Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	602,703	G→T	intergenic (+41/+376)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	602,703	0	G	T	100.0%	216.2 / NA	76	intergenic (+41/+376)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (40/36);  total (40/36)

GCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATC‑‑‑‑‑‑TAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATAT  >  1/602459‑602936                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGAt                                                                                                                                                                                                                                           >  1:722171/1‑251 (MQ=255)  cTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGa                                                                                                                                                                                                                                            <  1:761686/249‑1 (MQ=255)        aTGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaat                                                                                                                                                                                                                                    >  1:292006/1‑250 (MQ=255)                 atCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGCAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaaatgccagag                                                                                                                                                                                                                            >  1:47801/1‑241 (MQ=255)                       atgaCATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtgg                                                                                                                                                                                                                     >  1:260116‑M1/1‑248 (MQ=255)                                    cATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaa                                                                                                                                                                                                                                     >  1:743201/1‑222 (MQ=255)                                    cATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggtttt                                                                                                                                                                                                  >  1:355614/1‑242 (MQ=255)                                        aTCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                              <  1:488391/251‑14 (MQ=255)                                         tCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGc                                                                                                                                                                                                                            <  1:645136/226‑1 (MQ=255)                                            cTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTCCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTCATGATAGTTAAAAATACGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgcagc                                                                                                                                                                                           >  1:222643/1‑234 (MQ=255)                                              gCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                              >  1:240334/1‑232 (MQ=255)                                                 gATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgcagcctgaaa                                                                                                                                                                                     >  1:278964/1‑229 (MQ=255)                                                      cGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                              <  1:309368/237‑14 (MQ=255)                                                        cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTg                                                                                                                                                                                      >  1:144640/1‑249 (MQ=255)                                                        cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAg                                                                                                                                                                                    >  1:366439/1‑251 (MQ=255)                                                                 cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                            <  1:547566/250‑1 (MQ=255)                                                                 cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                            <  1:238959/250‑1 (MQ=255)                                                                       aagaaAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATC‑‑‑‑‑‑TGGCGGtggcggttttatgc                                                                                                                                                                                              >  1:537335/1‑207 (MQ=255)                                                                           aaGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAg                                                                                                                                                                   >  1:811874/1‑249 (MQ=255)                                                                                ggTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaa                                                                                                                                                              >  1:111492‑M1/1‑191 (MQ=255)                                                                                  tATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCt                                                                                                                                                                                                                       <  1:77592‑M1/190‑2 (MQ=255)                                                                                          cTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataag                                                                                                                                                  >  1:464298‑M1/1‑181 (MQ=255)                                                                                           tATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATGTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagc                                                                                                                                                 <  1:450445‑M1/251‑72 (MQ=255)                                                                                            aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagca                                                                                                                                                >  1:557638‑M1/1‑179 (MQ=255)                                                                                            aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagca                                                                                                                                                >  1:869732‑M1/1‑179 (MQ=255)                                                                                                   agaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttag                                                                                                                                          <  1:15288‑M1/250‑79 (MQ=255)                                                                                                    gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgc                                                                                                                                                          >  1:746604‑M1/1‑171 (MQ=255)                                                                                                    gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGcaaacaa                                                                                                                                                                     <  1:136009/222‑1 (MQ=255)                                                                                                     aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                       >  1:709535‑M1/1‑170 (MQ=255)                                                                                                     aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                       <  1:793022‑M1/251‑82 (MQ=255)                                                                                                     aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                       <  1:830664‑M1/251‑82 (MQ=255)                                                                                                        gtgCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataag                                                                                                                                                  <  1:498614‑M1/237‑71 (MQ=255)                                                                                                          aagagacatcacttggattttCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggt                                                                                                                                  <  1:412469‑M1/242‑87 (MQ=255)                                                                                                                   cacTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGc                                                                                                                                                                  >  1:571215/1‑210 (MQ=255)                                                                                                                          aTTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATGAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttacggaaag                                                                                                                   <  1:448613‑M1/250‑102 (MQ=255)                                                                                                                               ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttca                                                                                                               >  1:652735‑M1/2‑144 (MQ=255)                                                                                                                               ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                       >  1:368206‑M1/2‑144 (MQ=255)                                                                                                                               ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctaaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagc                                                                                                                                                 >  1:453487‑M1/2‑144 (MQ=255)                                                                                                                                      aTGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                                      <  1:729806‑M1/251‑115 (MQ=255)                                                                                                                                                 aaaTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCagag                                                                                                                                                                                                                             >  1:484286/1‑121 (MQ=255)                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                                         >  1:74929‑M1/1‑124 (MQ=255)                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                                         >  1:28088‑M1/1‑124 (MQ=255)                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                                         >  1:367057‑M1/1‑124 (MQ=255)                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                                         >  1:513870‑M1/1‑124 (MQ=255)                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctc                                                                                                                             >  1:84616‑M1/1‑124 (MQ=255)                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctg                                                                                                                                                        <  1:476758‑M1/188‑65 (MQ=255)                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCTGGCGGTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAg                                                                                                                                                                            <  1:816764/168‑1 (MQ=255)                                                                                                                                                    tttAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaa                                                                                        <  1:730728‑M1/251‑129 (MQ=255)                                                                                                                                                        aTAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatag                                                                                    <  1:720344‑M1/251‑133 (MQ=255)                                                                                                                                                            agaTGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataa                                                                                                                                                   >  1:182497‑M1/1‑115 (MQ=255)                                                                                                                                                             gaTGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                                      >  1:419665‑M1/1‑114 (MQ=255)                                                                                                                                                                   gcattagaTTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagct                                                                          <  1:379077‑M1/247‑143 (MQ=255)                                                                                                                                                                   gcattagaTTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaat                                                                                      >  1:251016‑M1/4‑108 (MQ=255)                                                                                                                                                                    cattagATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctc                                                                         <  1:156422‑M1/248‑144 (MQ=255)                                                                                                                                                                    cattagATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaa                                                                                                                     <  1:115508‑M1/204‑100 (MQ=255)                                                                                                                                                                      ttAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctac                                                                      <  1:168102‑M1/251‑147 (MQ=255)                                                                                                                                                                        aGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctact                                                                     >  1:348389‑M1/1‑103 (MQ=255)                                                                                                                                                                          atttatttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactc                                                                    <  1:858124‑M1/249‑149 (MQ=255)                                                                                                                                                                          atttatttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactc                                                                    <  1:590268‑M1/249‑149 (MQ=255)                                                                                                                                                                              atttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcac                                                              <  1:23606‑M1/251‑155 (MQ=255)                                                                                                                                                                                       aGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagc                                                                           >  1:24663‑M1/1‑88 (MQ=255)                                                                                                                                                                                       aGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgc                                                                                                           >  1:719171‑M1/1‑88 (MQ=255)                                                                                                                                                                                         cTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacag                                                    <  1:802821‑M1/250‑165 (MQ=255)                                                                                                                                                                                                ggAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                                      >  1:137816‑M1/1‑79 (MQ=255)                                                                                                                                                                                                    aaaGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaac                                        <  1:21087‑M1/251‑177 (MQ=255)                                                                                                                                                                                                      aGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagc                                                                                               >  1:234151‑M1/1‑73 (MQ=255)                                                                                                                                                                                                       gATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacgg                                      >  1:489507‑M1/1‑72 (MQ=255)                                                                                                                                                                                                       gATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggccgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggg                                     >  1:788641‑M1/1‑72 (MQ=255)                                                                                                                                                                                                                   ttAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacc                          <  1:505072‑M1/250‑191 (MQ=255)                                                                                                                                                                                                                   ttAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatac                           <  1:460813‑M1/249‑190 (MQ=255)                                                                                                                                                                                                                       aaaTAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgatcaggcagacaaaaacggcggggtaatacccgtcg                     <  1:388202‑M1/251‑196 (MQ=255)                                                                                                                                                                                                                        aaTAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                                       >  1:292646‑M1/1‑55 (MQ=255)                                                                                                                                                                                                                                     ccGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtt       <  1:823710‑M1/251‑210 (MQ=255)                                                                                                                                                                                                                                     ccGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtt       <  1:662066‑M1/251‑210 (MQ=255)                                                                                                                                                                                                                                         cAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtttata   <  1:639172‑M1/251‑214 (MQ=255)                                                                                                                                                                                                                                          aaTTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacccgtcgtttttgtttatgtttatat  <  1:295208‑M1/251‑215 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATC‑‑‑‑‑‑TAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATAT  >  1/602459‑602936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑