Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	706,589	A→G	T119A (ACG→GCG)	unknown →	Late competence protein ComEA, DNA receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	706,589	0	A	G	100.0%	229.2 / NA	78	T119A (ACG→GCG)	unknown	Late competence protein ComEA, DNA receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/50);  total (28/50)

ACCGTTAAATCCGATGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTACGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGAAAAAGTCAAATCCCATGTGCGTTTTAACTAATCTTGACG  >  1/706352‑706824                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            aCCGTTAAATCCGATGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATaa                                                                                                                                                                                                                                <  1:65276/251‑1 (MQ=255) aCCGTTAAATCCGATGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATaa                                                                                                                                                                                                                                >  1:738271/1‑251 (MQ=255)  ccGTTAAATCCGATGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATaaa                                                                                                                                                                                                                               >  1:484321/1‑251 (MQ=255)  ccGTTAAATCCGATGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATaaa                                                                                                                                                                                                                               >  1:701494/1‑251 (MQ=255)    gTTAAATCCGATGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAg                                                                                                                                                                                                                              >  1:662188/1‑250 (MQ=255)    gTTAAATCCGATGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAg                                                                                                                                                                                                                              >  1:702981/1‑250 (MQ=255)               tGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATcaca                                                                                                                                                                                                                   >  1:662671/1‑250 (MQ=255)                      aCCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGcc                                                                                                                                                                                                                            >  1:855461/1‑234 (MQ=255)                      aCCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCaa                                                                                                                                                                                                           >  1:48815/1‑251 (MQ=255)                          tGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCaaaaa                                                                                                                                                                                                        <  1:823649/250‑1 (MQ=255)                          tGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCaaaaa                                                                                                                                                                                                        <  1:792702/250‑1 (MQ=255)                          tGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCaaaaa                                                                                                                                                                                                        <  1:319336/250‑1 (MQ=255)                            ggCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAAt                                                                                                                                                                                                                                  <  1:302613/222‑1 (MQ=255)                                gTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAAt                                                                                                                                                                                                                       >  1:607740/1‑229 (MQ=255)                                gTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGat                                                                                                                                                                                                 >  1:729782/1‑251 (MQ=255)                                     gTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATaa                                                                                                                                                                                                                                <  1:117206/215‑1 (MQ=255)                                     gTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTaa                                                                                                                                                                                            >  1:270938/1‑251 (MQ=255)                                     gTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTaa                                                                                                                                                                                            >  1:790481/1‑251 (MQ=255)                                      tGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAac                                                                                                                                                                                           <  1:216414/251‑1 (MQ=255)                                        aaaGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAacac                                                                                                                                                                                         <  1:407263/251‑1 (MQ=255)                                        aaaGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAacac                                                                                                                                                                                         <  1:490315/251‑1 (MQ=255)                                        aaaGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAacac                                                                                                                                                                                         <  1:13975/251‑1 (MQ=255)                                        aaaGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAacac                                                                                                                                                                                         <  1:854273/251‑1 (MQ=255)                                        aaaGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAacac                                                                                                                                                                                         <  1:387563/251‑1 (MQ=255)                                        aaaGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAacac                                                                                                                                                                                         <  1:154410/251‑1 (MQ=255)                                                  cgGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCa                                                                                                                                                                               >  1:682970/1‑251 (MQ=255)                                                    gTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAac                                                                                                                                                                                           >  1:843848/1‑237 (MQ=255)                                                        aaGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACt                                                                                                                                                                         <  1:291010/251‑1 (MQ=255)                                                          gTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATc                                                                                                                                                                                                                      <  1:739347/204‑1 (MQ=255)                                                                         aaaGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCgg                                                                                                                                                        >  1:324015/1‑251 (MQ=255)                                                                             agaAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTAt                                                                                                                                                     <  1:373224/250‑1 (MQ=255)                                                                             agaAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTAt                                                                                                                                                     <  1:248771/250‑1 (MQ=255)                                                                                 aTTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTg                                                                                                                                                <  1:367936/251‑1 (MQ=255)                                                                                 aTTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTg                                                                                                                                                <  1:786613/251‑1 (MQ=255)                                                                                      ttGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGTGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCa                                                                                                                                           <  1:452776/251‑1 (MQ=255)                                                                                                         tAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGGCACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTgg                                                                                                                        <  1:670916/251‑1 (MQ=255)                                                                                                          aaaGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAacac                                                                                                                                                                                         <  1:197220/185‑1 (MQ=255)                                                                                                          aaaGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAac                                                                                                                                                                                           <  1:129717/183‑1 (MQ=255)                                                                                                          aaaGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGa                                                                                                                       >  1:125621/1‑251 (MQ=255)                                                                                                          aaaGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGa                                                                                                                       >  1:31598/1‑251 (MQ=255)                                                                                                              agaCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAAt                                                                                                                     <  1:145628/249‑1 (MQ=255)                                                                                                              agaCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATa                                                                                                                    >  1:106675/1‑250 (MQ=255)                                                                                                               gaCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACgc                                                                                                                                                                                                                >  1:534264/1‑157 (MQ=255)                                                                                                               gaCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   >  1:343884/1‑250 (MQ=255)                                                                                                                aCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACgc                                                                                                                                                                                                                <  1:725479/156‑1 (MQ=255)                                                                                                                aCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   <  1:651705/249‑1 (MQ=255)                                                                                                                aCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   <  1:630515/249‑1 (MQ=255)                                                                                                                aCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   <  1:901389/249‑1 (MQ=255)                                                                                                                aCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   <  1:605018/249‑1 (MQ=255)                                                                                                                aCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   <  1:467086/249‑1 (MQ=255)                                                                                                                aCCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   <  1:506484/249‑1 (MQ=255)                                                                                                                       ccaaagccaaagATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGaaa                                                                                                          <  1:567936/251‑1 (MQ=255)                                                                                                                           agccaaagATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   <  1:843374/238‑1 (MQ=255)                                                                                                                            gccaaagATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCaaaaa                                                                                                                                                                                                        >  1:62097/1‑152 (MQ=255)                                                                                                                            gccaaagATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAac                                                                                                                                                                                           >  1:246854/1‑165 (MQ=255)                                                                                                                            gccaaagATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGaa                                                                                                                                                                   <  1:146333/189‑1 (MQ=255)                                                                                                                            gccaaagATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATAc                                                                                                                   <  1:696013/237‑1 (MQ=255)                                                                                                                                        aCCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGaa                                                                                         <  1:60573/251‑1 (MQ=255)                                                                                                                                             ggAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTgaga                                                                                                            <  1:646848/227‑1 (MQ=255)                                                                                                                                                  aaTTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGAc                                                                               <  1:136958/251‑1 (MQ=255)                                                                                                                                                        ccGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATc                                                                          <  1:215724/250‑1 (MQ=255)                                                                                                                                                              ggAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCacac                                                                                                                                                                                                                  >  1:549710/1‑108 (MQ=255)                                                                                                                                                               gAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGAc                                                                               >  1:726345/1‑238 (MQ=255)                                                                                                                                                                      gCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGt                                                             <  1:612382/249‑1 (MQ=255)                                                                                                                                                                           aaaaGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGgtg                                                      >  1:294213/1‑251 (MQ=255)                                                                                                                                                                            aaaGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAg                                                                                                                                                                                                      >  1:153491/1‑106 (MQ=255)                                                                                                                                                                               gACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCg                                                                                                                 >  1:360077/1‑188 (MQ=255)                                                                                                                                                                                   ccTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGgtgacgtgac                                               >  1:348041/1‑250 (MQ=255)                                                                                                                                                                                   ccTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGgtgacgtg                                                 <  1:357550/248‑1 (MQ=255)                                                                                                                                                                                         aaaaTGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGaa                                        <  1:650662/251‑1 (MQ=255)                                                                                                                                                                                                                   gaTAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAGTAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATcc                                                                         <  1:478353/192‑1 (MQ=255)                                                                                                                                                                                                                    aTAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGAAAAAGTCAAATCCCATGTGCGTTTTAAc             <  1:732636/251‑1 (MQ=255)                                                                                                                                                                                                                     tAAATCCACCGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGAAAAAGTCAAATCCCATGTGCGTTTTAACt            <  1:29881/251‑1 (MQ=255)                                                                                                                                                                                                                             ccGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTgaga                                                                                                            <  1:623104/147‑1 (MQ=255)                                                                                                                                                                                                                              cGCCAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGAAAAAGTCAAATCCCATGTGCGTTTTAACTAATCTTGAc   <  1:491574/251‑1 (MQ=255)                                                                                                                                                                                                                                ccAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGAAAAAGTCAAATCCCATGTGCGTTTTAACt            <  1:573299/240‑1 (MQ=255)                                                                                                                                                                                                                                ccAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGAAAAAGTCAAATCCCATGTGCGTTTTAACTAATCTTGACg  <  1:162063/250‑1 (MQ=255)                                                                                                                                                                                                                                ccAAAGAGAAAAGTGCGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGAAAAAGTCAAATCCCATGTGCGTTTTAACTAATCTTGACg  <  1:12797/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            ACCGTTAAATCCGATGCCAAAACCGTGGGCGGTAAAGTGAAAGAGGGCGCGGTGAAAGTCAAAGACGCGACCAAAGAGAAATTCGTTGAAGCGAAAGAGGCGACTAAAGAGACCTTTACCAAAGCCAAAGATGCTACCAAGGAAAAATTTGCCGAAGGGAAAAAGGCGGTAAAAGACACCTTTCAAAATGAAAAACCGGCGCCGGTAGAAGATAAATCCACCGCCAAAGAGAAAAGTACGGTGGAAAATAAAGCCAAAATCACACGCAGCAAAAAAGTGGATATTAACACTGCCGACGCAGCGACTTTAGAAAACCTATCCGGTATCGGTGAAGCAAAAGCTAAAGCAATTGTGGAATACCGTGAGAAAAACGGGAAATTTAAGAACCTGAATGACTTATCCAACGTGCCGGGTATCGGTGACGTGACCTTAGAAAAAGTCAAATCCCATGTGCGTTTTAACTAATCTTGACG  >  1/706352‑706824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑