Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,075,673	T→C	K471E (AAA→GAA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,075,673	0	T	C	100.0%	33.0 / NA	13	K471E (AAA→GAA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/5);  total (8/5)

ATAATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTTCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGC  >  1/1075464‑1075880                                                                                                                                                                                                                  |                                                                                                                                                                                                                ataATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAAtct                                                                                                                                                                        <  1:527916/251‑1 (MQ=255)                                            gTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGt                                                                                                                             >  1:197881/1‑251 (MQ=255)                                            gTATTAACTGACGTATAAAACCGCATT‑TATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATg                                                                                                                                        <  1:336391/239‑1 (MQ=255)                                                      aCGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAAt                                                                                                                   <  1:336669/251‑1 (MQ=255)                                                                       gTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGa                                                                                                  >  1:438127/1‑251 (MQ=255)                                                                             gAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGc                                                                                                                                                                                                   >  1:772459/1‑148 (MQ=255)                                                                                    ctctTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTAt                                                                                                                                         <  1:446569/199‑1 (MQ=255)                                                                                      ctTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGAt                                                                                   >  1:16505/1‑251 (MQ=255)                                                                                                 gtgGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTg                                                                                                                           >  1:147249/1‑200 (MQ=255)                                                                                                                            ggATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATg                                             >  1:414799/1‑251 (MQ=255)                                                                                                                                      gCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATg                                                                                                            >  1:487752/1‑178 (MQ=255)                                                                                                                                                                     gTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCAt                                                                                                                                                      >  1:5463/1‑105 (MQ=255)                                                                                                                                                                       aaTATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGc  <  1:459835/251‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                ATAATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTTCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGC  >  1/1075464‑1075880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑