Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,020,190	T→C	C16R (TGC→CGC)	unknown →	Ribonuclease G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,020,190	0	T	C	100.0%	242.6 / NA	83	C16R (TGC→CGC)	unknown	Ribonuclease G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/40);  total (43/40)

ATGCCGACGATATAAATAACAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTTGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGACATTTCC  >  1/1019954‑1020429                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                aTGCCGACGATATAAATAACAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTa                                                                                                                                                                                                                                   <  1:593569/251‑1 (MQ=255)         gaTATAAATAACAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAAc                                                                                                                                                                                                                              <  1:544668/248‑1 (MQ=255)              aaataaCAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTatat                                                                                                                                                                                                                      <  1:6119/251‑1 (MQ=255)                        tgtgATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACG‑AAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTa                                                                                                                                                                                                           >  1:350751/1‑251 (MQ=255)                          tgATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTa                                                                                                                                                                                                                    >  1:844757/1‑241 (MQ=255)                          tgATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTa                                                                                                                                                                                                           >  1:263969/1‑250 (MQ=255)                                ttgttgGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTg                                                                                                                                                                                                    >  1:730836/1‑251 (MQ=255)                                           aaTCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGc                                                                                                                                                                                         <  1:102899/251‑1 (MQ=255)                                           aaTCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGc                                                                                                                                                                                         <  1:817938/251‑1 (MQ=255)                                             tCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCaa                                                                                                                                                                                       >  1:349458/1‑251 (MQ=255)                                                 aaCATCGTACCAACCTTAGTACATAGTCTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAAcgc                                                                                                                                                                                   <  1:20686/251‑1 (MQ=255)                                                   cATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAAcgc                                                                                                                                                                                   >  1:36425/1‑249 (MQ=255)                                                      cGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTAt                                                                                                                                                                              <  1:473077/251‑1 (MQ=255)                                                       gTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTAtt                                                                                                                                                                             >  1:146436/1‑251 (MQ=255)                                                        tACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATtg                                                                                                                                                                            <  1:485169/251‑1 (MQ=255)                                                            aaCCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAg                                                                                                                                                                                                                                  <  1:663162/193‑1 (MQ=255)                                                             aCCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGa                                                                                                                                                                       <  1:861586/251‑1 (MQ=255)                                                              ccTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACatat                                                                                                                                                                                                      >  1:490227/1‑219 (MQ=255)                                                                ttAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTAc                                                                                                                                                                                                          <  1:488730/213‑1 (MQ=255)                                                                   gTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTg                                                                                                                                                                                                    >  1:521922/1‑216 (MQ=255)                                                                   gTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACAtt                                                                                                                                                                 >  1:244989/1‑251 (MQ=255)                                                                   gTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACAtt                                                                                                                                                                 >  1:646649/1‑251 (MQ=255)                                                                   gTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACAtt                                                                                                                                                                 >  1:299868/1‑251 (MQ=255)                                                                     aCATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGta                                                                                                                                                                                                                         >  1:242674/1‑193 (MQ=255)                                                                        tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGTCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:152439/251‑1 (MQ=255)                                                                        tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:806072/251‑1 (MQ=255)                                                                        tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:545726/251‑1 (MQ=255)                                                                        tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:391983/251‑1 (MQ=255)                                                                        tAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGACGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:73574/251‑1 (MQ=255)                                                                                       tgtgAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAaaga                                                                                                                                                                                                               <  1:415974/185‑1 (MQ=255)                                                                                       tgtgAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTAt                                                                                                                                                                              <  1:256217/218‑1 (MQ=255)                                                                                                               cATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAaag                                                                                                                                                                                                                <  1:307529/160‑1 (MQ=255)                                                                                                               cATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTg                                                                                                                                                                                                    >  1:75192/1‑172 (MQ=255)                                                                                                               cATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCgg                                                                                                                      >  1:5718/1‑250 (MQ=255)                                                                                                                  ttGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGtt                                                                                                                  <  1:579963/251‑1 (MQ=255)                                                                                                                        cTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGCCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGa                                                                                                            >  1:782035/1‑251 (MQ=255)                                                                                                                             attCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAg                                                                                                                                                                                                                                  >  1:463794/1‑128 (MQ=255)                                                                                                                             attCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTgg                                                                                                                                                                         >  1:95620/1‑185 (MQ=255)                                                                                                                             attCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            >  1:693846/1‑198 (MQ=255)                                                                                                                                cTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAaaga                                                                                                                                                                                                               <  1:861683/144‑1 (MQ=255)                                                                                                                                cTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAACTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCg                                                                                                                                   >  1:722538/1‑220 (MQ=255)                                                                                                                                     ttCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGaa                                                                                               <  1:216246/251‑1 (MQ=255)                                                                                                                                      tCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGaaa                                                                                              <  1:362457/251‑1 (MQ=255)                                                                                                                                            tCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:190086/183‑1 (MQ=255)                                                                                                                                            tCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAgccg                                                                                        >  1:59907/1‑251 (MQ=255)                                                                                                                                             cGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAgccg                                                                                        >  1:562417/1‑250 (MQ=255)                                                                                                                                                      tattaGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTatat                                                                                                                                                                                                                      >  1:163033/1‑115 (MQ=255)                                                                                                                                                      tattaGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCa                                                                              >  1:180814/1‑251 (MQ=255)                                                                                                                                                               aaaaaaaTTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:543371/164‑1 (MQ=255)                                                                                                                                                                 aaaaaTTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:475173/251‑1 (MQ=255)                                                                                                                                                                 aaaaaTTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:138932/251‑1 (MQ=255)                                                                                                                                                                 aaaaaTTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:616626/251‑1 (MQ=255)                                                                                                                                                                          cAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTcac                                                          <  1:532958/251‑1 (MQ=255)                                                                                                                                                                              cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTcacaca                                                       >  1:547642/1‑250 (MQ=255)                                                                                                                                                                              cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTcacaca                                                       >  1:869052/1‑250 (MQ=255)                                                                                                                                                                              cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTcacaca                                                       >  1:507883/1‑250 (MQ=255)                                                                                                                                                                              cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCacacac                                                      <  1:184539/251‑1 (MQ=255)                                                                                                                                                                              cTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCacacac                                                      <  1:264829/251‑1 (MQ=255)                                                                                                                                                                                           aCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAg                                                                                                                                                                                                                                  >  1:484991/1‑66 (MQ=255)                                                                                                                                                                                           aCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:526695/225‑1 (MQ=255)                                                                                                                                                                                           aCATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   >  1:65141/1‑225 (MQ=255)                                                                                                                                                                                            cATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATg                                        >  1:171309/1‑251 (MQ=255)                                                                                                                                                                                            cATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATg                                        <  1:427494/251‑1 (MQ=255)                                                                                                                                                                                              ttATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATc                                                                                                        >  1:62254/1‑185 (MQ=255)                                                                                                                                                                                              ttATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGaa                                      >  1:147221/1‑251 (MQ=255)                                                                                                                                                                                              ttATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCAGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGaa                                      >  1:438585/1‑251 (MQ=255)                                                                                                                                                                                              ttATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACACCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGaa                                      >  1:658522/1‑251 (MQ=255)                                                                                                                                                                                                           cGAATTATTGGTCAATGTGACACCCAACGAAGCTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGATCGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGc                         <  1:378647/251‑1 (MQ=255)                                                                                                                                                                                                             aaTTATTGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCaa                       >  1:148364/1‑251 (MQ=255)                                                                                                                                                                                                               ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTaa                                                                                                                                                                                                                   >  1:386450/1‑61 (MQ=255)                                                                                                                                                                                                               ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATtgt                                                                                                                                                                           <  1:876742/101‑1 (MQ=255)                                                                                                                                                                                                               ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGAcc                                                                                                                                               >  1:604258/1‑129 (MQ=255)                                                                                                                                                                                                               ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGc                                                                               <  1:868106/193‑1 (MQ=255)                                                                                                                                                                                                               ttattGGTCAATGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAAtt                     >  1:316926/1‑251 (MQ=255)                                                                                                                                                                                                                         aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACAt                                                                                                                                                                  <  1:818772/100‑1 (MQ=255)                                                                                                                                                                                                                         aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATaa                                                                                                                                                            <  1:281924/106‑1 (MQ=255)                                                                                                                                                                                                                         aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCtt                                                                                                   >  1:647047/1‑163 (MQ=255)                                                                                                                                                                                                                         aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACa                                                                   <  1:783639/195‑1 (MQ=255)                                                                                                                                                                                                                         aTGTGACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCCCACACTGAATGCGTGGATGAAAACGAACAAAAACAATTTGTGGTaaaa           >  1:723913/1‑251 (MQ=255)                                                                                                                                                                                                                           gtgACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGa         >  1:648598/1‑251 (MQ=255)                                                                                                                                                                                                                            tgACACCCAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGAc        <  1:710655/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCATACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGACATTTcc  >  1:712076/1‑250 (MQ=255)                                                                                                                                                                                                                                   cAACGAAACTCGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGACATTTcc  >  1:225962/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                ATGCCGACGATATAAATAACAAATGTGATAATTGTTGGATCTAATCCAAACATCGTACCAACCTTAGTACATAGACTGCTGAAAAATGTGAAAAGAGGCGCATTTTACCGCATTTGCCCCTATTATTCTATTTTCCTTTTCGTTAGACTTATTAGACGAAAAAAATTAGCAAACTTAAGAGCAGGGACATTATGGATTCGGTCGAATTATTGGTCAATGTGACACCCAACGAAACTTGCATTGCCCTTGTAGAAACAGGTATATTAAAAGAAGTACATATTGAACGCCAAGGCAAACGCGGTATTGTGGGAAACATTTATAAAGGGCGCGTGACCCGCGTGCTGCCGGGAATGCAGTCGGCGTTTGTGGACATCGGCTTGGAAAAAGCCGCCTTTTTGCACGCCTCAGACATCGTCTCACACACTGAATGCGTGGATGAAAACGAACAAAAGCAATTTGTGGTAAAAGACATTTCC  >  1/1019954‑1020429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑