Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,076,588	G→A	L166F (CTT→TTT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,076,588	0	G	A	100.0%	56.2 / NA	21	L166F (CTT→TTT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/8);  total (13/8)

AGATAGAATACTACTATGCAGGCATATTTTTTTGGCTAATTCTAAGTAATATAAATTATTCAGTAAACCATATAAAACAAATAGCATTAAAATGGCAACAAAGCTATATATGTTTTCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAGCTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGTGATATAATTAATAAAAAAATTAATAGATAAAGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAAC  >  1/1076358‑1076834                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aGATAGAATACTACTATGCAGGCATATTTTTTTGGCTAATTCTAAGTAATATAAATTATTCAGTAAACCATATAAAACAAATAGCATTAAAATGGCAACAAAGCTATATATGTTTTCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTa                                                                                                                                                                                                                                    >  1:485561/1‑251 (MQ=255)                                            aaGTAATATAAATTATTCAGTAAACCATATAAAACAAATAGCATTAAAATGGCAACAAAGCTATATATGTTTTCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAg                                                                                                                                                                                            >  1:93246/1‑248 (MQ=255)                                                   ataAATTATTCAGTAAACCATATAAAACAAATAGCATTAAAATGGCAACAAAGCTATATATGTTTTCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAg                                                                                                                                                                                   >  1:500089/1‑250 (MQ=255)                                                     aaaTTATTCAGTAAACCATATAAAACAAATAGCATTAAAATGGCAACAAAGCTATATATGTTTTCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCAt                                                                                                                                                                                >  1:74465/1‑251 (MQ=255)                                                                tAAACCATATAAAACAAATAGCATTAAAATGGCAACAAAGCTATATATGTTTTCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTAttt                                                                                                                                                                        <  1:360904/248‑1 (MQ=255)                                                                   aCCATATAAAACAAATAGCATTAAAATGGCAACAAAGCTATATATGTTTTCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAg                                                                                                                                                                                            <  1:729997/225‑1 (MQ=255)                                                                                                                   ttCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAAc                                                                                                                                                                                                                  >  1:157949/1‑155 (MQ=255)                                                                                                                        gCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGt                                                                                                                                  <  1:103793/230‑1 (MQ=255)                                                                                                                             aTCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAg                                                                                                                                                                                                                                 <  1:730983/130‑1 (MQ=255)                                                                                                                             aTCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGAAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGt                                                                                                                                  >  1:453886/1‑225 (MQ=255)                                                                                                                                     tGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAAt                                                                                                                                                                                              <  1:476619/157‑1 (MQ=255)                                                                                                                                     tGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAg                                                                                                                                                                                            >  1:571738/1‑159 (MQ=255)                                                                                                                                     tGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAAc                                                                                                                                                                                     >  1:636464/1‑166 (MQ=255)                                                                                                                                     tGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGt                                                                                                                                  >  1:140143/1‑217 (MQ=255)                                                                                                                                              tataCTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGGAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGTGATATAATTAATAAAAAAATTAATAGATAAAGGAGTGGTGTta                                                                                       <  1:166331/251‑1 (MQ=255)                                                                                                                                                         cTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAAc                                                                                                                                                >  1:893759/1‑183 (MQ=255)                                                                                                                                                                             ttttGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGTGATATAATTAATAAAAAAAt                                                                                                              >  1:886481/1‑197 (MQ=255)                                                                                                                                                                                                              aTGCAAAAAGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGccc                                                                                                                                                        <  1:323370/122‑1 (MQ=255)                                                                                                                                                                                                                   aaaaGATATTAATAAAATAAACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGTGATATAATTAATAAAAAAATTAATAGATAAAGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAggg                              >  1:134349/1‑239 (MQ=255)                                                                                                                                                                                                                                aaaataaACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGTGATATAATTAATAAAAAAATTAATAGATAAAgg                                                                                                 >  1:450688/1‑159 (MQ=255)                                                                                                                                                                                                                                   ataaACTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGTGATATAATTAATAAAAAAATTAATAGATAAAGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGAGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTCCCCCGGCTAATGGAATATCCACAAc  <  1:442530/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AGATAGAATACTACTATGCAGGCATATTTTTTTGGCTAATTCTAAGTAATATAAATTATTCAGTAAACCATATAAAACAAATAGCATTAAAATGGCAACAAAGCTATATATGTTTTCTAGCCCTATCCATAATGATAAAACTATACTTATCGCTATAAAGAATGGCTGCCCGTTTTGATTTTGTTTATTTTTTGCTATAGAGAAAATGCAAAAAGATATTAATAAAATAAGCTGAAGATTATGGTGATCTAAAGCGCCCGGAAGAAAACGATAACTAATTGGTGCTAATAGTGGAAACAGCATTGCTATTTTAGCTGTCTCCAGCCCAAATAAACGGTAAGATATTAGTGATATAATTAATAAAAAAATTAATAGATAAAGGAGTGGTGTTATAACATTCGATATGGTGAATGCGGTGTTCCAATCGGTGAAAATAGAAGCAAGCAGGGCTACCCCGGCTAATGGAATATCCACAAC  >  1/1076358‑1076834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑