Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	602,730	A→G	intergenic (+68/+349)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	602,730	0	A	G	100.0%	235.1 / NA	82	intergenic (+68/+349)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/44);  total (38/44)

CATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATC‑‑‑‑‑‑TAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGT  >  1/602494‑602976                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       cATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggtttt                                                                                                                                                                                                                                          >  1:355614/1‑242 (MQ=255)     aTCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                                                                      <  1:488391/251‑14 (MQ=255)         cTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTCCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTCATGATAGTTAAAAATACGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgcagc                                                                                                                                                                                                                                   >  1:222643/1‑234 (MQ=255)           gCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                                                                      >  1:240334/1‑232 (MQ=255)              gATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgcagcctgaaa                                                                                                                                                                                                                             >  1:278964/1‑229 (MQ=255)                   cGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                                                                      <  1:309368/237‑14 (MQ=255)                     cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTg                                                                                                                                                                                                                              >  1:144640/1‑249 (MQ=255)                     cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAg                                                                                                                                                                                                                            >  1:366439/1‑251 (MQ=255)                              cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                                                                    <  1:238959/250‑1 (MQ=255)                              cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                                                                    <  1:547566/250‑1 (MQ=255)                                    aagaaAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                                                                      >  1:537335/1‑207 (MQ=255)                                        aaGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAg                                                                                                                                                                                                           >  1:811874/1‑249 (MQ=255)                                             ggtatgatgactattcgagagaatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTaa                                                                                                                                                                                                      >  1:111492‑M2/198‑249 (MQ=255)                                                       ctattcgagagaatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAag                                                                                                                                                                                          >  1:464298‑M2/188‑251 (MQ=255)                                                        tattcgagagaatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatgtatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAagc                                                                                                                                                                                         <  1:450445‑M2/65‑1 (MQ=255)                                                         attcgagagaatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAagca                                                                                                                                                                                        >  1:557638‑M2/186‑251 (MQ=255)                                                         attcgagagaatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAagca                                                                                                                                                                                        >  1:869732‑M2/186‑251 (MQ=255)                                                                agaatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAg                                                                                                                                                                                  <  1:15288‑M2/72‑1 (MQ=255)                                                                 gaatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAActgc                                                                                                                                                                                                  >  1:746604‑M2/178‑233 (MQ=255)                                                                 gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGcaaacaa                                                                                                                                                                                                             <  1:136009/222‑1 (MQ=255)                                                                  aatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGaaa                                                                                                                                                                               <  1:830664‑M2/75‑1 (MQ=255)                                                                  aatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGaaa                                                                                                                                                                               <  1:793022‑M2/75‑1 (MQ=255)                                                                  aatgtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGaaa                                                                                                                                                                               >  1:709535‑M2/177‑251 (MQ=255)                                                                     gtgcagacgcacacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAag                                                                                                                                                                                          <  1:498614‑M2/64‑1 (MQ=255)                                                                       aagagacatcacttggattttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGt                                                                                                                                                                          <  1:412469‑M2/80‑1 (MQ=255)                                                                                cacTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGc                                                                                                                                                                                                          >  1:571215/1‑210 (MQ=255)                                                                                       attttctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagatgaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACGGAAAg                                                                                                                                                           <  1:448613‑M2/95‑1 (MQ=255)                                                                                            ctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGaaa                                                                                                                                                                               >  1:368206‑M2/151‑225 (MQ=255)                                                                                            ctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCa                                                                                                                                                       >  1:652735‑M2/151‑249 (MQ=255)                                                                                            ctacaagatgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTAAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAagc                                                                                                                                                                                         >  1:453487‑M2/151‑215 (MQ=255)                                                                                                   atgcgggagctaaatttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAAc                                                                                                                                              <  1:729806‑M2/108‑1 (MQ=255)                                                                                                                atttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAActgctg                                                                                                                                                                                                <  1:476758‑M2/58‑1 (MQ=255)                                                                                                                atttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGctc                                                                                                                                                                     >  1:84616‑M2/131‑215 (MQ=255)                                                                                                                atttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGaa                                                                                                                                 >  1:367057‑M2/131‑251 (MQ=255)                                                                                                                atttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGaa                                                                                                                                 >  1:74929‑M2/131‑251 (MQ=255)                                                                                                                atttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGaa                                                                                                                                 >  1:513870‑M2/131‑251 (MQ=255)                                                                                                                atttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGaa                                                                                                                                 >  1:28088‑M2/131‑251 (MQ=255)                                                                                                                aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                                                                    <  1:816764/168‑1 (MQ=255)                                                                                                                 tttaatagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGaaa                                                                                                                                <  1:730728‑M2/122‑1 (MQ=255)                                                                                                                     atagagatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAAtag                                                                                                                            <  1:720344‑M2/126‑1 (MQ=255)                                                                                                                         agatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATaa                                                                                                                                                                                           >  1:182497‑M2/122‑184 (MQ=255)                                                                                                                          gatgaggcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAAc                                                                                                                                              >  1:419665‑M2/121‑228 (MQ=255)                                                                                                                                gcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAAt                                                                                                                              >  1:251016‑M2/115‑238 (MQ=255)                                                                                                                                gcattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTaagct                                                                                                                  <  1:379077‑M2/136‑3 (MQ=255)                                                                                                                                 cattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGaa                                                                                                                                                             <  1:115508‑M2/93‑1 (MQ=255)                                                                                                                                 cattagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTtaagctc                                                                                                                 <  1:156422‑M2/137‑4 (MQ=255)                                                                                                                                   ttagatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTAc                                                                                                              <  1:168102‑M2/140‑1 (MQ=255)                                                                                                                                     agatttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACt                                                                                                             >  1:348389‑M2/110‑250 (MQ=255)                                                                                                                                       atttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTActc                                                                                                            <  1:858124‑M2/142‑2 (MQ=255)                                                                                                                                       atttatttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTActc                                                                                                            <  1:590268‑M2/142‑2 (MQ=255)                                                                                                                                           atttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCAc                                                                                                      <  1:23606‑M2/148‑1 (MQ=255)                                                                                                                                                    agctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGc                                                                                                                                                   >  1:719171‑M2/95‑197 (MQ=255)                                                                                                                                                    agctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAagc                                                                                                                   >  1:24663‑M2/95‑228 (MQ=255)                                                                                                                                                      ctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAg                                                                                            <  1:802821‑M2/158‑1 (MQ=255)                                                                                                                                                             ggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAAc                                                                                                                                              >  1:137816‑M2/86‑193 (MQ=255)                                                                                                                                                                 aaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAAc                                                                                <  1:21087‑M2/170‑1 (MQ=255)                                                                                                                                                                   agattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGc                                                                                                                                       >  1:234151‑M2/80‑194 (MQ=255)                                                                                                                                                                    gattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAAcgg                                                                              >  1:489507‑M2/79‑250 (MQ=255)                                                                                                                                                                    gattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCCGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAacggg                                                                             >  1:788641‑M2/79‑250 (MQ=255)                                                                                                                                                                                ttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAcc                                                                  <  1:505072‑M2/184‑1 (MQ=255)                                                                                                                                                                                ttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAc                                                                   <  1:460813‑M2/183‑1 (MQ=255)                                                                                                                                                                                    aaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGATCAGGCAGACAAAAACGGCGGGGTAATACCcgtcg                                                             <  1:388202‑M2/189‑1 (MQ=255)                                                                                                                                                                                     aataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGaaa                                                                                                                                                                               >  1:292646‑M2/62‑136 (MQ=255)                                                                                                                                                                                                  ccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTtgtttatgtt                                               <  1:823710‑M2/203‑1 (MQ=255)                                                                                                                                                                                                  ccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTtgtttatgtt                                               <  1:662066‑M2/203‑1 (MQ=255)                                                                                                                                                                                                      caattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTtata                                           <  1:639172‑M2/207‑1 (MQ=255)                                                                                                                                                                                                       aattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTatat                                          <  1:295208‑M2/208‑1 (MQ=255)                                                                                                                                                                                                               ggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGaaa                                                                                                                                <  1:383722‑M2/122‑1 (MQ=255)                                                                                                                                                                                                               ggattaagatgtaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAAtagtag                                                                                                                         <  1:518242‑M2/129‑1 (MQ=255)                                                                                                                                                                                                                gattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATtc                                  <  1:179525‑M2/216‑1 (MQ=255)                                                                                                                                                                                                                gattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTTTTTATGTTTATATGATGAtt                                   >  1:716652‑M2/35‑249 (MQ=255)                                                                                                                                                                                                                    aagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGt                             <  1:442944‑M2/221‑1 (MQ=255)                                                                                                                                                                                                                      gatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTtgtttatg                                                 <  1:224828‑M2/201‑1 (MQ=255)                                                                                                                                                                                                                          taaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAAtt                       <  1:436222‑M2/227‑1 (MQ=255)                                                                                                                                                                                                                               tgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTg                                                                                                                                                               >  1:507466‑M2/20‑110 (MQ=255)                                                                                                                                                                                                                                    gagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTTTGTTTATATGATGATTTTGGGTTAAATttgagggcgat             >  1:421794‑M2/15‑247 (MQ=255)                                                                                                                                                                                                                                         atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTtaaatttgagggcgattagtc        <  1:681563‑M2/242‑10 (MQ=255)                                                                                                                                                                                                                                         atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTtaaatttgagggcgattagtc        <  1:824457‑M2/242‑10 (MQ=255)                                                                                                                                                                                                                                         atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAAt                        <  1:290052‑M2/226‑1 (MQ=255)                                                                                                                                                                                                                                         atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCCTCGGTTTTGTTTTTGTTTTTATTATGGTTCTGCGGTtaattttagggcgattagt         >  1:279353‑M2/10‑242 (MQ=255)                                                                                                                                                                                                                                               cggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTctgcgttaaatttgagggcgattagtcgttatt  <  1:321418‑M2/248‑16 (MQ=255)                                                                                                                                                                                                                                               cggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTctgcgttaaatttgagggcgattagtcgttatt  <  1:859078‑M2/248‑16 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       CATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATC‑‑‑‑‑‑TAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGT  >  1/602494‑602976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑