Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,070,907	A→G	S129P (TCA→CCA)	unknown ←	tRNA dimethylallyltransferase (EC 2.5.1.75)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,070,907	0	A	G	100.0%	194.6 / NA	66	S129P (TCA→CCA)	unknown	tRNA dimethylallyltransferase (EC 2.5.1.75)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/26);  total (40/26)

AACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGACCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACTCGGCTTTTGAGGGCTTTGCCGTGCCAATATCC  >  1/1070669‑1071148                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  aaCAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTc                                                                                                                                                                                                                                       <  1:323557/251‑1 (MQ=255)     gATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATcg                                                                                                                                                                                                                                   >  1:145214/1‑251 (MQ=255)     gATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTCTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATcg                                                                                                                                                                                                                                   >  1:895675/1‑251 (MQ=255)                 ccGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAAt                                                                                                                                                                                                                       >  1:857563/1‑251 (MQ=255)                    gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                                                                                                                                                                                                                     >  1:538148/1‑250 (MQ=255)                    gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                                                                                                                                                                                                                     <  1:877740/250‑1 (MQ=255)                    gTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATgg                                                                                                                                                                                                                     >  1:195680/1‑250 (MQ=255)                            gCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGc                                                                                                                                                                                                             <  1:348426/250‑1 (MQ=255)                             cGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGcc                                                                                                                                                                                                            <  1:332588/250‑1 (MQ=255)                                 ttGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGa                                                                                                                                                                                                       >  1:642812/1‑251 (MQ=255)                                        aTCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACg                                                                                                                                                                                                 >  1:431110/1‑250 (MQ=255)                                        aTCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACg                                                                                                                                                                                                 >  1:641772/1‑250 (MQ=255)                                        aTCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACg                                                                                                                                                                                                 >  1:896894/1‑250 (MQ=255)                                         tctcATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACg                                                                                                                                                                                                 >  1:834902/1‑249 (MQ=255)                                          ctcATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCtt                                                                                                                                                                                              >  1:755139/1‑251 (MQ=255)                                           tcATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCttt                                                                                                                                                                                             >  1:167989/1‑251 (MQ=255)                                           tcATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCttt                                                                                                                                                                                             >  1:362351/1‑251 (MQ=255)                                           tcATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCttt                                                                                                                                                                                             >  1:343767/1‑251 (MQ=255)                                           tcATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGCTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCttt                                                                                                                                                                                             >  1:725514/1‑251 (MQ=255)                                                      cgcgTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCTTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGcc                                                                                                                                                                                                            <  1:770822/225‑1 (MQ=255)                                                      cgcgTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATacaac                                                                                                                                                                                   <  1:605553/250‑1 (MQ=255)                                                        cgTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACAt                                                                                                                                                                                 <  1:522426/250‑1 (MQ=255)                                                          tCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTc                                                                                                                                                                                                                                       >  1:182724/1‑194 (MQ=255)                                                                         gTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGc                                                                                                                                                                                                     <  1:326923/213‑1 (MQ=255)                                                                             aTTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACggg                                                                                                                                                           >  1:320891/1‑251 (MQ=255)                                                                                ccGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTaa                                                                                                                                                                                         <  1:808698/218‑1 (MQ=255)                                                                                ccGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGAtt                                                                                                                                                        >  1:26905/1‑251 (MQ=255)                                                                                     aaGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTAcc                                                                                                                                                   <  1:615286/251‑1 (MQ=255)                                                                                        gTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTAccc                                                                                                                                                  <  1:104056/249‑1 (MQ=255)                                                                                          tttGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACg                                                                                                                                              <  1:99337/251‑1 (MQ=255)                                                                                                    aaataaaACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACAt                                                                                                                                                                                 >  1:5962/1‑206 (MQ=255)                                                                                                    aaataaaACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGt                                                                                                                                     >  1:39292/1‑250 (MQ=255)                                                                                                    aaataaaACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGt                                                                                                                                     >  1:409311/1‑250 (MQ=255)                                                                                                      ataaaACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGt                                                                                                                                                                                           <  1:386253/194‑1 (MQ=255)                                                                                                      ataaaACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTAccc                                                                                                                                                  <  1:646127/235‑1 (MQ=255)                                                                                                          aaCACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCAt                                                                                                                              <  1:802034/251‑1 (MQ=255)                                                                                                          aaCACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCAt                                                                                                                              <  1:461280/251‑1 (MQ=255)                                                                                                            cacTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGc                                                                                                                                                                                                     >  1:336735/1‑178 (MQ=255)                                                                                                            cacTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCAttt                                                                                                                            >  1:606457/1‑251 (MQ=255)                                                                                                              cTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCa                                                                                                                          >  1:746981/1‑251 (MQ=255)                                                                                                                    gTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAgg                                                                                                                    >  1:57593/1‑251 (MQ=255)                                                                                                                                                 gggTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAggg                                                                                                                   >  1:424813/1‑223 (MQ=255)                                                                                                                                                 gggTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCgg                                                                                                             <  1:710662/229‑1 (MQ=255)                                                                                                                                                   gTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAg                                                                                      >  1:322183/1‑250 (MQ=255)                                                                                                                                                                               aTTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTaa                                                         >  1:606378/1‑251 (MQ=255)                                                                                                                                                                                 tttGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAAc                                                       <  1:423836/251‑1 (MQ=255)                                                                                                                                                                                   tGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAAtt                                                           <  1:340657/245‑1 (MQ=255)                                                                                                                                                                                   tGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACgg                                                     <  1:201946/251‑1 (MQ=255)                                                                                                                                                                                         gTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGaaa                                                                                                     >  1:293875/1‑197 (MQ=255)                                                                                                                                                                                         gTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTgggg                                                >  1:424431/1‑250 (MQ=255)                                                                                                                                                                                         gTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTgggg                                                >  1:624632/1‑250 (MQ=255)                                                                                                                                                                                            ccTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGcc                                            <  1:335190/251‑1 (MQ=255)                                                                                                                                                                                                aTGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATacaac                                                                                                                                                                                   >  1:427043/1‑112 (MQ=255)                                                                                                                                                                                                aTGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATg                                                                                                                                      >  1:878537/1‑157 (MQ=255)                                                                                                                                                                                                aTGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGt                                                                                                                                     >  1:510977/1‑158 (MQ=255)                                                                                                                                                                                                aTGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTggc                                                                                                >  1:720819/1‑195 (MQ=255)                                                                                                                                                                                                       gccgccCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACt                                  >  1:790757/1‑250 (MQ=255)                                                                                                                                                                                                               gCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGcc                                            >  1:393087/1‑232 (MQ=255)                                                                                                                                                                                                               gCCGATACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACTCGGCTTTTg                         >  1:522278/1‑251 (MQ=255)                                                                                                                                                                                                                    tACGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACTCGGCTTTTGAGGGc                    <  1:358575/251‑1 (MQ=255)                                                                                                                                                                                                                      cGTTCCGCTTTCGCTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCcacccacc                                                                                                                                                                    >  1:150796/1‑105 (MQ=255)                                                                                                                                                                                                                                   cTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAGCGGGATTTTACCCAACGAGGTAATGTCCTGCAttt                                                                                                                            <  1:566439/132‑1 (MQ=255)                                                                                                                                                                                                                                   cTTCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACTCGGCTTTTGAGGGCTTTGCCGTGCCAata     >  1:131418/1‑251 (MQ=255)                                                                                                                                                                                                                                     tCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACTCGGCTTTTGAGGGCTTTGCCGTGCCAATATc   <  1:26663/251‑1 (MQ=255)                                                                                                                                                                                                                                     tCAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACTCGGCTTTTGAGGGCTTTGCCGTGCCAATATc   <  1:322916/251‑1 (MQ=255)                                                                                                                                                                                                                                      cAATTTCTGGCCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACTCGGCTTTTGAGGGCTTTGCCGTGCCAATATcc  <  1:731971/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  AACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGCAGCGCGTCGCCTTTTTGTTCGGTTAATTCCGTTAAGGTTTTGCCGCTTAAATAAAACACTTCCAGTGCGCGGTTAATCCGTTGCGAATCATTCGGGTTAATGCGTTGCGCCGAGATGGGGTCGATTTTGTTCAGTTCCTGATGTAATGCCGCCCAGCCGATACGTTCCGCTTTCGCTTCAATTTCTGACCGCACTTTTTCATCGGCGGAGGGCAATGGCGATAAGCCTTCGAGCAACGCTTTGTAATACAACATCGTGCCACCCACCAATAACGGGATTTTACCCAACGAGGTAATGTCCTGCATTTCACGTAGGGCGTCGGTGCGGAAATTGGCGGCGGAATAGCTTTCTGCCGGGTCGCAAATATCAATTAAACGGTGGGGCGCCAGTGCCAACTCGGCTTTTGAGGGCTTTGCCGTGCCAATATCC  >  1/1070669‑1071148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑