Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	602,946	C→G	intergenic (+284/+133)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	602,946	0	C	G	100.0%	79.8 / NA	33	intergenic (+284/+133)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (12/21);  total (12/21)

AGGAGTAAGATTTAAAACGCCAGAGCAATCTAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAA  >  1/602699‑603021                                                                                                                                                                                                                                                        |                                                                            aagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGt                                                                          <  1:442944‑M2/221‑1 (MQ=255)       taaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAAtt                                                                    <  1:436222‑M2/227‑1 (MQ=255)                 gagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTTTGTTTATATGATGATTTTGGGTTAAATttgagggcgat                                                          >  1:421794‑M2/15‑247 (MQ=255)                      atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTtaaatttgagggcgattagtc                                                     <  1:681563‑M2/242‑10 (MQ=255)                      atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTtaaatttgagggcgattagtc                                                     <  1:824457‑M2/242‑10 (MQ=255)                      atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAAt                                                                     <  1:290052‑M2/226‑1 (MQ=255)                      atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCCTCGGTTTTGTTTTTGTTTTTATTATGGTTCTGCGGTtaattttagggcgattagt                                                      >  1:279353‑M2/10‑242 (MQ=255)                            cggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTctgcgttaaatttgagggcgattagtcgttatt                                               <  1:859078‑M2/248‑16 (MQ=255)                            cggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTctgcgttaaatttgagggcgattagtcgttatt                                               <  1:321418‑M2/248‑16 (MQ=255)                              gtggCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAgggcgattagtcgttattc                                              <  1:603904/247‑17 (MQ=25)                               tggCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAgggcgattagtcgttattca                                             <  1:767941/248‑18 (MQ=25)                                    gTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAgggcgattagtcgttatt                                               <  1:462008/243‑16 (MQ=37)                                    gTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGAGGGAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAgggcgattagtcgttatt                                               >  1:432932/1‑228 (MQ=25)                                              gCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAggg                                                              <  1:691838/218‑1 (MQ=255)                                              gCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTTAgggcgattagtcgttatt                                               >  1:647138/1‑218 (MQ=25)                                                cTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGt                                                                          <  1:13219/204‑1 (MQ=255)                                                cTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTTTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAg                           >  1:793873/1‑251 (MQ=25)                                                        cTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   <  1:126024/251‑5 (MQ=25)                                                        cTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   <  1:622206/251‑5 (MQ=25)                                                             tgtTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta              <  1:821071/251‑10 (MQ=25)                                                             tgtTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta              <  1:783339/251‑10 (MQ=25)                                                                   aGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTTTTTATGTTTATATGATGATTCTGCGTTAAATTTTAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAgtgt                        >  1:322279/1‑235 (MQ=21)                                                                   aGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTTAgggcgattagtcgttatt                                               >  1:348518/1‑197 (MQ=25)                                                                     caaacaaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaattattc      <  1:543917/251‑18 (MQ=21)                                                                      aaacaaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAACCGTCGCTTTTGTTTTTGTTTATATGATGATTCTGCGTTAAATTTGAgggcgatttgtcgttatt                                               >  1:649193/1‑194 (MQ=21)                                                                      aaacaaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCATCGTTTTTGTTTATGTTTATATGATGATTTTGCGTTAAATTTTAGGGGGGTTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   >  1:158898/1‑233 (MQ=21)                                                                         caaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaattattcatac  <  1:859192/251‑22 (MQ=21)                                                                                     gctgAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaatta         <  1:482745/232‑15 (MQ=21)                                                                                        gAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTTTGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   >  1:826309/1‑215 (MQ=21)                                                                                                           tGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgtt                    >  1:775368/1‑196 (MQ=25)                                                                                                                                    gCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta              >  1:440256/1‑171 (MQ=25)                                                                                                                                                       aaaTAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   <  1:258670/156‑5 (MQ=255)                                                                                                                                                         aTAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaat           <  1:284319/162‑13 (MQ=255)                                                                                                                                                                    gctCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaat           <  1:366774/149‑13 (MQ=255)                                                                                                                                                                                                                         tcgtTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   <  1:72476/90‑5 (MQ=255)                                                                                                                                                                                                                                                        |                                                                            AGGAGTAAGATTTAAAACGCCAGAGCAATCTAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAA  >  1/602699‑603021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑