Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	849,077	A→G	S462S (TCT→TCC)	unknown ←	ABC transporter, dimeric ATP‑binding protein PM1326

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	849,077	0	A	G	100.0%	211.4 / NA	74	S462S (TCT→TCC)	unknown	ABC transporter, dimeric ATP‑binding protein PM1326
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/30);  total (44/30)

TTGTAATAGGATATTAAAAATATTGTTAGTCGATAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCAGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACGATATGTAG  >  1/848831‑849319                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ttGTAATAGGATATTAAAAATATTGTTAGTCGATAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGaaa                                                                                                                                                                                                                                                >  1:892983/1‑251 (MQ=255)   gTAATAGGATATTAAAAATATTGTTAGTCGATAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATg                                                                                                                                                                                                                                              >  1:67979/1‑251 (MQ=255)             atTAAAAATATTGTTAGTCGATATAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAAtt                                                                                                                                                                                                                                    >  1:553876/1‑251 (MQ=255)                             gTCGATAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCcgacga                                                                                                                                                                                                                    >  1:315164/1‑251 (MQ=255)                                 aTAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATAc                                                                                                                                                                                                                 >  1:617224/1‑250 (MQ=255)                                 aTAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATAc                                                                                                                                                                                                                 >  1:32420/1‑250 (MQ=255)                                 aTAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATAc                                                                                                                                                                                                                 >  1:856579/1‑250 (MQ=255)                                 aTAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTCTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATAc                                                                                                                                                                                                                 <  1:448176/250‑1 (MQ=255)                                          cTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGAt                                                                                                                                                                                                        <  1:189543/250‑1 (MQ=255)                                          cTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGAt                                                                                                                                                                                                        <  1:186382/250‑1 (MQ=255)                                          cTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGAt                                                                                                                                                                                                        <  1:885479/250‑1 (MQ=255)                                          cTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATa                                                                                                                                                                                                       >  1:242832/1‑251 (MQ=255)                                                              ccTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAg                                                                                                                                                                                                      <  1:504996/232‑1 (MQ=255)                                                                                 aaaCTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGAt                                                                                                                                                                                                        >  1:597593/1‑211 (MQ=255)                                                                                 aaaCTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATaa                                                                                                                                                                >  1:233519/1‑251 (MQ=255)                                                                                              gCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTg                                                                                                                                                   >  1:376841/1‑251 (MQ=255)                                                                                                              attTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGAc                                                                                                                                                       <  1:89563/231‑1 (MQ=255)                                                                                                                            aaaCATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTCCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTtcat                                                                                                                     >  1:748068/1‑251 (MQ=255)                                                                                                                             aaCATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTtcat                                                                                                                     >  1:95219/1‑250 (MQ=255)                                                                                                                              aCATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTtcatca                                                                                                                   >  1:566481/1‑251 (MQ=255)                                                                                                                              aCATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTtcatca                                                                                                                   >  1:729462/1‑251 (MQ=255)                                                                                                                              aCATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTtcatca                                                                                                                   >  1:411470/1‑251 (MQ=255)                                                                                                                                   gtgtCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGt                                                                                                                                                                                      <  1:756251/179‑1 (MQ=255)                                                                                                                                     gtCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATc                                                                                                                                                                                                                          <  1:471525/141‑1 (MQ=255)                                                                                                                                         tCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGAt                                                                                                                                                                                                        <  1:713486/155‑1 (MQ=255)                                                                                                                                         tCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAAt                                                                                                                                                              <  1:678873/197‑1 (MQ=255)                                                                                                                                         tCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGc                                                                                                                                    >  1:890747/1‑223 (MQ=255)                                                                                                                                         tCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAat                                                                                                        >  1:54972/1‑251 (MQ=255)                                                                                                                                         tCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAat                                                                                                        >  1:26408/1‑251 (MQ=255)                                                                                                                                         tCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAat                                                                                                        >  1:577507/1‑251 (MQ=255)                                                                                                                                         tCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAat                                                                                                        <  1:695982/251‑1 (MQ=255)                                                                                                                                         tCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAat                                                                                                        >  1:85547/1‑251 (MQ=255)                                                                                                                                            ttATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCaa                                                                                                                  >  1:459594/1‑238 (MQ=255)                                                                                                                                            ttATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATc                                                                                                     <  1:826545/251‑1 (MQ=255)                                                                                                                                               ttCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAAtt                                                                                                                                                                                 >  1:352528/1‑172 (MQ=255)                                                                                                                                               ttCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTc                                                                                                                                                           <  1:42551/194‑1 (MQ=255)                                                                                                                                               ttCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGata                                                                                                                                           <  1:12824/210‑1 (MQ=255)                                                                                                                                                tCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAat                                                                                                        <  1:732500/244‑1 (MQ=255)                                                                                                                                                 cTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTgc                                                                                                                                                                                                   <  1:559863/152‑1 (MQ=255)                                                                                                                                                 cTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCAcc                                                                                                 >  1:644715/1‑250 (MQ=255)                                                                                                                                                        ccTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTgc                                                                                                                                                                                                   <  1:505765/145‑1 (MQ=255)                                                                                                                                                        ccTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGc                                                                                             <  1:845538/247‑1 (MQ=255)                                                                                                                                                        ccTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCttt                                                                                          >  1:145514/1‑250 (MQ=255)                                                                                                                                                           atcatcAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTgg                                                                                                                                                                               >  1:891031/1‑162 (MQ=255)                                                                                                                                                           atcatcAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTaa                                                                                      >  1:683087/1‑251 (MQ=255)                                                                                                                                                            tcatcaGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAAc                                                                                     <  1:391458/251‑1 (MQ=255)                                                                                                                                                                          aaaTTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAAt                                                                                                                                      >  1:693238/1‑188 (MQ=255)                                                                                                                                                                             ttttCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCAc                                                                    <  1:803028/251‑1 (MQ=255)                                                                                                                                                                                 cttcCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTc                                                                <  1:774603/251‑1 (MQ=255)                                                                                                                                                                                       tAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATCCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTctgctg                                                           <  1:425870/250‑1 (MQ=255)                                                                                                                                                                                                        gTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAAt                                                                                                                                      <  1:240076/158‑1 (MQ=255)                                                                                                                                                                                                              tCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGt                                   >  1:657489/1‑251 (MQ=255)                                                                                                                                                                                                              tCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGt                                   >  1:167858/1‑251 (MQ=255)                                                                                                                                                                                                                    aTCTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAg                                                                                                                                                                                                      >  1:784135/1‑82 (MQ=255)                                                                                                                                                                                                                      cTTTTCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGTGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGtt                           >  1:813246/1‑251 (MQ=255)                                                                                                                                                                                                                        tttCACCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTc                          >  1:357365/1‑250 (MQ=255)                                                                                                                                                                                                                            aCCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGAt                     <  1:225743/251‑1 (MQ=255)                                                                                                                                                                                                                            aCCGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGAt                     <  1:380924/251‑1 (MQ=255)                                                                                                                                                                                                                             ccGTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGAt                     >  1:2891/1‑250 (MQ=255)                                                                                                                                                                                                                               gTTACGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTtc                                                                                                                       >  1:312108/1‑150 (MQ=255)                                                                                                                                                                                                                                  aCGCATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTgg                                    <  1:779317/230‑1 (MQ=255)                                                                                                                                                                                                                                     cATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGaaa            >  1:410359/1‑251 (MQ=255)                                                                                                                                                                                                                                     cATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGaaa            >  1:37872/1‑251 (MQ=255)                                                                                                                                                                                                                                     cATTACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGaaa            >  1:464238/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACg          >  1:231611/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttACTACGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACg          >  1:582044/1‑251 (MQ=255)                                                                                                                                                                                                                                         actacGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACtt                                                                  >  1:783619/1‑193 (MQ=255)                                                                                                                                                                                                                                         actacGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACGat        >  1:253613/1‑251 (MQ=255)                                                                                                                                                                                                                                         actacGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACGat        >  1:225210/1‑251 (MQ=255)                                                                                                                                                                                                                                         actacGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACGat        >  1:479807/1‑251 (MQ=255)                                                                                                                                                                                                                                             cGATGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACGATATGt    <  1:21417/251‑1 (MQ=255)                                                                                                                                                                                                                                               aTGCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACGATATGTAg  <  1:175110/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gCGATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGaaa            <  1:815182/239‑1 (MQ=255)                                                                                                                                                                                                                                                   gATCGGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACGATATGTAg  <  1:797192/247‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   TTGTAATAGGATATTAAAAATATTGTTAGTCGATAAAAAGGCTTCTGATGTGAAAGACATTCCTAAAATCATGAAAAATAAAACTAACCCGATGCCTAATTTGGAGATTATTTTTTTCAATGAAAACATAGTGTCATCCTTATTCTAAACACCTATCATCAGGTGCATTAAATTTTCTTCCGTAATTTCGGTGCGATCGGTGATTTCTTTTATCTTTTCACCGTTACGCATTACTACGATGCGATCAGAAATGGTGATTAATTCCGGTAAATCCGACGATACCATCACGATAGTGCCGCCGCTTTCGGTAAATTGGCGCATTAAATCATAAATTTCCGACTTTGCCCCGATATCAATGCCGCGAGTCGGTTCATCAAAAAACAAAATATCCACCCCGCTTTCTAACCAGCGAGCCAAAATCACTTTCTGCTGATTTCCGCCCGATAAGGTTTTGGTTTGCTGTTCCTGATCGTGAGAAACGATATGTAG  >  1/848831‑849319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑