Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,077,128	T→C	intergenic (‑45/+343)	unknown ← / ← unknown	hypothetical protein/Glycosyl transferase, family 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,077,128	0	T	C	100.0%	27.4 / NA	11	intergenic (‑45/+343)	unknown/unknown	hypothetical protein/Glycosyl transferase, family 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/5);  total (6/5)

TTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCACTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAATAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTCATTAAACCCATGAAGGAAACATAGAAAACCATTCGTGTAATATTAATTTCTTTTTCAAATGTATATTTTGAATTTACAAAAAAAGAGAATAT  >  1/1076905‑1077332                                                                                                                                                                                                                                |                                                                                                                                                                                                             ttttttAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATaa                                                                                                                                                                                            <  1:560144/242‑1 (MQ=255)                    atTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGaa                                                                                                                                                                >  1:842868/1‑251 (MQ=255)                                                                                  ttCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGAt                                                                                                   <  1:763812/250‑1 (MQ=255)                                                                                                   aGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTc                                                                                              >  1:5869/1‑238 (MQ=255)                                                                                                                                                                      aatTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATAc                                                                                                                                                                     >  1:118954/1‑100 (MQ=255)                                                                                                                                                                       atTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTCATTAAACCCATGAAGGAAACATAGAAAACCATTCGTGTAATATTAATTTCTTTTTCAAATGTATATTTTGAATTTACaaaa             >  1:723412/1‑251 (MQ=255)                                                                                                                                                                                  atatTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTc                                                                                              <  1:245615/159‑1 (MQ=255)                                                                                                                                                                                  atatTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTCATTAAACCCATGAAGGAAACATAGAAAACCATTCgt                                                          <  1:138133/195‑1 (MQ=255)                                                                                                                                                                                  atatTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTCATTAAACCCATGAAGGAAACATAGAAAACCATTCGTGTAATATTAATTTCTTTTTCAAATGTATATTTTGAATTTACAAAAAAAGAGAatat  >  1:593721/1‑251 (MQ=255)                                                                                                                                                                                         ccTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAAt                                                                                                             <  1:571449/137‑1 (MQ=255)                                                                                                                                                                                                                     attTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATgaga                                                                                                    >  1:266138/1‑118 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                             TTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCACTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAATAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTCATTAAACCCATGAAGGAAACATAGAAAACCATTCGTGTAATATTAATTTCTTTTTCAAATGTATATTTTGAATTTACAAAAAAAGAGAATAT  >  1/1076905‑1077332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑