BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	724,359	G→A	G82E (GGA→GAA)	unknown →	Transposase, IS200 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	724,359	0	G	A	100.0%	100.6 / NA	40	G82E (GGA→GAA)	unknown	Transposase, IS200 family
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/23); total (17/23)

GGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGACAGTGGAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGGATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCAGTAAGTAAACAAAGATTATTATAGTTTGTT  >  1/724256‑724584

ggCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGAt                                                                                >  1:203148/1‑251 (MQ=2)

                cGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGgtg                                                                >  1:901509/1‑251 (MQ=255)

                  gTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTa                                                              >  1:239399/1‑251 (MQ=255)

                      ggCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAttt                                                          >  1:88441/1‑251 (MQ=255)

                      ggCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAt                                                            <  1:173696/249‑1 (MQ=255)

                            aTACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTAcc                                                      <  1:91189/249‑1 (MQ=255)

                               caaaggtaGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGaa                                                  >  1:555476/8‑250 (MQ=255)

                                       gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACg                                                                                                     <  1:566414/187‑1 (MQ=17)

                                       gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTg                                                                                                   <  1:619250/189‑1 (MQ=17)

                                       gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTg                                                                                                   <  1:235146/189‑1 (MQ=17)

                                       gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTg                                           >  1:654396/5‑249 (MQ=255)

                                       gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGtt                                         >  1:178769/5‑251 (MQ=255)

                                       gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGtt                                         >  1:74541/5‑251 (MQ=255)

                                         gtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGt                                                                                                                             >  1:518386/3‑165 (MQ=14)

                                           aggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTAttt                                                                                      <  1:871578/202‑1 (MQ=18)

                                            ggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGtt                                     >  1:308845/1‑250 (MQ=255)

                                             gagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGtt                                     <  1:830410/249‑1 (MQ=255)

                                              agTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAttt                                                          <  1:224001/227‑1 (MQ=255)

                                               gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGt                                                                                                                             <  1:155703/159‑1 (MQ=17)

                                               gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCAc                                                                                                             <  1:140300/175‑1 (MQ=21)

                                               gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGAt                                                                                              <  1:59046/190‑1 (MQ=21)

                                               gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTg                                                                                            <  1:413476/192‑1 (MQ=21)

                                               gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAt                                                            <  1:527362/224‑1 (MQ=255)

                                               gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCgg                                  >  1:444977/1‑250 (MQ=255)

                                                tatataAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGc                                 <  1:315518/250‑1 (MQ=255)

                                                 atataAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTa                                                                                    >  1:131856/1‑198 (MQ=25)

                                                 atataAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCag                               <  1:299360/251‑1 (MQ=255)

                                                 atacaaGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCag                               >  1:800039/5‑251 (MQ=255)

                                                        gAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGaactaact                                            <  1:359192/231‑1 (MQ=255)

                                                        gAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCagtaagt                          <  1:127330/249‑1 (MQ=255)

                                                            cAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACg                                                                                                     >  1:23807/1‑170 (MQ=25)

                                                                aaaaGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCAGTAAGTAAACAAAGa                 <  1:5896/250‑1 (MQ=255)

                                                                    ggAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAAttt                                                          <  1:525547/205‑1 (MQ=255)

                                                                      aaGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTGGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCAGTAAGTAAACAAAGATtattat          <  1:519128/251‑1 (MQ=255)

                                                                              aTTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATag                                                                                                                                >  1:98280/1‑125 (MQ=18)

                                                                              aTTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTg                                           >  1:171817/1‑210 (MQ=255)

                                                                              aTTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCAGTAAGTAAACAAAGATTATTATAgtttgtt  >  1:631322/1‑251 (MQ=255)

                                                                                   ggATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCg                                   <  1:142111/213‑1 (MQ=255)

                                                                                       aaTTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGt                                                                                                                             <  1:604727/119‑1 (MQ=18)

                                                                                        aTTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCAGTAAGTAAACAAAGatta              <  1:108958/229‑1 (MQ=255)

GGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGACAGTGGAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGGATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTATTTTATGATTTTTTTGTACAGGGTGTAATTTTACCTGAACTAACTGTTAGTTCGGCAGTAAGTAAACAAAGATTATTATAGTTTGTT  >  1/724256‑724584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`