Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	738,970	G→A	G32S (GGC→AGC)	unknown →	Mobile element protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	738,970	0	G	A	100.0%	128.7 / NA	64	G32S (GGC→AGC)	unknown	Mobile element protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/28);  total (36/28)

CCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATGGAAAACGAGGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAATGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCGTTGATGGATTTAGCGAACCG  >  1/738727‑739115                                                                                                                                                                                                                                                    |                                                                                                                                                  cccATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAAc                                                                                                                                            <  1:333872/251‑1 (MQ=255)             tCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGc                                                                                                                                <  1:225965/251‑1 (MQ=255)              cATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGc                                                                                                                                >  1:493687/1‑250 (MQ=255)               aTTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGccc                                                                                                                              <  1:668204/251‑1 (MQ=255)                    ttGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAAt                                                                                                                         >  1:519184/1‑251 (MQ=25)                     tGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAAtg                                                                                                                        <  1:732084/251‑1 (MQ=25)                      ggCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAAtgt                                                                                                                       >  1:155350/1‑251 (MQ=21)                       gCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAAtgtg                                                                                                                      >  1:492684/1‑251 (MQ=21)                         tAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCt                                                                                                                    <  1:775324/251‑1 (MQ=18)                               cctttCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAAt                                                                                                                <  1:210197/247‑1 (MQ=11)                               cctttCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGACTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCg                                                                                                              >  1:48009/3‑251 (MQ=2)                               cATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAAt                                                                                                                <  1:638162/249‑1 (MQ=17)                               cATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAAt                                                                                                                <  1:737050/249‑1 (MQ=17)                               cATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAAt                                                                                                                <  1:526596/249‑1 (MQ=17)                                     gTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGAttt                                                                                                        >  1:725765/1‑251 (MQ=14)                                            agGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAg                                                                                                   <  1:744166/249‑1 (MQ=14)                                               gCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGAAAAACATCGCATATTGccc                                                                                                                              >  1:436411/1‑219 (MQ=9)                                                         gTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCtcaat                                                                                     >  1:882187/1‑250 (MQ=14)                                                              aTCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCaaaag                                                                               <  1:465967/251‑2 (MQ=14)                                                               tCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGGATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAAt                                                                              <  1:558898/251‑1 (MQ=14)                                                                 cccTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAAc                                                                                                                                            >  1:784933/1‑187 (MQ=17)                                                                 cccTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCt                                                                                                                    <  1:90513/211‑1 (MQ=14)                                                                 cccTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAAt                                                                                                                >  1:246880/1‑215 (MQ=14)                                                                  ccTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTggg                                                                           <  1:510499/251‑1 (MQ=14)                                                                  ccTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGAGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTggg                                                                           <  1:218177/251‑1 (MQ=9)                                                                   cTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGt                                                                          <  1:669858/251‑1 (MQ=14)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCata                                                                                                                                    >  1:218485/1‑190 (MQ=17)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATAtt                                                                                                                                  >  1:771067/1‑192 (MQ=17)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAAt                                                                                                                         <  1:310059/201‑1 (MQ=14)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTaa                                                                        >  1:771553/1‑250 (MQ=14)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTaa                                                                        >  1:366529/1‑250 (MQ=14)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTaa                                                                        >  1:901454/1‑250 (MQ=14)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTaa                                                                        >  1:365770/1‑250 (MQ=14)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTaa                                                                        >  1:222304/1‑250 (MQ=14)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAATGGGTaa                                                                        >  1:851427/1‑250 (MQ=14)                                                                      atataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGCATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCat                                                                                                                                     >  1:868606/1‑189 (MQ=12)                                                                        ataAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCg                                                                     <  1:663511/251‑1 (MQ=14)                                                                             cccTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCg                                                                                                              >  1:262570/1‑205 (MQ=14)                                                                             cccTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCaaaag                                                                               >  1:578190/1‑235 (MQ=14)                                                                             cccTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGt                                                                 >  1:481328/1‑250 (MQ=14)                                                                                      cccTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGttt                                                       >  1:62761/1‑251 (MQ=14)                                                                                      cccTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCACat                                                                                                                                     >  1:443540/1‑173 (MQ=12)                                                                                       ccTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAATggg                                                                           >  1:37554/1‑230 (MQ=14)                                                                                        cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCg                                                     >  1:359153/1‑251 (MQ=14)                                                                                        cTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAATGGGTAACCGATGTCAc                                                              <  1:554589/242‑1 (MQ=14)                                                                                          aaaaTAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACAt                                                                                                                                          <  1:706359/164‑1 (MQ=17)                                                                                          aaaaTAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTg                                                            <  1:607384/242‑1 (MQ=14)                                                                                           aaaTAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGt                                                  <  1:219501/251‑1 (MQ=14)                                                                                                     gCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTa                                                                                                                   >  1:714138/1‑176 (MQ=17)                                                                                                      ccAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGaaa                                       <  1:463951/251‑1 (MQ=14)                                                                                                           gATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGgaagaa                                        <  1:464094/245‑1 (MQ=14)                                                                                                           gATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGct                                   >  1:382515/1‑250 (MQ=14)                                                                                                           gATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGAGCTAAATCGTGATTTTAc                                                                                                     >  1:460359/1‑184 (MQ=12)                                                                                                                aaaaGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTAttt                             <  1:899815/251‑1 (MQ=14)                                                                                                                       agCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCg                      >  1:553444/1‑251 (MQ=14)                                                                                                                        gCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAATGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCGt                     >  1:863768/1‑251 (MQ=14)                                                                                                                               aaaTCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCGTTGATGGa              <  1:692983/251‑1 (MQ=14)                                                                                                                                aaTCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCGTTGATGGAt             <  1:10336/251‑1 (MQ=14)                                                                                                                                  tCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTg                                                            >  1:573677/1‑202 (MQ=9)                                                                                                                                  tCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCGt                     >  1:285616/1‑241 (MQ=14)                                                                                                                                       gAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCaaaagt                                                                              >  1:738673/1‑177 (MQ=12)                                                                                                                                          cAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCGTTGATGGATTTAGCGAAc    >  1:234831/1‑250 (MQ=14)                                                                                                                                           aaaCTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCCAAAGTGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCGTTGATGGATTTAGCGAACCg  >  1:442241/1‑251 (MQ=9)                                                                                                                                            aaCTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATAGAAAACGAAGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAATGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTAttt                             <  1:30635/223‑1 (MQ=14)                                                                                                                                                                                                                                                    |                                                                                                                                                  CCCATTCGCTCGTCATTTATTGGCTAAAAGCATTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACGCAAGAAAAGACATGGAAAACGAGGCAAAACATCGCATATTGCCCCGAATGTGCTAAATCGTGATTTTACAGCGACGGCGCTCAATCAAAAATGGGTAACCGATGTCACTGAGTTTCGAGTTGGGGAAGAAAAGCTCTATTTTTCACCGTTGATGGATTTAGCGAACCG  >  1/738727‑739115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑