Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	92,929	G→C	P315A (CCG→GCG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	92,929	0	G	C	90.0%	20.9 / ‑3.9	10	P315A (CCG→GCG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (8/1);  total (8/2)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCGGGCTGTACCTCAATACGCACACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGTATATTTTCCGGCTGCAATCGGATAAACGGTGGTTAAAGTGCGGTCATAATTGCCCACGACCGCGCGTTTGAGCTTGATTTTGTTGGATTCAAGCGCACTAATCTCGACCATTTCTTTGTTTTTTCCTGTAATCAAAATGGCACGGCCACCGACGGCAAAATCATAGCCTGCGGTGTTAATAGCAAGCTCTGTGGCGCCCTGTTGCACATTTTGCAACAACTGGGCGCTGTCGGTAAAAATCGGCATCGACCACACGCGCGAGC  >  1/92688‑93171                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   atgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgtt                                                                                                                                                                                                                                           >  1:454507/7‑246 (MQ=255) atgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgtt                                                                                                                                                                                                                                           >  1:691605/7‑246 (MQ=255)  tgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttg                                                                                                                                                                                                                                          >  1:662036/6‑245 (MQ=255)   gcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgt                                                                                                                                                                                                                                         >  1:813282/5‑244 (MQ=255)       ggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaat                                                                                                                                                                                                                                     >  1:282358/1‑240 (MQ=255)        gCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaat                                                                                                                                                                                                                                     <  1:458378/250‑12 (MQ=255)              ccTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaatatcggta                                                                                                                                                                                                                              >  1:216301/1‑233 (MQ=255)                tCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaatatcggtagt                                                                                                                                                                                                                            >  1:552138/1‑231 (MQ=255)                                                                 cTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaata                                                                                                                                                                                                                                    >  1:335570/1‑182 (MQ=255)                                                                                                                                                                                                                                          ccgcagcGGATAAACGGTGTTTAAAGTGCGCTAATATTTCCCCACGCCCGCGCGTTTGAGTTTGTTTTTCCTCGGTTCAGGGGCGTTGTTTTCGACCTTTTTTTTGTTGCTTGCGGTCATAAAAACCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGc  <  1:653339/245‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   ACGCCGGGCTGTACCTCAATACGCACACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGTATATTTTCCGGCTGCAATCGGATAAACGGTGGTTAAAGTGCGGTCATAATTGCCCACGACCGCGCGTTTGAGCTTGATTTTGTTGGATTCAAGCGCACTAATCTCGACCATTTCTTTGTTTTTTCCTGTAATCAAAATGGCACGGCCACCGACGGCAAAATCATAGCCTGCGGTGTTAATAGCAAGCTCTGTGGCGCCCTGTTGCACATTTTGCAACAACTGGGCGCTGTCGGTAAAAATCGGCATCGACCACACGCGCGAGC  >  1/92688‑93171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑