Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	524,947	T→C	intergenic (+102/‑9)	unknown → / → unknown	hypothetical protein/Mobile element protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	524,947	0	T	C	100.0%	11.4 / NA	6	intergenic (+102/‑9)	unknown/unknown	hypothetical protein/Mobile element protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/2);  total (4/2)

GATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAATTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACG  >  1/524710‑525026                                                                                                                                                                                                                                              |                                                                                gATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATggg                                                                    >  1:331571/1‑251 (MQ=11)                ttatcAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCa                                                     <  1:670571/247‑1 (MQ=255)                                         aaaCAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAAt                            >  1:182686/1‑251 (MQ=255)                                              ggTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATGCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGtt                       >  1:246851/1‑251 (MQ=255)                                                           gATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTattcattc           <  1:470977/250‑1 (MQ=255)                                                                    aTCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAACTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACg  >  1:797321/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                GATTATGTCTTACTCTTACCAATTTCGTTTAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGAAGCCAAAGATGAAAAAGAAAGCGATTGAAATCCCGGAACAAATTACGTCAAATGGGTTTCCATTTGAATCATAAAACGGTGTTAAAACTGATGAATGCGTTAGGTATTCATTCTATTTTACG  >  1/524710‑525026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑