Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	92,912	T→C	K320Q (AAA→CAG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	92,912	0	T	C	100.0%	33.4 / NA	13	K320Q (AAA→CAG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/1);  total (12/1)

TGCCAGGGCATTGCGGCTAACGCCGGGCTGTACCTCAATACGCACACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGTATATTTTCCGGCTGCAATCGGATAAACGGTGGTTAAAGTGCGGTC  >  1/92669‑92953                                                                                                                                                                                                                                                    |                                          tGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCgg                                    >  1:591920/1‑251 (MQ=255) tGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCgg                                    >  1:701139/1‑251 (MQ=255)          gttGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCg                           >  1:588685/2‑251 (MQ=255)                    atgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgtt                 >  1:454507/7‑246 (MQ=255)                    atgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgtt                 >  1:691605/7‑246 (MQ=255)                     tgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttg                >  1:662036/6‑245 (MQ=255)                      gcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgt               >  1:813282/5‑244 (MQ=255)                          ggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaat           >  1:282358/1‑240 (MQ=255)                           gCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCg                           >  1:202598/1‑234 (MQ=255)                           gCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaat           <  1:458378/250‑12 (MQ=255)                                 ccTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaatatcggta    >  1:216301/1‑233 (MQ=255)                                   tCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaatatcggtagt  >  1:552138/1‑231 (MQ=255)                                                                                    cTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaata          >  1:335570/1‑182 (MQ=255)                                                                                                                                                                                                                                                    |                                          TGCCAGGGCATTGCGGCTAACGCCGGGCTGTACCTCAATACGCACACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGTATATTTTCCGGCTGCAATCGGATAAACGGTGGTTAAAGTGCGGTC  >  1/92669‑92953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑