Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,069,941	T→A	Q96L (CAG→CTG)	unknown ←	RNA‑binding protein Hfq

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,069,941	0	T	A	100.0%	143.7 / NA	51	Q96L (CAG→CTG)	unknown	RNA‑binding protein Hfq
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/33);  total (18/33)

AAAATATTGTCTTGAGCGGATTTCGTGAGCAGTTGAAATTCATTTAAATCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCTGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGCCCCTGTAATTTGATACCGTTTACC  >  1/1069698‑1070150                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                  aaaaTATTGTCTTGAGCGGATTTCGTGAGCAGTTGAAATTCATTTAAATCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCtt                                                                                                                                                                                                             >  1:475803/1‑250 (MQ=255)    atatTGTCTTGAGCGGATTTCGTGAGCAGTTGAAATTCATTTAAATCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAAc                                                                                                                                                                                                         <  1:319077/251‑1 (MQ=255)         gTCTTGAGCGGATTTCGTGAGCAGTTGAAATTCATTTAAATCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGt                                                                                                                                                                                                    >  1:204798/1‑251 (MQ=255)                            aGCAGTTGAAATTCATTTAAATCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGttt                                                                                                                                                                                  <  1:401504/250‑1 (MQ=255)                              cAGTTGAAATTCATTTAAATCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCtt                                                                                                                                                                               >  1:375979/1‑251 (MQ=255)                                            ttAAATCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGa                                                                                                                                                                 <  1:128792/251‑1 (MQ=255)                                              aaaTCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATg                                                                                                                                                               >  1:601041/1‑251 (MQ=255)                                                 tcatcCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCTCTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTtg                                                                                                                                                            <  1:52452/251‑1 (MQ=255)                                                       gTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTtgctg                                                                                                                                                         >  1:601855/1‑248 (MQ=255)                                                       gTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTtgctg                                                                                                                                                         >  1:781674/1‑248 (MQ=255)                                                         aTTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGAt                                                                                                                                                                <  1:549576/239‑1 (MQ=255)                                                                  tgAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCAt                                                                                                                                           <  1:310064/251‑1 (MQ=255)                                                                    aaTCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATtg                                                                                                                                         <  1:217507/251‑1 (MQ=255)                                                                       cAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGttaaccaacagcagcaacatcaggg                                                                                                                                      <  1:74760/251‑24 (MQ=255)                                                                                 aTGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGttt                                                                                                                                                                                  <  1:852710/197‑1 (MQ=255)                                                                                 aTGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGctgctg                                                                                                                                                      >  1:77250/1‑225 (MQ=255)                                                                                      cGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATg                                                                                                                                                               >  1:8712/1‑211 (MQ=255)                                                                                        aTAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGtt                                                                                                                                                <  1:74192/224‑1 (MQ=255)                                                                                        aTAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAAcgcg                                                                                                                      >  1:761415/1‑250 (MQ=255)                                                                                        aTAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAAcgcg                                                                                                                      >  1:103718/1‑250 (MQ=255)                                                                                              gCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGcc                                                                                                                    >  1:707623/1‑246 (MQ=255)                                                                                                 tGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTacaac                                                                                                            <  1:140408/251‑1 (MQ=255)                                                                                                   aTTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGagt                                                                                                                                                                                       <  1:372553/174‑1 (MQ=255)                                                                                                        aTCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCgag                                                                                                     <  1:604719/251‑1 (MQ=255)                                                                                                               aTTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAg                                                                                                          <  1:665595/239‑1 (MQ=255)                                                                                                                           atgaGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCa                                                                                  <  1:582595/251‑1 (MQ=255)                                                                                                                               gggATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAAc                                                                                    <  1:56935/245‑1 (MQ=255)                                                                                                                                  aTTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGtt                                                                                                                                                <  1:800535/182‑1 (MQ=255)                                                                                                                                  aTTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGAt                                                                            <  1:311997/250‑1 (MQ=255)                                                                                                                                   ttATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGAttt                                                                          >  1:330467/1‑251 (MQ=255)                                                                                                                                               tCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTc                                                              <  1:563017/251‑1 (MQ=255)                                                                                                                                                  gACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGt                                                           >  1:139217/1‑251 (MQ=255)                                                                                                                                                                 gTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGCTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGa                                            >  1:1930/1‑251 (MQ=255)                                                                                                                                                                  tGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGAt                                           <  1:795691/251‑1 (MQ=255)                                                                                                                                                                   ggattctgcggaaatttCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGAt                                                                                                   <  1:257746/178‑1 (MQ=255)                                                                                                                                                                      aaTTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGaa                                       >  1:655830/1‑251 (MQ=255)                                                                                                                                                                        tttCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATg                                     <  1:493368/251‑1 (MQ=255)                                                                                                                                                                                    ccACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAAc                                                                                    >  1:590590/1‑192 (MQ=255)                                                                                                                                                                                    ccACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTc                                                              <  1:804321/214‑1 (MQ=255)                                                                                                                                                                                     cACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGcc                         >  1:211557/1‑250 (MQ=255)                                                                                                                                                                                      aCCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGcccc                       <  1:18169/251‑1 (MQ=255)                                                                                                                                                                                      aCCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGcccc                       <  1:343690/251‑1 (MQ=255)                                                                                                                                                                                             tttGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGCCCCTGTAAtt                <  1:377364/251‑1 (MQ=255)                                                                                                                                                                                              ttGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGCCCCTGTAAttt               <  1:581972/251‑1 (MQ=255)                                                                                                                                                                                              ttGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGCCCCTGTAAttt               <  1:486372/251‑1 (MQ=255)                                                                                                                                                                                                ggCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGCCCCTGTAATTTGa             >  1:693779/1‑251 (MQ=255)                                                                                                                                                                                                     aaaTTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAAc                                                                                    <  1:314619/175‑1 (MQ=255)                                                                                                                                                                                                           ttGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGCCCCTGTAATTTGATACCGTTTAcc  <  1:619742/251‑1 (MQ=255)                                                                                                                                                                                                             gAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGAtt                             <  1:318507/222‑1 (MQ=255)                                                                                                                                                                                                              aGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACtg                                                                      <  1:322424/180‑1 (MQ=255)                                                                                                                                                                                                                                       tCGTTATTCTGCCAGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAAcc                                                                                   <  1:640132/142‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                  AAAATATTGTCTTGAGCGGATTTCGTGAGCAGTTGAAATTCATTTAAATCATCCGTATTTTTTTGTGAATCAAAAAGGCAATGCACGATAGTGGCATGATTCAATCCATGATTTTTCATGTGATGAGGGATTATCAGGCTTATCAGACGCAAAAAGTGCGGTGGAAATTTCCGCAGAATCCACCGCACTTTGGCTTAAATTATTGAGTAAAGGGTTCAATATTTTCAGCTTCGTTATTCTGCCTGAGCTTCAACATTGTTTTCGGCAGGAGTAGTTTCTTGCTGTTGTCCCTGATGTTGCTGCTGTTGGTTAGCATTGTGATGGGCGACGGAACGCGCCGGTACAACAGTCGAGATGGCGTGTTTATAAACCATTTGATTTACTGTGTTTTTCAGTAAAATCACGAATTGATCGAATGACTCGATTTGCCCCTGTAATTTGATACCGTTTACC  >  1/1069698‑1070150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑