Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	888,742	(A)7→6	intergenic (+300/‑155)	unknown → / → unknown	hypothetical protein/Transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	888,736	0	A	.	100.0%	134.0 / NA	43	intergenic (+294/‑161)	unknown/unknown	hypothetical protein/Transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (22/21);  total (22/21)

ATATAAAAAGAAAAAACTTGGTTCCCCTCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGGGGGGGAAATCTCAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAACGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTAAAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGAGGCAATGCCTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCCCTCGTTTGCGCTAAACTAACGGCAATTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGCGAATGATCATAAAATTTTAGCCAATATCC  >  1/888488‑888966                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       atataAAAAGAAAAAACTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaa                                                                                                                                                                                                                                      <  1:252/251‑1 (MQ=14)              aaaCTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGaa                                                                                                                                                                                                                        <  1:62052/251‑1 (MQ=14)                aCTTGGCTCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAAtt                                                                                                                                                                                                                      >  1:879846/1‑251 (MQ=14)                      ctCCCCCCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATg                                                                                                                                                                                                                <  1:261645/250‑1 (MQ=14)                            ccGTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGa                                                                                                                                                                                                          >  1:6479/2‑251 (MQ=14)                              gTTCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATaa                                                                                                                                                                                                                  <  1:389946/241‑1 (MQ=14)                                tCACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGaacaa                                                                                                                                                                                                      >  1:668103/1‑251 (MQ=255)                                 cACGGGGGAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaaaa                                                                                                                                                                                                                                    <  1:598963/221‑1 (MQ=255)                                     ggggAGCTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTa                                                                                                                                                                                                 >  1:740927/1‑251 (MQ=255)                                           cTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATAccc                                                                                                                                                                                           <  1:392023/251‑1 (MQ=255)                                           cTGGGCGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATAc                                                                                                                                                                                             <  1:558716/249‑1 (MQ=14)                                                cGAAGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGc                                                                                                                                                                                      <  1:493436/251‑1 (MQ=14)                                                   aGCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTaaa                                                                                                                                                                                                                                      <  1:329334/201‑1 (MQ=255)                                                    gCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATaa                                                                                                                                                                                                                  <  1:818221/219‑1 (MQ=11)                                                    gCCCTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATg                                                                                                                                                                                  >  1:368847/1‑251 (MQ=14)                                                     cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACTCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATg                                                                                                                                                                                  >  1:126165/1‑250 (MQ=14)                                                     cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc                                                                                                                                                                                 <  1:705025/251‑1 (MQ=14)                                                     cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc                                                                                                                                                                                 <  1:50152/251‑1 (MQ=14)                                                     cccTGAGTGGGCAAAATCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGc                                                                                                                                                                                 <  1:845409/251‑1 (MQ=14)                                                            tgggcaaaaTCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                                                                                          <  1:824935/245‑1 (MQ=14)                                                                 aaaaTCTAAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGt                                                                                                                                                                     <  1:679621/250‑1 (MQ=255)                                                                        aaaTTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTa                                                                                                                                                              <  1:621061/250‑1 (MQ=255)                                                                                aaGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGttt                                                                                                                                                                                                             >  1:87613/1‑196 (MQ=255)                                                                                         cTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCATTGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGa                                                                                                                                             >  1:686507/1‑251 (MQ=255)                                                                                                  aCATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTg                                                                                                                                    <  1:807303/251‑1 (MQ=255)                                                                                                   cATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGaac                                                                                                                                                                                                        <  1:694365/182‑1 (MQ=255)                                                                                                   cATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAAt                                                                                                                                                                >  1:551652/1‑222 (MQ=255)                                                                                                        aGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTtata                                                                                                                                                 >  1:520546/1‑232 (MQ=255)                                                                                                        aGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAAt                                                                                                                               >  1:357275/1‑250 (MQ=255)                                                                                                                                        ttATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAAc                                                                                                           <  1:754674/238‑1 (MQ=21)                                                                                                                                          aTTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                                                                                          >  1:540947/1‑173 (MQ=255)                                                                                                                                                  gTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTAct                                                                                     >  1:454584/1‑250 (MQ=25)                                                                                                                                                   ttCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTAct                                                                                     >  1:123398/1‑249 (MQ=25)                                                                                                                                                                       ccccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAActg                                                                                                     >  1:440748/1‑211 (MQ=25)                                                                                                                                                                         ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGt                                                                                                                                                                     <  1:629812/147‑1 (MQ=18)                                                                                                                                                                         ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTgg                                                                                                                                   >  1:285552/1‑181 (MQ=255)                                                                                                                                                                         ccTTTGGGGGCGTTGGCAGAGCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCa                                                             >  1:598920/1‑251 (MQ=37)                                                                                                                                                                                            agCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATAc                                                                                                                                                >  1:590411/1‑149 (MQ=21)                                                                                                                                                                                             gCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                                                                                          >  1:16384/1‑122 (MQ=18)                                                                                                                                                                                             gCCAATGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTAt                                                                                                                                      <  1:607509/158‑1 (MQ=25)                                                                                                                                                                                                        aaaCGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAgtg                              <  1:94864/251‑3 (MQ=38)                                                                                                                                                                                                                 gCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGccc                                                                                                                                                                          >  1:616685/1‑102 (MQ=18)                                                                                                                                                                                                                       tGTCCCCCCGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAAtt               <  1:793962/251‑1 (MQ=39)                                                                                                                                                                                                                             cccGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGAt                        >  1:616048/1‑236 (MQ=39)                                                                                                                                                                                                                             cccGTAAACGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGc          >  1:790940/1‑250 (MQ=255)                                                                                                                                                                                                                                    aCGGAGGAAGGCAAGATATTT‑AAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGGGGCAATGCTTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCTCTTGTTTGCGCTAAACTAACTGCAACTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGTGAATGATCATAAAATTTTAGCCAATATcc  <  1:849332/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       ATATAAAAAGAAAAAACTTGGTTCCCCTCGTTCACGGGGGAGCTGGGCGAAGCCCTGAGGGGGGGAAATCTCAATTTTTAAGCATGATCTTGTGAAAACATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACGTTATTCGCAAGTTCGTAAACTTACTTGCTCACCCCTTTGGGGGCGTTGGCAGAGCCAACGCTCAAAACGCAATGCGTTTTGTCCCCCCGTAAACGGAGGAAGGCAAGATATTTAAAAAAATATCAATGAATTATAATGTTTCGAACAACTGTAATACCCGAGGCAATGCCTTGCCCTAGGTAGAATTACTGTCTTTTTATACAGATCTTTATTGGAAATCCCCCTCGTTTGCGCTAAACTAACGGCAATTTTTTTCTTACTCTTTTGTCGTTTTATGCAAACTCAATTTTTTCATCTCGAAATTCCTACCCAAGCGAATGATCATAAAATTTTAGCCAATATCC  >  1/888488‑888966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑