Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,076,955	A→G	S43S (AGT→AGC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,076,955	0	A	G	100.0%	119.2 / NA	42	S43S (AGT→AGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/25);  total (17/25)

ATAAAAAAATTAATAGATAAAGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCACTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAATAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAA  >  1/1076717‑1077174                                                                                                                                                                                                                                               |                                                                                                                                                                                                                            ataaAAAAATTAATAGATAAAGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGaaa                                                                                                                                                                                                                 >  1:308377/1‑251 (MQ=255)                 aTAAAGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTc                                                                                                                                                                                                  <  1:659142/250‑1 (MQ=255)                    aaGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGt                                                                                                                                                                                              >  1:431868/1‑251 (MQ=255)                       gAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTc                                                                                                                                                                                            <  1:376701/250‑1 (MQ=255)                            gtgtTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTc                                                                                                                                                                                            <  1:770010/245‑1 (MQ=255)                            gtgtTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGtata                                                                                                                                                                                      >  1:690910/1‑251 (MQ=255)                                               ggTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTc                                                                                                                                                                                            >  1:446711/1‑226 (MQ=255)                                                gTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACataa                                                                                                                                                                  >  1:220898/1‑251 (MQ=255)                                                    aTGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACataat                                                                                                                                                                 >  1:238992/1‑248 (MQ=255)                                                     tGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATcc                                                                                                                                                             <  1:335323/251‑1 (MQ=255)                                                          tgtTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAaat                                                                                                                                                         <  1:806832/250‑1 (MQ=255)                                                           gtTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTc                                                                                                                                                                                            >  1:118337/1‑214 (MQ=255)                                                                 aTCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAACACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAg                                                                                                                                                 <  1:566724/251‑1 (MQ=255)                                                                   cGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCg                                                                                                                                               <  1:312250/251‑1 (MQ=255)                                                                            tAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACt                                                                                                                                      <  1:851922/251‑1 (MQ=255)                                                                             aGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATc                                                                                                                                                              >  1:209570/1‑226 (MQ=255)                                                                                  caagcaGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAAtt                                                                                                                                  <  1:580298/249‑1 (MQ=255)                                                                                        gggCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTc                                                                                                                                                                                            <  1:548977/185‑1 (MQ=255)                                                                                                              atCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGAc                                                                                                                                                                                                         <  1:671963/150‑1 (MQ=255)                                                                                                                     aCTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTc                                                                                                                                                                                            <  1:617823/156‑1 (MQ=255)                                                                                                                     aCTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCg                                                                                                                                               <  1:245950/201‑1 (MQ=255)                                                                                                                     aCTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGata                                                                                                                                            <  1:254015/204‑1 (MQ=255)                                                                                                                     aCTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACt                                                                                                                                      <  1:519896/210‑1 (MQ=255)                                                                                                                      ctctGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAg                                                                                                                                                 <  1:551128/198‑1 (MQ=255)                                                                                                                          ggACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTc                                                                                                                                                                                                                          >  1:668818/1‑121 (MQ=255)                                                                                                                            aCCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAAc                                                                                       <  1:81424/250‑1 (MQ=255)                                                                                                                                ttGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACat                                                                                             >  1:538508/2‑240 (MQ=255)                                                                                                                                gTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTc                                                                                                                                                                                                                          >  1:122548/1‑115 (MQ=255)                                                                                                                                gTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACt                                                                                                                                      <  1:411281/199‑1 (MQ=255)                                                                                                                                gTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCtttt                                                                                  >  1:252364/1‑251 (MQ=255)                                                                                                                                     tGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATAc                                                                                                                                                    >  1:113103/1‑180 (MQ=255)                                                                                                                                     tGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCg                                                                                                                                               <  1:284168/185‑1 (MQ=255)                                                                                                                                                       ggTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACt                                                                                                                                      <  1:898274/176‑1 (MQ=255)                                                                                                                                                       ggTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACttatt                                                            <  1:291422/250‑1 (MQ=255)                                                                                                                                                                   gATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGaaaaac                                                <  1:480259/250‑2 (MQ=255)                                                                                                                                                                            gTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTc                                                                                                                                                                                                                          <  1:37110/71‑1 (MQ=255)                                                                                                                                                                                             ttttttAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATaa                              <  1:560144/242‑1 (MQ=255)                                                                                                                                                                                                              atatTGATGATAGCGCATAAGATCATCAGTATCGCTc                                                                                                                                                                                                                          >  1:452168/1‑37 (MQ=255)                                                                                                                                                                                                                atTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGt                                                                                                                         >  1:801285/1‑132 (MQ=255)                                                                                                                                                                                                                atTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGaa  >  1:842868/1‑251 (MQ=255)                                                                                                                                                                                                                     tgatAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAAc                                                                                                                                       >  1:344144/1‑113 (MQ=255)                                                                                                                                                                                                                                  gATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATAc                                                                                                                                                    <  1:31549/87‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                            ATAAAAAAATTAATAGATAAAGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAACTCTGGACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCACTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAATAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAA  >  1/1076717‑1077174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑