Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,077,449	G→T	intergenic (‑366/+22)	unknown ← / ← unknown	hypothetical protein/Glycosyl transferase, family 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,077,449	0	G	T	100.0%	28.6 / NA	12	intergenic (‑366/+22)	unknown/unknown	hypothetical protein/Glycosyl transferase, family 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/7);  total (5/7)

GTATATTTTGAATTTACAAAAAAAGAGAATATTACGGCGGTACTGAATCCTATAAGGTTGCTTAATGACTGTACGTATGTTAACCGATAGAAAATATAAAATACTCCCCAGTGTATTATTGTATTTATGATACCTACTGAGAAGTATCGAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAATCCCCATGCCGGTAGCGAATACAGCAGTAGATATTTTTAGTACCGTACCAAGCTTGAGATACTGGAAGATAACGGAAGTACCGGTAATCAGTGAAAATAGTGTGCAAAT  >  1/1077301‑1077677                                                                                                                                                     |                                                                                                                                                                                                                                     gTATATTTTGAATTTATAAAAAAAGAGAATATTACGGCGGTACTGAATCCTATAAGGTTTCTTAATGACTGTACGTATGTTAACCGATAGAAAATATAAAATACTCCCCAGTGTATTATTTTATTTATGATACCTACTGAGAAGTATCTAAAGAATTGCTTTTTCATTTTTTTTTTAATATTTAGATAATTCATTCTTTTAAGTTCTTTTCTTTCATTGGATATTGAGTCTAGAATTAATGCAATTGCaaa                                                                                                                                >  1:380282/1‑251 (MQ=255)                                               aTCCTATAAGGTTGCTTAATGACTGTACGCATGTTAACCGATAGAAAATATAAAATACTCCCCAGTGTATTATTGTATTTATGATACCTACTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAAt                                                                                                              >  1:291635/1‑223 (MQ=255)                                                  cTATAAGGTTGCTTAATGACTGTACGTATGTTAACCGATAGAAAATATAAAATACTCCCCAGTGTATTATTGTATTTATGATACCTACTGAGAAGTATCTAAAGa                                                                                                                                                                                                                                 <  1:881592/105‑1 (MQ=255)                                                                                                   aaaTACTCCCCAGTGTATTATTGTATTTATGATACCTACTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTg                                                                                                                                                         <  1:272785/128‑1 (MQ=255)                                                                                                               gtgtATTATTGTATTTATGATACCTACTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAATCCCCATGCCGGTAGCGAATACAGCAGTAGATATTTTTAGTACCGTACCAAGCTTGAGATACTGGAAGATAACGGAAGTACCGGTAATCAGTg                  >  1:163927/1‑251 (MQ=255)                                                                                                                         gTATTTATGATACCTACTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAATCCCCATGCCGGTAGCGAATACAGCAGTAGATATTTTTAGTACCGTACCAAGCTTGAGATACTGGAAGATAACGGAAGTACCGGTAATCAGTGAAAATAg           >  1:687251/1‑248 (MQ=255)                                                                                                                                   tACCTACTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAATCCCCATGCCGGTAGCGAATAcagcag                                                                                    <  1:133546/165‑1 (MQ=255)                                                                                                                                      cTACTGAGAAGTATCTAAAGAATTGCTTTATCATTTtttattt                                                                                                                                                                                                           <  1:224773/43‑1 (MQ=255)                                                                                                                                      cTACTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGcc                                                                                                                   <  1:491827/131‑1 (MQ=255)                                                                                                                                        aCTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAATCCCCATGCCGGTAGCGAATACAGCAGTAGATATTTTTAGTACCGTACCAAGCTTGAGATACTgg                                              <  1:63276/198‑1 (MQ=255)                                                                                                                                        aCTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAATCCCCATGCCGGTAGCGAATACAGCAGTAGATATTTTTAGTACCGTACCAAGCTTGAGATACTGGAAGATAACGGAAGTACCGGTAATCAGTGAAAATAg           <  1:49835/233‑1 (MQ=255)                                                                                                                                        aCTGAGAAGTATCTAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAATCCCCATGCCGGTAGCGAATACAGCAGTAGATATTTTTAGTACCGTACCAAGCTTGAGATACTGGAAGATAACGGAAGTACCGGTAATCAGTGAAAATAGTGTGCAAAt  >  1:637523/1‑242 (MQ=255)                                                                                                                                                     |                                                                                                                                                                                                                                     GTATATTTTGAATTTACAAAAAAAGAGAATATTACGGCGGTACTGAATCCTATAAGGTTGCTTAATGACTGTACGTATGTTAACCGATAGAAAATATAAAATACTCCCCAGTGTATTATTGTATTTATGATACCTACTGAGAAGTATCGAAAGAATTGCTTTATCATTTTTTATTTAATATTGAGATAATTCATTCTTTTAAGTTCTTTTCTTGCATTGGATATTGAGTCTAGAATTAATGCAATTGCAAAAAATAATATAGCCAGAATCCCCATGCCGGTAGCGAATACAGCAGTAGATATTTTTAGTACCGTACCAAGCTTGAGATACTGGAAGATAACGGAAGTACCGGTAATCAGTGAAAATAGTGTGCAAAT  >  1/1077301‑1077677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑