Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,072,100	C→T	E353K (GAA→AAA)	unknown ←	DNA mismatch repair protein MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,072,100	0	C	T	100.0%	212.2 / NA	74	E353K (GAA→AAA)	unknown	DNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/40);  total (34/40)

CTATGGTCATTTCCGTTTGAGGCTGATTTTTTAACGCCGTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTCCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAA  >  1/1071854‑1072323                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                cTATGGTCATTTCCGTTTGAGGCTGATTTTTTAACGCAGTCAACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCcac                                                                                                                                                                                                                             <  1:165885/251‑1 (MQ=255)   aTGGTCATTTCCGTTTGAGGCTGATTTTTTAACGCCGTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCcacac                                                                                                                                                                                                                           <  1:203788/251‑1 (MQ=255)          tttCCGTTTGAGGCTGATTTTTTAACGCCGTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCa                                                                                                                                                                                                                    >  1:558768/1‑251 (MQ=255)               gTTTGAGGCTGATTTTTTAACGCCGTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGtt                                                                                                                                                                                                                >  1:791050/1‑250 (MQ=255)                    aGGCTGATTTTTTAACGCCGTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTtcttct                                                                                                                                                                                                           <  1:750536/250‑1 (MQ=255)                       cTGATTTTTTAACGCCGTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACgg                                                                                                                                                                                                       <  1:176641/251‑1 (MQ=255)                                       gTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGTATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGt                                                                                                                                                                                                                 <  1:451241/225‑1 (MQ=255)                                       gTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCAt                                                                                                                                                                                                    <  1:621147/238‑1 (MQ=255)                                       gTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATtgatga                                                                                                                                                                                       >  1:597620/1‑251 (MQ=255)                                         ccACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACg                                                                                                                                                                                     >  1:508334/1‑251 (MQ=255)                                              aGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGt                                                                                                                                                                                                                 <  1:505716/218‑1 (MQ=255)                                                gTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCaa                                                                                                                                                                              >  1:877920/1‑251 (MQ=255)                                                gTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCaa                                                                                                                                                                              >  1:341240/1‑251 (MQ=255)                                                    tgtgGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGGGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAgtgt                                                                                                                                                                          <  1:877458/251‑1 (MQ=255)                                                        ggCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTAc                                                                                                                                                                                                         <  1:263533/216‑1 (MQ=255)                                                         gCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTtcttct                                                                                                                                                                                                           <  1:606923/213‑1 (MQ=255)                                                          cATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTg                                                                                                                                                                     <  1:165876/250‑1 (MQ=255)                                                          cATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTg                                                                                                                                                                     <  1:878762/250‑1 (MQ=255)                                                           aTTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGt                                                                                                                                                                                                                 <  1:54410/205‑1 (MQ=255)                                                            ttCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGtt                                                                                                                                                                   >  1:665224/1‑250 (MQ=255)                                                            ttCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTc                                                                                                                                                                  <  1:276347/251‑1 (MQ=255)                                                             tCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGtt                                                                                                                                                                   >  1:588778/1‑249 (MQ=255)                                                              cAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGtt                                                                                                                                                                   >  1:331288/1‑248 (MQ=255)                                                                 tACGTTCTGCTTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCTTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAg                                                                                                                                                             <  1:386709/251‑1 (MQ=255)                                                                 tACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAg                                                                                                                                                             <  1:506396/251‑1 (MQ=255)                                                                                  gTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATtga                                                                                                                                                                                          >  1:487686/1‑205 (MQ=255)                                                                                  gTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATg                                                                                                                                            >  1:835946/1‑251 (MQ=255)                                                                                   ttCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATg                                                                                                                                            >  1:271761/1‑250 (MQ=255)                                                                                    tCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGcc                                                                                                                                          <  1:242798/251‑1 (MQ=255)                                                                                    tCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGcc                                                                                                                                          <  1:895331/251‑1 (MQ=255)                                                                                       cGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTg                                                                                                                                       >  1:205831/1‑251 (MQ=255)                                                                                         aTGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTggg                                                                                                                                     <  1:6016/251‑1 (MQ=255)                                                                                                ttttCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGaa                                                                                                                              >  1:263829/1‑251 (MQ=255)                                                                                                 tttCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGaaa                                                                                                                             >  1:253339/1‑251 (MQ=255)                                                                                                        aTAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCCGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCa                                                                                                                      >  1:817280/1‑251 (MQ=255)                                                                                                         tAAAAGTGCGGTGATCTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCAc                                                                                                                     <  1:53964/251‑1 (MQ=255)                                                                                                         tAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCAc                                                                                                                     <  1:160253/251‑1 (MQ=255)                                                                                                                   gTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCAc                                                                                                                     >  1:348858/1‑241 (MQ=255)                                                                                                                       aCTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTg                                                                                                         >  1:237122/1‑249 (MQ=255)                                                                                                                       aCTTTGCCGGTG‑TTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTg                                                                                                      <  1:898557/251‑1 (MQ=255)                                                                                                                          ttGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACgggg                                                                                                                                                                                  >  1:307078/1‑173 (MQ=255)                                                                                                                                gtgtTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAgtgt                                                                                                                                                                          <  1:880591/175‑1 (MQ=255)                                                                                                                                gtgtTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCa                                                                                              >  1:266056/1‑251 (MQ=255)                                                                                                                                  gtTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAAc                                                                                                 <  1:174294/246‑1 (MQ=255)                                                                                                                                    ttttGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTc                                                                                                                                                                  <  1:118479/179‑1 (MQ=255)                                                                                                                                             ccGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGt                                                                                 >  1:752802/1‑251 (MQ=255)                                                                                                                                                    tCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGc                                                                          <  1:278317/251‑1 (MQ=255)                                                                                                                                                          tttCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTc                                                                    <  1:646442/251‑1 (MQ=255)                                                                                                                                                              ggCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACAt                                                                >  1:574963/1‑251 (MQ=255)                                                                                                                                                               gCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGcac                                                                        >  1:138499/1‑242 (MQ=255)                                                                                                                                                               gCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACAt                                                                <  1:129597/250‑1 (MQ=255)                                                                                                                                                                cGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                              <  1:687346/251‑1 (MQ=255)                                                                                                                                                                cGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                              <  1:88721/251‑1 (MQ=255)                                                                                                                                                                         tGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTc                                                     <  1:325928/251‑1 (MQ=255)                                                                                                                                                                              gAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                              >  1:442787/1‑237 (MQ=255)                                                                                                                                                                               aaTAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTa                                                                                                                                                                                                          >  1:657932/1‑96 (MQ=255)                                                                                                                                                                                    ccGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCgtg                                                                                       <  1:124547/206‑1 (MQ=255)                                                                                                                                                                                    ccGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGAt                                           >  1:811024/1‑250 (MQ=255)                                                                                                                                                                                           tCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATaa                                     <  1:168800/249‑1 (MQ=255)                                                                                                                                                                                              gTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGcc                                                                                                                                                           <  1:752454/128‑1 (MQ=255)                                                                                                                                                                                              gTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAaacaa                                 >  1:799222/1‑250 (MQ=255)                                                                                                                                                                                                 cGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATaa                                     <  1:597342/243‑1 (MQ=255)                                                                                                                                                                                                   aaTATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTTTTCTACGGCATTTTCCATTGATGACGTGGTCAAGTGTATTTGCTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACaaa                           >  1:607227/1‑251 (MQ=255)                                                                                                                                                                                                   aaTATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTggg                                                                                                                                     >  1:148545/1‑145 (MQ=255)                                                                                                                                                                                                   aaTATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACaaa                           >  1:408079/1‑251 (MQ=255)                                                                                                                                                                                                        atTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCgtg                                                                                       >  1:452526/1‑186 (MQ=255)                                                                                                                                                                                                        atTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                              >  1:679809/1‑211 (MQ=255)                                                                                                                                                                                                              cccTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGatat                 <  1:132206/250‑1 (MQ=255)                                                                                                                                                                                                                  gccgccGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCg                                                                                                               <  1:184251/152‑1 (MQ=255)                                                                                                                                                                                                                   ccgccgAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCa           >  1:482566/1‑251 (MQ=255)                                                                                                                                                                                                                             aTTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGatat                 >  1:522129/1‑235 (MQ=255)                                                                                                                                                                                                                             aTTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTaaa  <  1:809214/250‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAaac                                   <  1:315398/212‑1 (MQ=255)                                                                                                                                                                                                                                      ggTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGtgatga                                                                                                                               <  1:652365/116‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                CTATGGTCATTTCCGTTTGAGGCTGATTTTTTAACGCCGTCCACAAGGTTTTGTGGGCATTCAATACGTTCTGCGTAATTTGTTCACGATGGGCATTTTCACGATAAAAGTGCGGTGAACTTTGCCGGTGTTTTTGCGTGCCGTCATTCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTCCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAA  >  1/1071854‑1072323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑