Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	602,716	C→T	intergenic (+54/+363)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	602,716	0	C	T	100.0%	222.3 / NA	78	intergenic (+54/+363)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/41);  total (37/41)

ATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATC‑‑‑‑‑‑TAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATT  >  1/602481‑602955                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      atgaCATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtgg                                                                                                                                                                                                                                        >  1:260116‑M1/1‑248 (MQ=255)              cATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggtttt                                                                                                                                                                                                                     >  1:355614/1‑242 (MQ=255)                  aTCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                                                 <  1:488391/251‑14 (MQ=255)                   tCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGc                                                                                                                                                                                                                                               <  1:645136/226‑1 (MQ=255)                      cTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTCCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTCATGATAGTTAAAAATACGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgcagc                                                                                                                                                                                                              >  1:222643/1‑234 (MQ=255)                        gCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                                                 >  1:240334/1‑232 (MQ=255)                           gATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgcagcctgaaa                                                                                                                                                                                                        >  1:278964/1‑229 (MQ=255)                                cGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                                                 <  1:309368/237‑14 (MQ=255)                                  cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTg                                                                                                                                                                                                         >  1:144640/1‑249 (MQ=255)                                  cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAg                                                                                                                                                                                                       >  1:366439/1‑251 (MQ=255)                                           cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                                               <  1:547566/250‑1 (MQ=255)                                           cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                                               <  1:238959/250‑1 (MQ=255)                                                 aagaaAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                                                 >  1:537335/1‑207 (MQ=255)                                                     aaGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAg                                                                                                                                                                                      >  1:811874/1‑249 (MQ=255)                                                          ggTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaa                                                                                                                                                                                 >  1:111492‑M1/1‑191 (MQ=255)                                                            tATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCt                                                                                                                                                                                                                                          <  1:77592‑M1/190‑2 (MQ=255)                                                                    cTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataag                                                                                                                                                                     >  1:464298‑M1/1‑181 (MQ=255)                                                                     tATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATGTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagc                                                                                                                                                                    <  1:450445‑M1/251‑72 (MQ=255)                                                                      aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagca                                                                                                                                                                   >  1:869732‑M1/1‑179 (MQ=255)                                                                      aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagca                                                                                                                                                                   >  1:557638‑M1/1‑179 (MQ=255)                                                                             agaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttag                                                                                                                                                             <  1:15288‑M1/250‑79 (MQ=255)                                                                              gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgc                                                                                                                                                                             >  1:746604‑M1/1‑171 (MQ=255)                                                                              gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGcaaacaa                                                                                                                                                                                        <  1:136009/222‑1 (MQ=255)                                                                               aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                                          >  1:709535‑M1/1‑170 (MQ=255)                                                                               aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                                          <  1:793022‑M1/251‑82 (MQ=255)                                                                               aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                                          <  1:830664‑M1/251‑82 (MQ=255)                                                                                  gtgCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataag                                                                                                                                                                     <  1:498614‑M1/237‑71 (MQ=255)                                                                                    aagagacatcacttggattttCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggt                                                                                                                                                     <  1:412469‑M1/242‑87 (MQ=255)                                                                                             cacTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGc                                                                                                                                                                                     >  1:571215/1‑210 (MQ=255)                                                                                                    aTTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATGAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttacggaaag                                                                                                                                      <  1:448613‑M1/250‑102 (MQ=255)                                                                                                         ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttca                                                                                                                                  >  1:652735‑M1/2‑144 (MQ=255)                                                                                                         ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                                          >  1:368206‑M1/2‑144 (MQ=255)                                                                                                         ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctaaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagc                                                                                                                                                                    >  1:453487‑M1/2‑144 (MQ=255)                                                                                                                aTGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                                                         <  1:729806‑M1/251‑115 (MQ=255)                                                                                                                           aaaTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCagag                                                                                                                                                                                                                                                >  1:484286/1‑121 (MQ=255)                                                                                                                             aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                                                            >  1:28088‑M1/1‑124 (MQ=255)                                                                                                                             aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                                                            >  1:513870‑M1/1‑124 (MQ=255)                                                                                                                             aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                                                            >  1:367057‑M1/1‑124 (MQ=255)                                                                                                                             aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                                                            >  1:74929‑M1/1‑124 (MQ=255)                                                                                                                             aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctc                                                                                                                                                >  1:84616‑M1/1‑124 (MQ=255)                                                                                                                             aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctg                                                                                                                                                                           <  1:476758‑M1/188‑65 (MQ=255)                                                                                                                             aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                                               <  1:816764/168‑1 (MQ=255)                                                                                                                              tttAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaa                                                                                                           <  1:730728‑M1/251‑129 (MQ=255)                                                                                                                                  aTAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatag                                                                                                       <  1:720344‑M1/251‑133 (MQ=255)                                                                                                                                      agaTGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataa                                                                                                                                                                      >  1:182497‑M1/1‑115 (MQ=255)                                                                                                                                       gaTGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                                                         >  1:419665‑M1/1‑114 (MQ=255)                                                                                                                                             gcattagaTTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagct                                                                                             <  1:379077‑M1/247‑143 (MQ=255)                                                                                                                                             gcattagaTTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaat                                                                                                         >  1:251016‑M1/4‑108 (MQ=255)                                                                                                                                              cattagATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctc                                                                                            <  1:156422‑M1/248‑144 (MQ=255)                                                                                                                                              cattagATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaa                                                                                                                                        <  1:115508‑M1/204‑100 (MQ=255)                                                                                                                                                ttAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctac                                                                                         <  1:168102‑M1/251‑147 (MQ=255)                                                                                                                                                  aGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctact                                                                                        >  1:348389‑M1/1‑103 (MQ=255)                                                                                                                                                    atttatttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactc                                                                                       <  1:590268‑M1/249‑149 (MQ=255)                                                                                                                                                    atttatttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactc                                                                                       <  1:858124‑M1/249‑149 (MQ=255)                                                                                                                                                        atttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcac                                                                                 <  1:23606‑M1/251‑155 (MQ=255)                                                                                                                                                                 aGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagc                                                                                              >  1:24663‑M1/1‑88 (MQ=255)                                                                                                                                                                 aGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgc                                                                                                                              >  1:719171‑M1/1‑88 (MQ=255)                                                                                                                                                                   cTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacag                                                                       <  1:802821‑M1/250‑165 (MQ=255)                                                                                                                                                                          ggAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                                                         >  1:137816‑M1/1‑79 (MQ=255)                                                                                                                                                                              aaaGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaac                                                           <  1:21087‑M1/251‑177 (MQ=255)                                                                                                                                                                                aGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagc                                                                                                                  >  1:234151‑M1/1‑73 (MQ=255)                                                                                                                                                                                 gATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacgg                                                         >  1:489507‑M1/1‑72 (MQ=255)                                                                                                                                                                                 gATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggccgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggg                                                        >  1:788641‑M1/1‑72 (MQ=255)                                                                                                                                                                                             ttAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacc                                             <  1:505072‑M1/250‑191 (MQ=255)                                                                                                                                                                                             ttAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatac                                              <  1:460813‑M1/249‑190 (MQ=255)                                                                                                                                                                                                 aaaTAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgatcaggcagacaaaaacggcggggtaatacccgtcg                                        <  1:388202‑M1/251‑196 (MQ=255)                                                                                                                                                                                                  aaTAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                                          >  1:292646‑M1/1‑55 (MQ=255)                                                                                                                                                                                                               ccGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtt                          <  1:662066‑M1/251‑210 (MQ=255)                                                                                                                                                                                                               ccGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtt                          <  1:823710‑M1/251‑210 (MQ=255)                                                                                                                                                                                                                   cAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtttata                      <  1:639172‑M1/251‑214 (MQ=255)                                                                                                                                                                                                                    aaTTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtttatat                     <  1:295208‑M1/251‑215 (MQ=255)                                                                                                                                                                                                                            ggattaagaTTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaa                                                                                                           <  1:383722‑M1/153‑129 (MQ=255)                                                                                                                                                                                                                            ggattaagaTGTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtag                                                                                                    <  1:518242‑M1/160‑136 (MQ=255)                                                                                                                                                                                                                             gattaagATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtttatatgatgattc             <  1:179525‑M1/247‑223 (MQ=255)                                                                                                                                                                                                                             gattaagATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcgggggaatacccgtcgtttttttttatgtttatatgatgatt              >  1:716652‑M1/4‑28 (MQ=255)                                                                                                                                                                                                                                 aaGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtttatatgatgattctgcgt        <  1:442944‑M1/251‑228 (MQ=255)                                                                                                                                                                                                                                   gATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatg                            <  1:224828‑M1/229‑208 (MQ=255)                                                                                                                                                                                                                                       tAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtttatatgatgattctgcgttaaatt  <  1:436222‑M1/251‑234 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      ATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATC‑‑‑‑‑‑TAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATT  >  1/602481‑602955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑