| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 980,693 | T→G | I216I (ATA→ATC) | unknown ← | Citrate lyase alpha chain (EC 4.1.3.6) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 980,693 | 0 | T | G | 98.8% | 235.2 / ‑5.2 | 82 | I216I (ATA→ATC) | unknown | Citrate lyase alpha chain (EC 4.1.3.6) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (39/42); minor base A (0/1); total (39/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGGTGATCCCGCCGAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACTATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAGGCAACGTCGATTTTCAGTTCGCCGGATTTCACCAAATGCACGCGAC > 1/980446‑980928 | cGGTGATCCCGCCGAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACgata > 1:497177/1‑251 (MQ=255)cGGTGATCCCGCCGAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACgata > 1:539918/1‑251 (MQ=255) aTCCCGCCGAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGAt > 1:449557/1‑250 (MQ=255) aTCCCGCCGAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGAt < 1:540494/250‑1 (MQ=255) gccgAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACtt > 1:538152/1‑251 (MQ=255) ccgAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACtt < 1:409130/250‑1 (MQ=255) ccgAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTg < 1:570774/251‑1 (MQ=255) aaaGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGAc < 1:93218/251‑1 (MQ=255) aaGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGAc > 1:846589/1‑250 (MQ=255) tCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAGAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGAt < 1:212759/251‑1 (MQ=255) gCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATg > 1:421398/1‑249 (MQ=255) gCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATg > 1:596761/1‑249 (MQ=255) gCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGc < 1:458683/250‑1 (MQ=255) cTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTa < 1:832463/251‑1 (MQ=255) cTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGA‑TTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTa < 1:272791/250‑1 (MQ=255) tAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATc < 1:100298/251‑1 (MQ=255) tAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATc < 1:832726/251‑1 (MQ=255) tAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATc < 1:869511/251‑1 (MQ=255) aTGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCgg > 1:325455/1‑251 (MQ=255) tgttgtCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCggg < 1:43315/251‑1 (MQ=255) ttgtCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGggt < 1:581831/250‑1 (MQ=255) tCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGgtggtg > 1:805371/1‑251 (MQ=255) ccATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATc > 1:449153/1‑236 (MQ=255) ccATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAg < 1:853327/251‑1 (MQ=255) ccATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAg < 1:9406/251‑1 (MQ=255) cATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGt < 1:71227/251‑1 (MQ=255) cttcAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAAt > 1:724828/1‑251 (MQ=255) cAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGAcc > 1:787664/1‑214 (MQ=255) gAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAAttcttc < 1:103520/249‑1 (MQ=255) aaGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACAATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGt < 1:80085/251‑1 (MQ=255) tGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGTACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAac < 1:430637/251‑1 (MQ=255) tgccGCAAGGGGCGCGCCGGCGGTTCCGGTTTGTAAGGAAAAACCGGCTTTGAAAAAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGGCCACGGCATCCCCTTGCACGATAAGATCCCCTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAac < 1:750055/248‑1 (MQ=255) ccGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAacac > 1:499251/1‑250 (MQ=255) ccGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAAcacc < 1:310350/251‑1 (MQ=255) cAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGAcc > 1:555175/1‑196 (MQ=255) aaGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACtt < 1:37622/251‑1 (MQ=255) ggACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACttt < 1:850439/249‑1 (MQ=255) gACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGt < 1:152390/250‑1 (MQ=255) gACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGt < 1:856435/250‑1 (MQ=255) gTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTc > 1:217013/1‑173 (MQ=255) ggTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCAt < 1:90138/248‑1 (MQ=255) ttGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCacac < 1:278970/251‑1 (MQ=255) tAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACggg < 1:577752/251‑1 (MQ=255) aaGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGc < 1:778916/251‑1 (MQ=255) ggAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCAt > 1:808927/1‑251 (MQ=255) gAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATc > 1:199415/1‑174 (MQ=255) aaaaCCGTCTTTGAAATAACCGCAGGCAAAAATCACTTTAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAAc < 1:66058/251‑1 (MQ=255) aaaaCCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAAc < 1:186277/251‑1 (MQ=255) aaaaCCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAAc < 1:457670/251‑1 (MQ=255) tCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCCCTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGa > 1:511685/1‑251 (MQ=255) tCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGa > 1:656875/1‑251 (MQ=255) aaaTAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCcaca > 1:863996/1‑251 (MQ=255) aaaTAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCcaca > 1:267275/1‑251 (MQ=255) ccGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCt < 1:313545/251‑1 (MQ=255) cAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGcc < 1:318184/250‑1 (MQ=255) aaTCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAacac < 1:267333/183‑1 (MQ=255) aCTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGcc < 1:195988/251‑1 (MQ=255) cTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCg < 1:875228/251‑1 (MQ=255) caTTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGAcc > 1:87544/1‑119 (MQ=255) ggCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAAtt > 1:677771/1‑251 (MQ=255) gCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAAcaccac > 1:810328/1‑164 (MQ=255) aaGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCg > 1:899929/1‑250 (MQ=255) gTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCgcag > 1:338841/1‑251 (MQ=255) tAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTAc > 1:280091/1‑236 (MQ=255) cgcgAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGc < 1:488927/251‑1 (MQ=255) ggTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCacac > 1:309531/1‑249 (MQ=255) ggTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCacac > 1:835365/1‑249 (MQ=255) ggTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCacac > 1:601803/1‑249 (MQ=255) ggTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCacac > 1:400858/1‑249 (MQ=255) gTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCg > 1:300016/1‑181 (MQ=255) tggtCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAg < 1:761794/251‑1 (MQ=255) tggtCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAg < 1:12425/251‑1 (MQ=255) ggtCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAAcc < 1:638166/174‑1 (MQ=255) gtCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAgg < 1:810669/250‑1 (MQ=255) gtCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAgg > 1:279244/1‑250 (MQ=255) gTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAGGCAACGTCGAt > 1:874260/1‑251 (MQ=255) ttttttCGGATCGCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAGGCAACGTCGATTTTCAGTTCGCCGGAtt < 1:884506/251‑1 (MQ=255) gCCCACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACAcc > 1:107824/1‑204 (MQ=255) cccACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTtaata > 1:83487/1‑91 (MQ=255) cccACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCg > 1:151322/1‑135 (MQ=255) cccACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCg > 1:363469/1‑135 (MQ=255) cccACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAGGCAACGTCGATTTTCAGTTCGCCGGATTTCACCAAATg > 1:895433/1‑248 (MQ=255) cACGGCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAGGCAACGTCGATTTTCAGTTCGCCGGATTTCACCAAATGCAcg > 1:823663/1‑250 (MQ=255) gCATCCACTTGCACGATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAGGCAACGTCGATTTTCAGTTCGCCGGATTTCACCAAATGCACGCGAc > 1:759362/1‑250 (MQ=255) | CGGTGATCCCGCCGAGGGCAAAGTCGGCTTTAATGTTGTCGCGCACCATTTTTTCTTCAAGGAAGCGGGTGACCGCAAGGGACGCGCCGCCGGTACCGGTTTGTAAGGAAAAACCGTCTTTGAAATAACCGCAGGCAAAAATCACTTCAGCACATTTGCGGGCGATAAGTAATTCGCGAGGGTTGGTGGTCATGCGGGTGGCACCGCCGCCGATTTTTTTCGGATCGCCCACGGCATCCACTTGCACTATAAGATCCACTTGGTCTTGACCGATGCTAATCGGGTGGTGCGGATAGTCGGCGAATTCTTCCGTTAATAACACCACTTTGTCGGCAAATTCCGCATCCACACGGGCATAACCGAGTGAGCCACATTTGCTTTTGCCGCTGAAGCCGTTGGCATTACCGAATTTATCGCAGCAAGGCACACCTAAAAAGGCAACGTCGATTTTCAGTTCGCCGGATTTCACCAAATGCACGCGAC > 1/980446‑980928 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |