Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 158,807 G→A intergenic (+441/‑48) unknown → / → unknown NADP‑specific glutamate dehydrogenase (EC 1.4.1.4)/Catalase KatE (EC 1.11.1.6)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1158,8070GA100.0% 98.8 / NA 36intergenic (+441/‑48)unknown/unknownNADP‑specific glutamate dehydrogenase (EC 1.4.1.4)/Catalase KatE (EC 1.11.1.6)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/16);  total (20/16)

TAAACAATTTGAGCCCTGTGCAGTGCAGAGCTCAATATTTAACTTAGGTTTAGCTATCCGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATGAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGCCAAGGGTTCCG  >  1/158575‑159053
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
tAAACAATTTGAGCCCTGTGCAGTGCAGAGCTCAATATTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTAc                                                                                                                                                                                                                                      <  1:575162/251‑1 (MQ=255)
            gCCCTGTGCAGTGCAGAGCTCAATATTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCaa                                                                                                                                                                                                                          >  1:110976/1‑251 (MQ=255)
                 gtgcagtgcaGAGCTCAATATTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAAtt                                                                                                                                                                                                                     >  1:287428/1‑251 (MQ=255)
                      gtgcagAGCTCAATATTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTc                                                                                                                                                                                                                            >  1:111448/1‑239 (MQ=255)
                                  atatTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGACAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGt                                                                                                                                                                                                     >  1:194101/1‑250 (MQ=255)
                                    atTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATAtttt                                                                                                                                                                                                                                          <  1:342551/211‑1 (MQ=255)
                                         aCTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAgg                                                                                                                                                                                                                 >  1:529649/1‑231 (MQ=255)
                                                                gTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAAc                                                                                                                                                                           <  1:867684/246‑1 (MQ=255)
                                                                       ggTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACAt                                                                                                                                                                 <  1:605270/249‑1 (MQ=255)
                                                                               tCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAActc                                                                                                                                                                         <  1:436063/233‑1 (MQ=255)
                                                                                                     tctcATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGAt                                                                                                                                 <  1:702290/251‑1 (MQ=255)
                                                                                                            tCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGaca                                                                                                                          <  1:81290/251‑1 (MQ=255)
                                                                                                                               cAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTg                                                                                                              <  1:352902/244‑1 (MQ=255)
                                                                                                                                        cTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATg                                                                                                                                                                                                      >  1:53398/1‑147 (MQ=255)
                                                                                                                                                  ttATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGtt                                                                                     <  1:750846/250‑1 (MQ=255)
                                                                                                                                                                      attattAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGa                                                                >  1:206480/1‑251 (MQ=255)
                                                                                                                                                                         attaATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAAc                                                                                                                                                                           <  1:439477/141‑1 (MQ=255)
                                                                                                                                                                           taatttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAAc                                                           <  1:422199/251‑1 (MQ=255)
                                                                                                                                                                            aatttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCCACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACt                                                          <  1:443021/251‑1 (MQ=255)
                                                                                                                                                                             atttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGac                                                                                                                           <  1:549078/185‑1 (MQ=255)
                                                                                                                                                                             atttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAAc                                                           >  1:618687/1‑249 (MQ=255)
                                                                                                                                                                             atttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTa                                                         >  1:341093/1‑251 (MQ=255)
                                                                                                                                                                             atttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCAGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTa                                                         >  1:450016/1‑251 (MQ=255)
                                                                                                                                                                                      aTTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGt                                                                                                                                                                                                     >  1:202214/1‑102 (MQ=255)
                                                                                                                                                                                      aTTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGa                                                                               >  1:874750/1‑220 (MQ=255)
                                                                                                                                                                                                         tATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGac                                                                                                                           >  1:5979/1‑157 (MQ=255)
                                                                                                                                                                                                          aTTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTAc                                                                                         <  1:404235/190‑1 (MQ=255)
                                                                                                                                                                                                          aTTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATg                                                                             >  1:649154/1‑202 (MQ=255)
                                                                                                                                                                                                          aTTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGccc                                                     >  1:333652/1‑226 (MQ=255)
                                                                                                                                                                                                            ttATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAg                           >  1:205535/1‑250 (MQ=255)
                                                                                                                                                                                                                 aTTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGa                                                                >  1:156340/1‑208 (MQ=255)
                                                                                                                                                                                                                       gTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCa                >  1:821610/1‑250 (MQ=255)
                                                                                                                                                                                                                          aaTCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGcc            <  1:76975/251‑1 (MQ=255)
                                                                                                                                                                                                                           aTCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGCCa           >  1:450983/1‑251 (MQ=255)
                                                                                                                                                                                                                                    ccATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTg                             <  1:423481/224‑1 (MQ=255)
                                                                                                                                                                                                                                    ccATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGCCAAGGGTTccg  >  1:202122/1‑251 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
TAAACAATTTGAGCCCTGTGCAGTGCAGAGCTCAATATTTAACTTAGGTTTAGCTATCCGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATGAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGCCAAGGGTTCCG  >  1/158575‑159053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑