Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 91,485 G→A intergenic (‑72/+95) unknown ← / ← unknown hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*191,4850GA100.0% 79.0 / NA 29intergenic (‑72/+95)unknown/unknownhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/15);  total (14/15)

TTGCTTCTAACCAGCCATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTACCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATGTTATATTATATAATATAACCGCGCAACCGCTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTAATCGCTATCCGCTTAGCATTGTCAAACGCTTGCCAGTTATCTACTTTATCCGTTTGTATATACTCAATAAAGCGCACAAACTCGCTTTTGG  >  1/91241‑91728
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
ttGCTTCTAACCAGCCATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATAttatat                                                                                                                                                                                                                                               <  1:232394/251‑1 (MQ=255)
   cTTCTAACCAGCCATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAAtattatatta                                                                                                                                                                                                                                             <  1:447360/250‑1 (MQ=255)
          ccAGCCATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTatataatat                                                                                                                                                                                                                                     <  1:454660/251‑1 (MQ=255)
          ccAGCCATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTatataatat                                                                                                                                                                                                                                     <  1:283285/251‑1 (MQ=255)
              ccATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTatataatat                                                                                                                                                                                                                                     >  1:520133/1‑247 (MQ=255)
              ccATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAAc                                                                                                                                                                                                                                  <  1:60703/250‑1 (MQ=255)
               cATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTAAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAActg                                                                                                                                                                                                                                <  1:696227/251‑3 (MQ=255)
                 ttCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAAc                                                                                                                                                                                                                                  >  1:506796/1‑247 (MQ=255)
                      gcgcATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATAttatat                                                                                                                                                                                                                                               >  1:182467/1‑229 (MQ=255)
                      gcgcATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAAc                                                                                                                                                                                                                          >  1:610117/1‑250 (MQ=255)
                             gCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTatataatat                                                                                                                                                                                                                                     >  1:792733/1‑232 (MQ=255)
                                                   gggATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATAttt                                                                                                                                                                                            >  1:741810/1‑251 (MQ=255)
                                                      aTCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATtatattatat                                                                                                                                                                                                                                          <  1:156552/202‑1 (MQ=255)
                                                             cATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATAttatat                                                                                                                                                                                                                                               >  1:606949/1‑190 (MQ=255)
                                                                             ttcGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAg                                                                                                                                                                  <  1:52956/251‑1 (MQ=255)
                                                                               cGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAggg                                                                                                                                                                <  1:899258/251‑1 (MQ=255)
                                                                                           gCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTatataatat                                                                                                                                                                                                                                     <  1:299404/170‑1 (MQ=255)
                                                                                                    ttGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAggg                                                                                                                                                                <  1:879623/230‑1 (MQ=255)
                                                                                                                      cTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAata                                                                                                                                                                                               >  1:666076/1‑181 (MQ=255)
                                                                                                                                   tttttttGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAg                                                                                                            <  1:565840/251‑1 (MQ=255)
                                                                                                                                          ggTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATAc                                                                                                                        >  1:375626/1‑232 (MQ=255)
                                                                                                                                                   aTGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTGCCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTaa                                                                                            >  1:327121/1‑251 (MQ=255)
                                                                                                                                                                                                cGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTAATc                                                                                          <  1:662032/208‑1 (MQ=255)
                                                                                                                                                                                                         ttGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATAttatat                                                                                                                                                                                                                                               >  1:276575/1‑50 (MQ=255)
                                                                                                                                                                                                         ttGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATa                                                                                                                         <  1:251835/168‑1 (MQ=255)
                                                                                                                                                                                                         ttGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTAATCGCTATCCGc                                                                                 <  1:68252/208‑1 (MQ=255)
                                                                                                                                                                                                                             tCCCTCTTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTAATCGCTATCCGCTTAGCATTaacgcttgccagttatctactttatccgtttgtatatactcaataaagcgcacaa                  >  1:519533‑M1/1‑196 (MQ=255)
                                                                                                                                                                                                                                  ctTGCTTACGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTAATCGCTATCCGCTTAGCATTaacgcttgccagttatctactttatccgtttgtatatactcaataaagcgcacaaactcg             >  1:807424‑M1/1‑191 (MQ=255)
                                                                                                                                                                                                                                          cGTGTCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTAATCGCTATCCGCTTAGCATTaacgcttgccagttatctactttatccgtttgtatatactcaataaagcgcacaaactcgctc          <  1:218500‑M1/246‑64 (MQ=255)
                                                                                                                                                                                                                                             gtCTAATATTATATTATATAATATAACTGCGCAACCGGTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTAATCGCTATCCGCTTAGCATTaacgcttgccagttatctactttatccgtttgtatatactcaataaagcgcacaaactcgctcttagttga  >  1:248287‑M1/1‑180 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
TTGCTTCTAACCAGCCATTCCGGCGCATAGCCGTTTTTCATTTGAGATTTCGGGATCCAAACATTTACTTTTTTCTTTTCGCCATCAAACAGCTCAACGTTTGCAATAAACATCATTGCTTTTTCACTTTCTTTTTTTGGTTTTCCGATGTTGATGTATTTTTGAGTTGTCATTTTTGTATCTCCTACCGCTCGTTAATCTTTGTAAGCCTCGGTGCGGCGTCCCTCTTGCTTACGTGTCTAATGTTATATTATATAATATAACCGCGCAACCGCTATTTTGTAAGTTTTGTGTAAATATTTAAAAGCCCCTTAAACATCGTTTAAAGGGCTTTTGAAGTTAAAATTTATAAACCAGATTATCCTCATACTCGCCATGATAGTTGAGGTTTGTCTTAATCGCTATCCGCTTAGCATTGTCAAACGCTTGCCAGTTATCTACTTTATCCGTTTGTATATACTCAATAAAGCGCACAAACTCGCTTTTGG  >  1/91241‑91728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑