGc                                                                                                                                                                  <  1:635110/251‑1 (MQ=255)                                                                             tGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGcc                                                                                                                                                                 <  1:29646/251‑1 (MQ=255)                                                                             tGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGcc                                                                                                                                                                 <  1:274951/251‑1 (MQ=255)                                                                                      ccTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCCGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGaaa                                                                                                                                                         <  1:423974/250‑1 (MQ=255)                                                                                                   cTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTa                                                                                                                                                                                          >  1:41892/1‑204 (MQ=255)                                                                                                       cGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCttttt                                                                                                                                       <  1:548921/251‑1 (MQ=255)                                                                                                         gCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGc                                                                                                                                                                  >  1:55877/1‑222 (MQ=255)                                                                                                                      cacTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCat                                                                                                                        <  1:409797/251‑1 (MQ=255)                                                                                                                        cTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCatta                                                                                                                      >  1:818725/1‑251 (MQ=255)                                                                                                                           tACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTc                                                                                                                   <  1:1259/251‑1 (MQ=255)                                                                                                                                aCGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATAc                                                                                                              <  1:241873/251‑1 (MQ=255)                                                                                                                                aCGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATAc                                                                                                              <  1:16037/251‑1 (MQ=255)                                                                                                                                   aaTCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTAc                                                                                                                                                                                         <  1:640874/173‑1 (MQ=255)                                                                                                                                        aGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGt                                                                                                       <  1:757595/250‑1 (MQ=255)                                                                                                                                                   cAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAGATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTg                                                                                           <  1:626835/251‑1 (MQ=255)                                                                                                                                                      gagG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGaat                                                                                        >  1:37196/1‑251 (MQ=255)                                                                                                                                                       agG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGaat                                                                                        <  1:260608/250‑1 (MQ=255)                                                                                                                                                              aTGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATa                                                                                                                                                                                                                <  1:87493/124‑1 (MQ=255)                                                                                                                                                                               tatCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGc                                                                                                                                                                  >  1:556098/1‑153 (MQ=255)                                                                                                                                                                                  cAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAg                                                                                                                                                                                                                                           >  1:872277/1‑77 (MQ=255)                                                                                                                                                                                           gTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATAc                                                                                                              <  1:794415/193‑1 (MQ=255)                                                                                                                                                                                                 gTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTc                                                                                                                   <  1:475025/182‑1 (MQ=255)                                                                                                                                                                                                    tATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGa                                                                                                                                                           >  1:317705/1‑139 (MQ=255)                                                                                                                                                                                                     aTCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTAc                                          <  1:180511/251‑1 (MQ=255)                                                                                                                                                                                                      tctcAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACg                                         >  1:303877/1‑251 (MQ=255)                                                                                                                                                                                                       ctcAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATAc                                                                                                              >  1:682389/1‑181 (MQ=255)                                                                                                                                                                                                         cAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACGGat                                      <  1:559376/251‑1 (MQ=255)                                                                                                                                                                                                           a‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCAt                                                                                                  <  1:708786/189‑1 (MQ=255)                                                                                                                                                                                                                 acacTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACGGATAATACgg                               <  1:735204/251‑1 (MQ=255)                                                                                                                                                                                                                  cacTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACGGATAATACGGt                              <  1:888302/251‑1 (MQ=255)                                                                                                                                                                                                                    cTAAACGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACGGATAATACgg                               <  1:316089/248‑1 (MQ=255)                                                                                                                                                                                                                      aaaCGGCTACGACCCCGCCAACCCGACGGAAGTGATTTATAg                                                                                                                                                                                                                                           <  1:634395/42‑1 (MQ=255)                                                                                                                                                                                                                           gCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGc                                                                                                                                                                  <  1:898906/110‑1 (MQ=255)                                                                                                                                                                                                                           gCTACGACCCCGCCAACCCGACGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACt                                                                                                             >  1:479784/1‑163 (MQ=255)                                                                                                                                                                                                                                               aCGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACGGATAATACGGTAATGGAACACTTTTTTGTAGTTTAAAGa  <  1:8582/250‑1 (MQ=255)                                                                                                                                                                                                                                               aCGGAAGTGATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACGGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACGGATAATACGGTAATGGAACACTTTTTTGTAGTTTAAAGa  <  1:376911/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CATTTTTAAGCCTGTTCTACAGCCGTAGCCCGGATAACAAAAGCGGTTATAGTAACGACGAACTGGATGGCTTATTTGAACAAAGCCTGAAAGTCATTCTACCGGCAGAACGCACCGCACTTTACGGACGAATCGAGCAAATCCTGCAAGAGG‑AAAATGTGGTTCTGCCGTTATATCAATCCACCGTTCCCGTCTATCTCAA‑CCCCACACTAAACGGCTACGACCCCGCCAACCCGACGGAAGTTATTTATAGCAAGGATTTGTTTCGTAAGGTTAGATAATGTGCTGATAAAATTTAACTACATGCCTTAATAAATACTCAAAGCCTATTGAAATCGGTCAACAGGCTTTTTTATCCGGATATTCATTATTCCATACTTGACGTATCATTTAATTGAATAATCTTCTTAGTATAAAACAAAGCCAACGCCGGGTGGAAAATGTACGGATAATACGGTAATGGAACACTTTTTTGTAGTTTAAAGA  >  1/1091098‑1091583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑