Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	92,908	T→C	T322A (ACG→GCG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	92,908	0	T	C	100.0%	33.7 / NA	13	T322A (ACG→GCG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/1);  total (12/1)

CCCTGAATGCCAGGGCATTGCGGCTAACGCCGGGCTGTACCTCAATACGCACACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGTATATTTTCCGGCTGCAATCGGATAAACGGTGGTTAAAGTGCGGTC  >  1/92662‑92953                                                                                                                                                                                                                                                       |                                              cccTAAATGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcatac                                           <  1:139929/251‑2 (MQ=255) cccTAAATGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcatac                                           <  1:528029/251‑2 (MQ=255)  cctaaaTGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcata                                            >  1:187327/5‑249 (MQ=255)  cctaaaTGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcata                                            >  1:523551/5‑249 (MQ=255)   ctaaaTGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcata                                            >  1:709256/4‑248 (MQ=255)   ctaaaTGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcata                                            >  1:138103/4‑248 (MQ=255)    taaaTGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcata                                            >  1:794148/3‑247 (MQ=255)        tGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCgg                                    >  1:701139/1‑251 (MQ=255)        tGCCAGCGCGTTGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCgg                                    >  1:591920/1‑251 (MQ=255)                 gttGCGGCTAATGCCCGGCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCg                           >  1:588685/2‑251 (MQ=255)                           atgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgtt                 >  1:691605/7‑246 (MQ=255)                           atgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgtt                 >  1:454507/7‑246 (MQ=255)                            tgcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttg                >  1:662036/6‑245 (MQ=255)                             gcccggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgt               >  1:813282/5‑244 (MQ=255)                                 ggCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaat           >  1:282358/1‑240 (MQ=255)                                  gCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACACAGCGCGcatac                                           >  1:62954/1‑217 (MQ=255)                                  gCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcatactgtc                                       >  1:770910/1‑217 (MQ=255)                                  gCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCg                           >  1:202598/1‑234 (MQ=255)                                  gCTGTACCTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaat           <  1:458378/250‑12 (MQ=255)                                        ccTCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaatatcggta    >  1:216301/1‑233 (MQ=255)                                          tCAATGCGCAGACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaatatcggtagt  >  1:552138/1‑231 (MQ=255)                                                                                           cTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGCATACTGTCCGGCTGCAATCGCATaatcgttgtaaata          >  1:335570/1‑182 (MQ=255)                                                                                                                                              ggTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGcatactgtcc                                      <  1:185450/115‑7 (MQ=255)                                                                                                                                                                                                                                                       |                                              CCCTGAATGCCAGGGCATTGCGGCTAACGCCGGGCTGTACCTCAATACGCACACGAATTTTATCCACATTTTGCGCGAGGCTGTCAGCCACTTGTTGCGGATGCCAATCGTCAGTTGTCCAGTCGCCCAAAAGCTCGGATGGGTATGATTTGACCACCTCGGCGCCGCTTAAAAGCTGCACAATCACCCGGCATTTGCCTTTGCGCTGCGCATAGCTCAGTATCTTATAATCAAGCACAAAGCGCGTATATTTTCCGGCTGCAATCGGATAAACGGTGGTTAAAGTGCGGTC  >  1/92662‑92953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑