BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	532,190	T→C	C113R (TGT→CGT)	unknown →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	532,190	0	T	C	100.0%	400.7 / NA	140	C113R (TGT→CGT)	unknown	O‑antigen ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (52/88); total (52/88)

Only 100 of 140 total aligned reads displayed.

TAACTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATATGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTCAGTCTGATGGGCGTGTTCGC  >  1/531943‑532431

taACTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCGAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGtt                                                                                                                                                                                                                                                >  1:440426/1‑251 (MQ=255)

taACTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGtt                                                                                                                                                                                                                                                >  1:374260/1‑251 (MQ=255)

taACTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGtt                                                                                                                                                                                                                                                >  1:505121/1‑251 (MQ=255)

taACTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGtt                                                                                                                                                                                                                                                >  1:296447/1‑251 (MQ=255)

taACTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGtt                                                                                                                                                                                                                                                <  1:533865/251‑1 (MQ=255)

 aaCTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCACAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGttt                                                                                                                                                                                                                                               >  1:447082/1‑251 (MQ=255)

  aCTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGtttt                                                                                                                                                                                                                                              >  1:382099/1‑251 (MQ=255)

     ataCCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAAt                                                                                                                                                                                                                                           <  1:71254/251‑1 (MQ=255)

      taCCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAAtt                                                                                                                                                                                                                                          <  1:46607/251‑1 (MQ=255)

      taCCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTAGGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGATACTGGCGCATTATCCGATACGTTTTAAtt                                                                                                                                                                                                                                          <  1:423783/251‑1 (MQ=255)

               aaTCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTac                                                                                                                                                                                                                                 <  1:420953/251‑1 (MQ=255)

               aaTCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTac                                                                                                                                                                                                                                 <  1:4335/251‑1 (MQ=255)

                aTCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTaca                                                                                                                                                                                                                                >  1:849892/1‑251 (MQ=255)

                     gTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCa                                                                                                                                                                                                                           >  1:32365/1‑251 (MQ=255)

                      tCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCAt                                                                                                                                                                                                                          >  1:144521/1‑251 (MQ=255)

                            gtGGGCGTAATTTTTTGCATTTGCCGCCGATTATGCAAACCGAAGGGCAATCTCTCCTATGATGGCAATGAGAAATGGTTTGTTGCCCCCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTACGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGAATTCTCTTTTTTCTAACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCAt                                                                                                                                                                                                                     <  1:493900/249‑1 (MQ=255)

                            aTGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCAt                                                                                                                                                                                                                     <  1:690681/250‑1 (MQ=255)

                            aTGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCAt                                                                                                                                                                                                                     <  1:624480/250‑1 (MQ=255)

                            aTGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCAt                                                                                                                                                                                                                     <  1:529013/250‑1 (MQ=255)

                                 cGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGc                                                                                                                                                                                                                <  1:851877/250‑1 (MQ=255)

                                    aaTTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGcc                                                                                                                                                                                                            <  1:463141/251‑1 (MQ=255)

                                        tttttCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGAt                                                                                                                                                                                                        <  1:88620/251‑1 (MQ=255)

                                          ttacATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCGCCCTGCTCTTTTACGTGCTGCTCTTGGCCCTTGCGATTCTGTACCATCAGGGCAAAATTCGGGAAGTTGATACCCCGAGCCGCATTCTCTTTTTTCTGGCCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATGGGGTTTTACACGCCATTCCATGGGGCAGCCTGATTg                                                                                                                                                                                                      <  1:870628/248‑1 (MQ=255)

                                               tttACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGc                                                                                                                                                                                                 <  1:653815/251‑1 (MQ=255)

                                                ttACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGcc                                                                                                                                                                                                                            >  1:693181/1‑223 (MQ=255)

                                                  aCCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCAt                                                                                                                                                                                               >  1:529293/1‑250 (MQ=255)

                                                      ccAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCAt                                                                                                                                                                                                                     <  1:31318/224‑1 (MQ=255)

                                                          ttATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTGGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCtat                                                                                                                                                                                      <  1:743359/251‑1 (MQ=255)

                                                            aTGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCtatta                                                                                                                                                                                    <  1:662845/251‑1 (MQ=255)

                                                               cAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTattatt                                                                                                                                                                                  <  1:588945/250‑1 (MQ=255)

                                                               cAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTc                                                                                                                                                                                 <  1:375811/251‑1 (MQ=255)

                                                               cAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTc                                                                                                                                                                                 <  1:898638/251‑1 (MQ=255)

                                                               cAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTc                                                                                                                                                                                 <  1:782842/251‑1 (MQ=255)

                                                               cAAACCGAAGGGCAATCTCACCTAGGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTc                                                                                                                                                                                 <  1:713946/251‑1 (MQ=255)

                                                                 aaCCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCAt                                                                                                                                                                               <  1:377556/251‑1 (MQ=255)

                                                                   ccGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGcc                                                                                                                                                                                                                            >  1:22849/1‑204 (MQ=255)

                                                                        gggCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTa                                                                                                                                                                        >  1:695444/1‑251 (MQ=255)

                                                                        gggCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTa                                                                                                                                                                        >  1:8703/1‑251 (MQ=255)

                                                                           cAATCTCCCCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCTTTACAGGCGCATTATCCGATCCGTTTTAATTGGCTTTTAAACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCt                                                                                                                                                                     <  1:77635/251‑1 (MQ=255)

                                                                              tctcACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGa                                                                                                                                                                  >  1:517046/1‑251 (MQ=255)

                                                                                 cACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTg                                                                                                                                                                                                      <  1:3979/212‑1 (MQ=255)

                                                                                 cACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGt                                                                                                                                                                            >  1:214998/1‑238 (MQ=255)

                                                                                   ccTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTc                                                                                                                                                                                 >  1:830629/1‑231 (MQ=255)

                                                                                   ccTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAattat                                                                                                                                                             >  1:555191/1‑251 (MQ=255)

                                                                                   ccTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAattat                                                                                                                                                             >  1:676923/1‑251 (MQ=255)

                                                                                     tATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAAttatta                                                                                                                                                           <  1:870749/251‑1 (MQ=255)

                                                                                      atgatgGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTAc                                                                                                                                                          <  1:500962/251‑1 (MQ=255)

                                                                                       tgatgGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGt                                                                                                                                                                            <  1:140934/232‑1 (MQ=255)

                                                                                            gCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATgg                                                                                                                                                                                                                   <  1:308858/188‑1 (MQ=255)

                                                                                                 gagaAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGa                                                                                                                                               >  1:155500/1‑251 (MQ=255)

                                                                                                    aaaTGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAg                                                                                                                                              <  1:687761/249‑1 (MQ=255)

                                                                                                        ggTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTg                                                                                                                                                                                                      <  1:819897/189‑1 (MQ=255)

                                                                                                         gTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCACATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCaaa                                                                                                                                       >  1:330690/1‑251 (MQ=255)

                                                                                                            tgttgCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGc                                                                                                                                    <  1:20418/251‑1 (MQ=255)

                                                                                                              ttgCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCt                                                                                                                                                                                                                                      <  1:831602/151‑1 (MQ=255)

                                                                                                              ttgCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTcc                                                                                                                                                                                                                       <  1:281961/166‑1 (MQ=255)

                                                                                                                 ccACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACAt                                                                                                                                <  1:189331/250‑1 (MQ=255)

                                                                                                                  cACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGCCCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTc                                                                                                                              <  1:798325/251‑1 (MQ=255)

                                                                                                                      cTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAAt                                                                                                                                                                                                                                           >  1:633779/1‑138 (MQ=255)

                                                                                                                        gCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGCTTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAg                                                                                                                                              >  1:455415/1‑229 (MQ=255)

                                                                                                                        gCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAAttatta                                                                                                                                                           <  1:547312/216‑1 (MQ=255)

                                                                                                                           ctTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCt                                                                                                                                                                                                                                      >  1:702449/1‑138 (MQ=255)

                                                                                                                           ctTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTc                                                                                                                                                                                 <  1:177990/191‑1 (MQ=255)

                                                                                                                           ctTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCg                                                                                                                     >  1:489367/1‑251 (MQ=255)

                                                                                                                            ttttACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGa                                                                                                                    <  1:357252/251‑1 (MQ=255)

                                                                                                                             tttACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGat                                                                                                                   >  1:407524/1‑251 (MQ=255)

                                                                                                                              ttACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAGATGCACATTCAGTCCGGCGata                                                                                                                  <  1:155391/251‑1 (MQ=255)

                                                                                                                                  gTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTg                                                                                                               >  1:107174/1‑250 (MQ=255)

                                                                                                                                       gctCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTAc                                                                                                                                                          <  1:777146/202‑1 (MQ=255)

                                                                                                                                         tctTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCtat                                                                                                                                                                                      <  1:608928/172‑1 (MQ=255)

                                                                                                                                         tctTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTAc                                                                                                                                                          <  1:408469/200‑1 (MQ=255)

                                                                                                                                               cccTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATg                                                                                                          >  1:631217/1‑242 (MQ=255)

                                                                                                                                                  tttCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGt                                                                                                                                                                            <  1:172824/173‑1 (MQ=255)

                                                                                                                                                  tttCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATg                                                                                              <  1:305457/251‑1 (MQ=255)

                                                                                                                                                       aTTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGcc                                                                                                             >  1:784775/1‑231 (MQ=255)

                                                                                                                                                       aTTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGtttt                                                                                          >  1:611796/1‑250 (MQ=255)

                                                                                                                                                       aTTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGtttt                                                                                          >  1:359115/1‑250 (MQ=255)

                                                                                                                                                         tCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTccc                                                                                       <  1:672683/251‑1 (MQ=255)

                                                                                                                                                          cTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGc                                                                                                                                                                                         <  1:207829/152‑1 (MQ=255)

                                                                                                                                                          cTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTcc                                                                                        >  1:124695/1‑249 (MQ=255)

                                                                                                                                                            gTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAatta                                                                                                                                                              >  1:31298/1‑177 (MQ=255)

                                                                                                                                                                                     tttGATAACCCGAGCCGCATTCTCTTTTTTCTGGCCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAg                                                                                                                                              <  1:350112/168‑1 (MQ=255)

                                                                                                                                                                                                          ctctTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACAt                                                                                                                                                        >  1:813655/1‑137 (MQ=255)

                                                                                                                                                                                                                 ttCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTatat                               <  1:11374/251‑1 (MQ=255)

                                                                                                                                                                                                                 ttCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTatat                               <  1:198586/251‑1 (MQ=255)

                                                                                                                                                                                                                      aCCTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTg                           <  1:733073/250‑1 (MQ=255)

                                                                                                                                                                                                                       ccTTGTTGCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAg                                                                                                                                              >  1:215124/1‑134 (MQ=255)

                                                                                                                                                                                                                          tgttgCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTc                      <  1:8293/251‑1 (MQ=255)

                                                                                                                                                                                                                          tgttgCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTc                      <  1:531859/251‑1 (MQ=255)

                                                                                                                                                                                                                             tgCCGTTACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTCAGt                   <  1:315066/251‑1 (MQ=255)

                                                                                                                                                                                                                                   tACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTCAGTCTGATg             <  1:226526/251‑1 (MQ=255)

                                                                                                                                                                                                                                   tACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTCAGTCTGATg             <  1:1870/251‑1 (MQ=255)

                                                                                                                                                                                                                                   tACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTCAGTCTGATg             <  1:506065/251‑1 (MQ=255)

                                                                                                                                                                                                                                   tACTGGCGCATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATATTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTCAGTCTGATg             >  1:443872/1‑251 (MQ=255)

                                                                                                                                                                                                                                         cgcATTATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTCAGTCTGATGGGCgtg       >  1:630997/1‑251 (MQ=255)

                                                                                                                                                                                                                                             ttATCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCt                                                                                                                                                                     >  1:127535/1‑89 (MQ=255)

                                                                                                                                                                                                                                               aTCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGt                                                                                                                                                                            >  1:415574/1‑80 (MQ=255)

                                                                                                                                                                                                                                               aTCCGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTc                                           <  1:74235/209‑1 (MQ=255)

                                                                                                                                                                                                                                                 ccGATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTc                                                                                                        <  1:230741/146‑1 (MQ=255)

                                                                                                                                                                                                                                                   gATACGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGc                                                                            >  1:309613/1‑172 (MQ=255)

TAACTATACCCCGGCAATCTTGTCGCTGATGGGCGTAATTTTTTTCATTTACCGCCAATTATGCAAACCGAAGGGCAATCTCACCTATGATGGCAATGAGAAATGGTTTGTTGCCACCCTGCTCTTTTACGTGCTGCTCTTTGCCCTTTCGATTCTGTACCATCAGGGCAAAATTCGGGAATTTGATAACCCGAGCCGCATTCTCTTTTTTCTGACCTTGTTGCCGTTACTGGCGCATTATCCGATATGTTTTAATTGGCTTTTACACGCCATTCCATGGGGCAGCCTGATTGCGGGCATCACCGCTATTATTCATCGTTTTATCTTGAATTATTACATGGCATTTGAGTATCAAATGCACATTCAGTCCGGCGATATTGCCATGTCGTTGGGCATGTTTTCCCTGGCGATTAGCTTTTACTTTTTCATCAAAAGGCAATATCACTTCGGTGCCTTATATTTTGTATTCAGTCTGATGGGCGTGTTCGC  >  1/531943‑532431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`